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pco12 EGFR (#RDB01276)

Human EGF receptor (EGFR) cDNA

Alternative name Human EGF receptor cDNA
Clone info. Human EGF receptor (EGFR) cDNA
Vector backbone pCO12 (Plasmid)
Size of vector backbone 11.2 kb
Selectable markers Amp^r
Gene/insert name Human EGFR cDNA
Depositor|Developer Yamamoto, Tadashi |
 
Additional map
Remarks, protocol and/or map (pdf) RDB01276.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01276 pco12 EGFR DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pco12 EGFR was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01276).

Reference section:

Velu, T.J., Beguinot, L., Vass, W.C., Zhang, K., Pastan, I., Lowy, D.R. , Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908. [link to RRC of NBRP]
Velu, T.J., Beguinot, L., Vass, W.C., Willingham, M.C., Merlino, G.T., Pastan, I., Lowy, D.R., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18645_B0Lfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro_R1 (Pr0762)
Region: pBR322-BamHI-Ha MuSV (LTR)
Sequence file: RDB18645_B0Lfa.seq check
>01276_18645_B0Lf_1_lacIpro_R1_F02_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGCTCNTGAG CCCGAAGTGG CGAGCCCGAT
   61 CTTCCCCATC GGTGATGTCN GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA
  121 TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCCGGATCTC CCTCTCCTCC AACCACCCTT
  181 GGAACGTCTT CCAGGGCTGC GGGGGAGAAG TCCGAGCTCG TCAGCTCCTT CTCTGGCCCG
  241 CCCAGATAGT CCCCAGATCT GAGCCTATTG ATCGATCGTT CACAGGACAA GACACAACAG
  301 ACAAGACAAA CGCCGGCCGG CTTGCCTCCC GGTGGTGGGT CGGTGGTCCC TGGGCAGGGG
  361 TCTCCCGATC CCGGACGAGC CCCCAAATGA AAGACCCCCA CTAACGGGTA GTCAATCACT
  421 TAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  481 TATTGGATAT ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG
  541 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
  601 GAACTGATTG GTTAGTTTNA ATNANGNACA GGGTCATTTC AGGTCCNTGG GGNACCCTNN
  661 AAACNTCNGA NGNNTCTCTN NNNNTGCTGN NGNNTNNNNN GCNNCTGGGG NNCATCTNNN
721 CNNGNNCNNN NNNNNGGNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: pBR322 (AmpR pro)-HindIII-Ha MuSV
Sequence file: RDB18645_B0Lfb.seq check
>01276_18645_B0Lf_1_Amp_R_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNANNNGNN NCATTTATNA GGGTTATTGT CTCATGNAGC GGANACATAT
   61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
  121 CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA
  181 CGAGGCCCTT TCGTCTTCAA GAATTAATTC TCATGTTTGA CAGCTTATCA TCGATAAGCT
  241 TTCCTCACCA AACGAGAGAC GGCCTCTACA ATCATCAAGA AGATACTGGA AGAAATCTTC
  301 CTCTTGGAAT GCCCAAGGTA ATCTGGTCAG ACAACGGCCC TACTTTCGTT GCCAAGGTAA
  361 GCCAGGGTGT GGCCAAGTAT TTAGAGGTCG ATTGAAAATT ACATTGTATT TACAGACCTC
  421 AAAGTTCAGG ACAGGTAGGG TAAATAAATA AAACTCTAAA AGAGACCCTG ACCAAATTAA
  481 CCATGGAGAC TGGCACAGAC TGGGTGACAC TCCTTCCCTC TTGCTCTCTT CAGAGCAAGA
  541 AATACCCCTT CCAGATTCNG CCTTACCCCC TTTGAGATCT TATATGGGGC CTCAGCCCCA
  601 CTGACTGTAT TANATGANGT TACTGAACCN ACATGTCATA GTGNCATAGT AATAATGANT
  661 TGTGTGNNNN GNNNAAANAN CTACANGNGN NACNGAAAGA AATCTGNTNN NNNNNNGNAN
721 NNNNNNANNN CNNNGGNNNC CNNNNNNNNN N
//
Primer: ESFR-N_rev1 (Pr0576)
Region: insert 5'(hEGFR 5')-SacII-Ha MuSV
Sequence file: RDB18645_B0Lfc.seq check
>01276_18645_B0Lf_1_ESFR-N_rev1_C01_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNC CTCCNNGNNG GTCTTTAAGA AGGAAAGANC ATAATTCCTC
   61 TGCACATAGG TAATTTCCAA ATTCCCAAGG ACCACCTCAC AGTTATTGAA CATCCTCTGG
  121 AGGCTGAGAA AATGATCTTC AAAAGTGCCC AACTGCGTGA GCTTGTTACT CGTGCCTTGG
  181 CAAACTTTCT TTTCCTCCAG AGCCCGACTC GCCGGGCAGA GCGCAGCCAG CAGCGCCAGG
  241 AGCGCTGCCC CGGCCGTCCC GGAGGGTCGC ATCGCTGCTC CCCGAAGAGC TCGCTCCGGC
  301 TCTCCCGATC AATACTGGAC GGAGTCAGGG GGCCGTGCGC GGTGGTTGTG GCGTTGGCGG
  361 CGAGGCGGGG ACTCGGGCGG ACGCCGACGA GGTGGCCTGT CGTCCGGTCT GGGCGGCGGC
  421 GGCCGCCGGG GCCATCGACT CTAGCGGATC CGCGGCGGGT GCGGCTCGGG CTGCGGGCAT
  481 AAGCACAGAT AAAACACTAT ATGTTTTTGT TTTGTTTCTC TTTCCTTTTT TTCAGAGCTG
  541 GGGACCGAAC CCAGGGCCTT GCGCTTGCTA GGCAAGAGCT CTATCACTGA NCTAAATCCC
  601 CAACCCCCAA AACACTATGT TTTAAAAATT AACTTTGGCT ATCNACNNAC ACACTGCCNC
  661 TAGAGCGGGG NCTACAAAAN TNNANTTTCN TANTCNCTAA NCNNNNANNN NNNNNANTAA
721 ANCTNNTNNN NNNANNNAAN NNNAANNNNN TTNNNNNNNN NN
//
Primer: pCO12_R (Pr0240)
Region: Ha MuSV-XhoI-insertt 3' (hEGFR 3')
Sequence file: RDB18645_B0Lfd.seq check
>01276_18645_B0Lf_1_pCO12_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNGCCGGGNN GGGCACAAAG GNCATGACTG ACCCTCGAGA
   61 CATGGGCCTG CAGGTCGATG GAAAGATAAA TTTCTGCTCA AAGGGACAAT ATTCTTGCTG
  121 GATGCGTTTC TGTAAATGCT TCACAGTTTG AAGACAAAGG AATGCAACTT CCCAAAATGT
  181 GCCCGAGGTG GAAGTACTTC CTGGCTAGTC GGTGTAAACG TTGCAAAACC AGTCTGTGGG
  241 TCTAAGAGCT AATGCGGGCA TGGCTGTTGG GATGGAGGAC CTGCTGTGGC TTGGTCCTGG
  301 GTATCGAAAG AGTCTGGATT TTTAGGGCTC ATACTATCCT CCGTGGTCAT GCTCCAATAA
  361 ATTCACTGCT TTGTGGCGCG ACCCTTAGGT ATTCTGCATT TTCAGCTGTG GAGCCCTTAA
  421 AGATGCCATT TGGCTTGGCT TCCTTGGGAA AGAAGTCCTG CTGGTAGTCA GGGTTGTCCA
  481 GGCTAATTTG GTGGCTGCCT TTCTGGGCCC AGTGGGCAGG GCTGTCGAAT GTGCTGTTGA
  541 CACAGGTGGG CTGGACAGTG TTGAGATACT CGGGGTTGCC CACTGCAGTG CTGTGGGGGT
  601 CCTGGTAGTG TGGGTCTCTG CTGGGCGCGG GGTTCANANG CTGANTGTGA TANACAGGAN
  661 TCTGCNCAGA NCCNGCGGGN CNTTTGGNAA CGGACTNNTT TANGNNTTNN NNNNCTGGGN
721 NNNNANGNNG TCNNNNN
//
Primer: ESFR-C_F1 (Pr0938)
Region: insert 3'-XhoI-Ha MuSV
Sequence file: RDB18645_B0Lfe.seq check
>01276_18645_B0Lf_1_ESFR-C_F1_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TCCNGNNNNN NNATTGTCCC TTTGANCAGA AATTTATCTT
   61 TCCNTCGACC TGCAGGCCCA TGTCTCGAGG GTCAGTCATG TCCTTTGTGC CCGTCCCGGC
  121 ACAGGCTCAG GACGAGGAGG TGCCGGATGC AGGGAGGTAG GTACTCCTTA AGGGAAAATC
  181 TAAGCCCAGA GAGACAGCCG GTAACAGCTA GGCTGCCTCT CTGCTCGCCT TTCTGTTTCA
  241 TAGACAAAGC TTGTGCTTAT ATTTACAGTG GCCTTTTTGT CCAAAAAAAG GCCTCCTGGT
  301 TTAGCTGTGT GACTAAATGC TATTTGTCCT CTTCAGTGGA CCTCTCTCCT CTAGATTCCC
  361 TTCTTGTTTT CTCACCATCC CACGTGAGAT GCTTGTTAGT AACTGTTACA GATCTTCTTT
  421 ACCACTGAGG AAAGACAGAA TCCTGCTAAA GGCCAGAAAG AATGTTCCAG ACATGGATGG
  481 AAGGCTCCTG ACTGTTGACT TCAATGCCCC TGAAGGTAGG GAGTGCTCCA GGTCTGCCCC
  541 CAGGCTCCGA GGGTGGGTCT CCTAGGGGCT AGAAAATGCC CCACNNATCT GGCTAAGATA
  601 AGAAAAAGAT ATAAAGAGAA AGTTACNGAA ACTTGAAGGG TAAAGCTAAG TCACTGAAAG
  661 AGNTATTATA AGTTACAGAN AAAATAANTT NANGCNNNNN NNCNNNNNNG NGNNNNAANN
721 NNNNNNNN
//
Primer: HaMuSV-3pri_R2 (Pr 0946)
Region: Ha MuSV (LTR)
Sequence file: RDB18645_B0Lff.seq check
>01276_18645_B0Lf_1_HaMuSV-3pri_R2_E02_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNAGTAAAAC TCTTCGACGA ACAAAGCAAA CAGTTTCATG
   61 ATTTCAAACA CAGTCGTCGG TCTAAGATTT TAAACACAGT CGTCAGTCCA AATTCAAACA
  121 CGAAACAAAA GTCAAAAAGA CACTAACAGA CACAACACGT CCAGAAAACC ACAGTCAGGT
  181 CACAAAGAAG ACAAACAATT CCAACAGTCA AACAAGTAAC AAGCAGACGC GCCGCGCAGC
  241 TTCGGTACCA AACTGAAACC AAAAATTCAG ACGGAGTCAT CAAGGGTGCG GATCCCTCCG
  301 AAAACGGACG GAGGTGCCAC GGATCCGGAT CTCCCTCTCC TCCAACCACC CTTGGAACGT
  361 CTTCCAGGGC TGCGGGGGAG AAGTCCGAGC TCGTCAGCTC CTTCTCTGGC CCGCCCAGAT
  421 AGTCCCCAGA TCTGAGCCTA TTGATCGATC GTTCACAGGA CAAGACACAA CAGACAAGAC
  481 AAACGCCGGC CGGCTTGCCT CCCGGTGGTG GGTCGGTGGT CCCTGGGCAG GGGTCTCCCG
  541 ATCCCGGACG AGCCCCCAAA TGAAAGACCC CCACTAACGG GTAGTCAATC ACTTAGAGGA
  601 GACCCTCCCN AGGAACAGCG AGACCACAAG TCGGANGNNA CTGCNAGANG GTTTATTGNA
  661 NATACGGGTA CCCGGGCGAN TCNGTCNATC NNNNNANTGG NNNCCCNNNN GNGGGGNNGN
721 GGNNNNNTTN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Velu, T.J., Retroviruses expressing different levels of the normal epidermal growth factor receptor: biological properties and new bioassay. J. Cell Biochem., 39, 153-166 (1989). PMID 2565908. [link to RRC of NBRP]
original Velu, T.J., Epidermal-growth-factor-dependent transformation by a human EGF receptor proto-oncogene. Science, 238, 1408-1410 (1987). PMID 3500513. [link to RRC of NBRP]
reference Clark, A.J., Synthesis of epidermal growth factor (EGF) receptor in vitro using SP6 RNA polymerase-transcribed template mRNA. Biochim. Biophys. Acta., 867, 244-251 (1986). PMID 3017432.
user_report Su, J., Transmembrane signaling on a protocell: Creation of receptor-enzyme chimeras for immunodetection of specific antibodies and antigens. Sci. Rep. 9 (1): 18189 (2019). PMID 31796769. [link to RRC of NBRP]
user_report Fukuda, N., Rapid Evaluation of Tyrosine Kinase Activity of Membrane-Integrated Human Epidermal Growth Factor Receptor Using the Yeast G gamma Recruitment System. ACS Synth. Biol. 4 (4): 421-429 (2015). PMID 25006755. [link to RRC of NBRP]
user_report Kojima, K., ESCRT-0 Protein Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate (Hrs) Is Targeted to Endosomes Independently of Signal-transducing Adaptor Molecule (STAM) and the Complex Formation with STAM Promotes Its Endosomal Dissociation. J. Biol. Chem. 289 (48): 33296-33310 (2014). PMID 25296754. [link to RRC of NBRP]
user_report Hiragun, T., Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines. Biosens. Bioelectron., 32 (1): 202-207 (2012). PMID 22204782. [link to RRC of NBRP]
user_report Tanaka, K., Selection and growth regulation of genetically modified cells with hapten-specific antibody/receptor tyrosine kinase chimera. Biotechnol. Progr., 25, 1138-1145 (2009). PMID 19569211. [link to RRC of NBRP]
user_report Dyson M.R., Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression BMC Biotechnol., 4, 1-18 (2004). PMID 15598350. [link to RRC of NBRP]
user_report Niikura T., Insulin-like growth factor I (IGF-I) protects cells from apoptosis by Alzheimer's V642I mutant amyloid precursor protein through IGF-I receptor in an IGF-binding protein-sensitive manner. J. Neurosci., 21, 1902-1910 (2001). PMID 11245675. [link to RRC of NBRP]
user_report Fujino T., Enhanced expression of heparin-binding EGF-like growth factor and its receptor in hypertrophied left ventricle of spontaneously hypertensive rats. Cardiovasc. Res., 38, 365-374 (1998). PMID 9709397. [link to RRC of NBRP]
user_report Nakamura N., An epidermal growth factor receptor/Jak2 tyrosine kinase domain chimera induces tyrosine phosphorylation of Stat5 and transduces a growth signal in hematopoietic cells. J. Biol. Chem., 271, 19483-19488 (1996). PMID 8702638. [link to RRC of NBRP]