Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pTK5 (#RDB01027)

HSV I thymidine kinase

Alternative name HSV I thymidine kinase
Clone info. Herpes simplex virus type I thymidine kinase
Comment At least covering 46589..47442 and 47550..47719 of GU734771.1. Reference for insert sequence, Wigler, M., Cell, 14 (3): 725-731 (1978).
Vector backbone pBR322 (Plasmid)
Size of vector backbone 4,361 bp
Selectable markers Amp^r
Gene/insert name HSV thymidine Kinase Genomic DNA
Depositor|Developer Soeda, Eiichi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01027 pTK5 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pTK5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01027).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12799_A4F2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_F
Sequence file: RDB12799_A4F2a.seq check
>01027_12799_A4F2_pBR322_BamHI_F_B10_04.ab1
    1 GAAGGTTCTG TGGGATCAAT GAGTCTTCGG ACCTCGCGGG GGCCGCTTAA GCGGTGGTTA
   61 GGGTTTGTCT GACGCGGGGG GAGGGGGAAG GAACGAAACA CTCTCATTCG GAGGCGGCTC
  121 GGGGTTTGGT CTTGGTGGCC ACGGGCACGC AGAAGAGCGC CGCGATCCTC TTAAGCACCC
  181 CCCCGCCCTC CGTGGAGGCG GGGGTTTGGT CGGCGGGTGG TAACTGGCGG GCCGCTGACT
  241 CGGGCGGGTC GCGCGCCCCA GAGTGTGACC TTTTCGGTCT GCTCGCAGAC CCCCGGGCGG
  301 CGCCGCCGCG GCGGCGACGG GCTCGCTGGG TCCTAGGCTC CATGGGGACC GTATACGTGG
  361 ACAGGCTCTG GAGCATCCGC ACGACTGCGG TGATATTACC GGAGACCTTC TGCGGGACGA
  421 GCCGGGTCAC GCGGCTGACG CGGAGCGTCC GTTGGGCGAC AAACACCAGG ACGGGGCACA
  481 GGTACACTAT CTTGTCACCC GGAGGCGCGA GGGACTGCAG GAGCTTCAGG GAGTGGCGCA
  541 GCTGCTTCAT CCCCGTGGCC CGTTGCTCGC GTTTGCTGGC GGTGTCCCCG GAAGAAATAT
  601 ATTTGCATGT CTTTAGTTCT ATGATGACAC AAACCCCGCC CAGCGTCTTG TCATTGGCGA
  661 ATTCGAACAC GCAGATGCAG TCGGGGCGGC GCGGTCCCAG GTCCACTTCG CATATTAAGG
  721 TGACGCGTGT GGCCTCGAAC ACCGAGCGAC CCTGCAGCGA CCCGCTTAAC AGCGTCAACA
  781 GCGTGCCGCA GATCTTGGTG GCGTGAAACT CCCGCACCTC TTCGGCAAGC GCCTTGTAGA
  841 AGCGCGTATG GCTTCGTACC CCTGCCATCA ACACGCGTCT GCGTTCGACC AGGCTGCGCG
  901 TTCTCGCGGC CATAGCAACC GACGTACGGC GTTGCGCCCT CGCCGGCAGC AAGAAGCCAC
  961 GGAAGTCCGC CTGGAGCAGA AAATGCCCAC GCTACTGCGG GTTTATATAG ACGGTCCCTC
 1021 ACGGGATGGG GGAAAACCCA CCCACCACGC AACTGCTGGG TGGGCCCTGG GGCTCGCGCG
 1081 ACGATATCGT CTACGTTACC CGAGCCGAAT GACTTACTGC CAAGTGCCTG GGGGCATCTC
1141 GGACATCGCG ACATTCTTAT ACAATCCTC
//
Primer: pBR322_BamHI_R
Sequence file: RDB12799_A4F2b.seq check
>01027_12799_A4F2_pBR322_BamHI_R_C10_07.ab1
    1 GCGTTGAGAA ACACAGAATC CGCGGCACAG CCCGCGGCCC CGCGGGCGGC CAACCCGGCA
   61 AGCGCGCGCG AGTGGGCCAA AAAGCCTAGC AGGTCGGAGA GGCAGACCGC GCCGTTTGCG
  121 TGGGCGGCGT TCACGAAAGC AAAACCCGAC GTCGCGAGCA GCCCCGTTAG GCGCCAGAAG
  181 AGAGGGGGGC GCGGGCCCTG CTCGGCGCCC GCGTCCCCCG AGAAAAACTC CGCGTATGCC
  241 CGCGACAGGA ACTGGGCGTA GTTCGTGCCC TCCTCCGGGT AGCCGCCCAC GCGGCGGAGG
  301 GCGTCCAGCG CGGAGCCGTT GTCGGCCCGC GTCAGGGACC CTAGGACAAA GACCCGATAC
  361 CGGGGGCCGC CCGGGGGCCC GGGAAGAGCC CCCGGGGGGT TTTCGTCCGC GGGGTCCCCG
  421 ACCCGATCTA GCGTCTGGCC CGCGGGGACC ACCATCACTT CCACCGGAGG GCTGTCGTGC
  481 ATGGATATCA CGAGCCCCAT GAATTCCCGC CCGTAGCGCG CGCGCACCAG CGCGGCATCG
  541 CACCCGAGCA CCAGCTCCCC CGTCGTCCAG ATGCCCACGG GCCACGTCGA GGCCGACGGG
  601 GAGAAATACA CGTACCTACC TGGGGATCTC AACAGGCCCC GGGTGGCCAA CCAGGTCGTG
  661 GACGCGTTGT GCAGGTGCGT GATGTCCAGC TCCGTCGTCG GGTGCCGCCG GGCCCCAACC
  721 GGCGGTCGGG GGGGCGGTGT ATCACGCGGC CCCGCTTGGG TGGCTCGCCC GTCGCCACGT
  781 TTGTCTCCCC GCGGGGAACG TCAGGGCCCT CGGGTTCAGG GACGGCCGAA AACGTTACCC
  841 AGGCCCGGGA ACGGCAACTA CACGGAAGCG ACTGGATTTG TACAATAGAC CCCTTAAGGG
  901 GGCCGACCGA AGGGGGGGAG GGCTGGGCCG GTTCGGCTCG ACCGTGGTCG GGGGCGGGCA
  961 GGCTCGCCGT TTCCGGGGGC CCGGGCTGAA GCAGCGTAAG GTCCTTCGGG CACTGTAAAA
1021 GGAA
//
Primer: HSV_TKpA_R
Sequence file: RDB12799_A4F2c.seq check
>01027_12799_A4F2_HSV_TKpA_R_C10_07.ab1
    1 ACGGGGTTCT TCCGGGTATT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCATCTCCCG
   61 GGCAAACGTG CGCGTCTCAG GTCGCAGATC GTCGGTATGG AGCCGGGGGT GGTGACGTGG
  121 GTCTGGACCA TCCCGGAGGT AAGTTGCAGC AGGGCGTCCC GGCAGCCGGC GGGCGATTGG
  181 TCGTAATCCA GGATAAAGAC GTGCATGGGA CGGAGGCGTT TGGCCAAGAC GTCCAAGGCC
  241 CAGGCAAACA CGTTGTACAG GTCGCCGTTG GGGGCCAGCA ACTCGGGGGC CCGAAACAGG
  301 GTAAATAACG TGTCCCCGAT ATGGGGTCGT GGGCCCGCGT TGCTCTGGGG CTCGGCACCC
  361 TGGGGCGGCA CGGCCGTCCC CGAAAGCTGT CCCCAATCCT CCCGCCACGA CCCGCCGCCC
  421 TGCAGATACC GCACCGTATT GGCAAGCAGC CCGTAAACGC GGCGAATCGC GGCCAGCATA
  481 GCCAGGTCAA GCCGCTCGCC GGGGCGCTGG CGTTTGGCCA GGCGGTCGAT GTGTCTGTCC
  541 TCCGGAAGGG CCCCCAACAC GATGTTTGTG CCGGGCAAGG TCGGCGGGAT GAGGGCCACG
  601 AACGCCAGCA CGGCCTGGGG GGTCATGCTG CCCATAAGGT ATCGCGCGGC CGGGTAGCAC
  661 AGGAGGGCGG CGATGGGATG GCGGTCGAAG ATGAGGGTGA GGGCCGGGGG CGGGGCATGT
  721 GAGCTCCCAG CCTCCCCCCC GATATGAGGA GCCAGAACGG CGTCGGTCAC GGCATAAGGC
  781 ATGCCCATTG TTATCTGGGC GCTTGTCATT ACCACCGCCG CGTCCCCGGC CGATATCTCA
  841 CCCTGGTCGA GGCGGTGTTG TGTGGTGTAG ATGTTCGCGA TTGTCTCGGA AGCCCCCCAG
  901 CACCTGCCAG TAAGTCATCG GCTCGGGGTA CGTAGACGAT ATCGTCGCGC GAACCCAGGG
  961 CCACCAGCAG TTGCGTGGTG GTGGTTTTCC CCATCCCGTG AGGACCGTTC TATATAAACC
 1021 CGCAGTTAGC GTGGGCAATT TTTCTGCTTC CAGGCGGACT TTCCCGTGGC CTTCCTTGCT
 1081 TGCCCGGGCC GAGGGGGCGC CAAACGGTCG TACGATCCGA TTGCCTTATG GTCGGCCGAG
1141 GAAACCGCCT GGCCAGAGCC CCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Wigler, M., Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor. Cell, 14 (3): 725-731 (1978). PMID 210957.
user_report Wada, K.I., Generation of transmitochondrial cybrids using a microfluidic device. Exp. Cell Res. 418 (1): 113233 (2022). PMID 35659971. [link to RRC of NBRP]
user_report Kurayoshi, K., Cancer cell specific cytotoxic gene expression mediated by ARF tumor suppressor promoter constructs. Biochem. Biophys. Res. Commun., 450 (1): 240-246 (2014). PMID 24893334. [link to RRC of NBRP]