Please review the QC test results indicated by icon below as well as clone information before placing your order.

AxCANCre (#RDB01748)

Recombinant adenovirus expressing recombinase Cre with nuclear localization signal

Alternative name
Clone info.	Recombinant adenovirus expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter.
Vector backbone	pAxcw (RDB00917) (Adenovirus)
Size of vector backbone	-
Growth conditions	37 C, DMEM/10 percent FCS, 5 percent CO2
Growth remarks	5 percent FCS is fine.
Gene/insert name	P1 phage Cre recombinase cDNA
Depositor|Developer	Saito, Izumu |

 

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Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01748	AxCANCre	Virus, lysate of infected cell culture	JPY 32,340 plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The AxCANCre was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01748).

Reference section:

Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]

Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB01748_02I4p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328) Region: CAG pro,SV40 NLS,Cre 5' Sequence file: RDB01748_02I4a.seq
>01748_01748_02I4_pCAG-F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNGNNN NNGGNANGGC GGGGTTCGGC TTCTGGCGTG    61 TGANCGGCGG CTCTAGAGCC TCTGCTAACC ATGTTCATGC CTTCTTCTTT TTCCTACAGC   121 TCCTGGGCAA CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTGATTTATC   181 GATTTGCAGA CCGTGCATCA TGAGCGGCCC TCCAAAAAAG AAGAGAAAGG TAGAAGACCC   241 GGGCGGCCGC ATGTCCAATT TACTGACCGT ACACCAAAAT TTGCCTGCAT TACCGGTCGA   301 TGCAACGAGT GATGAGGTTC GCAAGAACCT GATGGACATG TTCAGGGATC GCCAGGCGTT   361 TTCTGAGCAT ACCTGGAAAA TGCTTCTGTC CGTTTGCCGG TCGTGGGCGG CATGGTGCAA   421 GTTGAATAAC CGGAAATGGT TTCCCGCAGA ACCTGAAGAT GTTCGCGATT ATCTTCTATA   481 TCTTCAGGCG CGCGGTCTGG CAGTAAAAAC TATCCAGCAA CATTTGGGCC AGCTAAACAT   541 GCTTCATCGT CGGTCCGGGC TGCCACGACC AAGTGACAGC AATGCTGTTT CACTGGTTAT   601 GCGGCGGATC CGAAAAGAAA ACGTTGATGC CGGTGAACGT GCAAAACAGG CTCTAGCGTT   661 CGAACGCACT GATTTCGACC AGGTTCGTTC ACTCATGGAA AATAGCGATC GCTGCCANGA   721 TATACGTAAT CTGGCATTTC TGGGGATTGC TTATAACACC CTGTTACGTA TAGCCNAAAT   781 TGCCAGGANC NNGGTTNAAG ATATCTCACG TACTGACNGT GGGAGAANGT TAATCCNTAT   841 NGGCANAACG AAAACGCTGG NNNGCACCGC NNNGTANANA ANGNACTTAN CCTGGGGGGT   901 ANNTNANCNN GNCGAGCNNN NGANTTNCNN NNCTNNNNNN NCTNNNGANN CNNNTAACTA 961 NCNNNNTTNN NNNNNNNN //
Primer: pAxCA_R1 (Pr0165) Region: Cre 3' Sequence file: RDB01748_02I4b.seq
>01748_01748_02I4_pAxCA_R1_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCCNGCNNC CNCCTTCTGA NAGGCAGCCT GCACCTGNNG    61 AGTGAATTGA TTATCGATTC TAGACTAGTT TAATTAATTT CTAGAGCGCT TAATGGCTAA   121 TCGCCATCTT CCAGCAGGCG CACCATTGCC CCTGTTTCAC TATCCAGGTT ACGGATATAG   181 TTCATGACAA TATTTACATT GGTCCAGCCA CCAGCTTGCA TGATCTCCGG TATTGAAACT   241 CCAGCGCGGG CCATATCTCG CGCGGCTCCG ACACGGGCAC TGTGTCCAGA CCAGGCCAGG   301 TATCTCTGAC CAGAGTCATC CTTAGCGCCG TAAATCAATC GATGAGTTGC TTCAAAAATC   361 CCTTCCAGGG CGCGAGTTGA TAGCTGGCTG GTGGCAGATG GCGCGGCAAC ACCATTTTTT   421 CTGACCCGGC AAAACAGGTA GTTATTCGGA TCATCAGCTA CACCAGAGAC GGAAATCCAT   481 CGCTCGACCA GTTTAGTTAC CCCCAGGCTA AGTGCCTTCT CTACACCTGC GGTGCTAACC   541 AGCGTTTTCG TTCTGCCAAT ATGGATTAAC ATTCTCCCAC CGTCAGTACG TGAGATATCT   601 TTAACCCTGA TCCTGGCAAT TTCGGCTATA CGTAACAGGG TGTTATAAGC AATCCCCAGA   661 AATGCCAGAT TACGTATATC CTGGCAGCGA TCGCTATTTT CCATGANTGA ACGAACCTGG   721 TCGAAATCAG TGCGTTCGAA CGCTAGAGCC TGTTTTGCAC GTTCACCNGC ATCNNCGTTT   781 TCTTTTCGGA NCCNNNGCAT AACCAGTGAA ACAGCATTGC TGTCANTTGG TCGTGGCANC   841 NNNGANCGAC NATGAANCAT GNTTAGCTNG NCCNAATGTT GCNNGNNNNN TTTTTTACTG   901 NCANANCNCG CNCCTGNANN NATNNNNNNA NANNCNCNNN NNTCNTNCNN NTTNNNNNGN 961 NNNNNNNTTT NNNNNNNNNN //
Primer: CMV_en_R (Pr0173) Region: CAG promoter 5',the adenovirus genome Sequence file: RDB01748_02I4c.seq
>01748_01748_02I4_CMV_en_R_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNTN NNNNACTAGT CAATAATCAA TGTCGACATC GGTTTAAACG    61 ATTGTCGACT CGCGACGATG GATCTGGAAG GTGCTGAGGT ACGATGAGAC CCGCACCAGG   121 TGCAGACCCT GCGAGTGTGG CGGTAAACAT ATTAGGAACC AGCCTGTGAT GCTGGATGTG   181 ACCGAGGAGC TGAGGCCCGA TCACTTGGTG CTGGCCTGCA CCCGCGCTGA GTTTGGCTCT   241 AGCGATGAAG ATACAGATTG AGGTACTGAA ATGTGTGGGC GTGGCTTAAG GGTGGGAAAG   301 AATATATAAG GTGGGGGTCT TATGTAGTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC   361 ATGAGCACCA ACTCGTTTGA TGGAAGCATT GTGAGCTCAT ATTTGACAAC GCGCATGCCC   421 CCATGGGCCG GGGTGCGTCA GAATGTGATG GGCTCCAGCA TTGATGGTCG CCCCGTCCTG   481 CCCGCAAACT CTACTACCTT GACCTACGAG ACCGTGTCTG GAACGCCGTT GGAGACTGCA   541 GCCTCCGCCG CCGCTTCAGC CGCTGCAGCC ACCGCCCGCG GGATTGTGAC TGACTTTGCT   601 TTCCTGAGCC CGCTTGCAAG CAGTGCAGCT TCCCGTTCAT CCGCCCGCGA TGACAAGTTG   661 ACGGCTCTTT TGGCACAATT GGATTCTTTG ACCCGGGAAC TTAATGTCGT TTCTCAGCAG   721 CTGTTGGATC TGCGCCAGCA NGTTTCTGCC CTGAANGCTT CCTCNNCTCN CAATGCGGTT   781 TAAAACATAA ATAAAAAACC AGACTCTGTT TGGATTTGGN TCAAGCAAGT GTCTNGCTGT   841 CTTTATTTAG GGGTTTNGCG CGCGCNGNAN GNCCNGNNNN AGCNGNCTCN NNCNNNNNNG 901 NNCCNNNNTA TTTTTTNCCN NNACGNNNNN AAGGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]
original	Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference	Hosoya, M., Cochlear Cell Modeling Using Disease-Specific iPSCs Unveils a Degenerative Phenotype and Suggests Treatments for Congenital Progressive Hearing Loss. Cell Rep. 18 (1): 68-81 (2017). PMID 28052261.
reference	Danno, S., PKN2 is essential for mouse embryonic development and proliferation of mouse fibroblasts. Genes Cells 22 (2): 220-236 (2017) PMID 28102564.
reference	Sato, T., JSAP1/JIP3 and JLP regulate kinesin-1-dependent axonal transport to prevent neuronal degeneration. Cell Death Differ. 22 (8): 1260-1274 (2015). PMID 25571974.
reference	Tuvshintugs, B., JSAP1 and JLP are required for ARF6 localization to the midbody in cytokinesis. Genes Cells 19 (9): 692-703 (2014). PMID 25130574.
reference	Fukamachi, K., A novel reporter rat strain that expresses LacZ upon Cre-mediated recombination. Genesis, 51 (4): 268-274 (2013). PMID 23349063. [link to RRC of NBRP]
reference	Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report	Komatsu, K., The G9a histone methyltransferase represses osteoclastogenesis and bone resorption by regulating NFATc1 function. FASEB J. 38 (13): e23779 (2024). PMID 38967255. [link to RRC of NBRP]
user_report	Miyazaki, T., Glutamine protects mouse spermatogonial stem cells against NOX1-derived ROS for sustaining self-renewal division in vitro. Development 150 (20): dev201157 (2023). PMID 36897562. [link to RRC of NBRP]
user_report	Kanatsu-Shinohara, M., Adenovirus-mediated gene delivery restores fertility in congenitally infertile female mice. J. Reprod. Dev. 68(6): 369-376 (2022). PMID 36223953. [link to RRC of NBRP]
user_report	Morimoto, H., ROS amplification drives mouse spermatogonial stem cell self-renewal. Life Sci. Alliance 2 (2). pii: e201900374 (2019). PMID 30940732. [link to RRC of NBRP]
user_report	Morita, M., PKM1 Confers Metabolic Advantages and Promotes Cell-Autonomous Tumor Cell Growth. Cancer Cell 33 (3): 355-367.e7 (2018). PMID 29533781. [link to RRC of NBRP]
user_report	Watanabe, S., In Vivo Genetic Manipulation of Spermatogonial Stem Cells and Their Microenvironment by Adeno-Associated Viruses. Stem Cell Reports 10 (5): 1551-1564 (2018). PMID 29628393. [link to RRC of NBRP]
user_report	Salah, M., An in vitro system to characterize prostate cancer progression identified signaling required for self-renewal. Mol. Carcinog. 55 (12): 1974-1989 (2016). PMID 26621780. [link to RRC of NBRP]
user_report	Chanmee, T., Hyaluronan Production Regulates Metabolic and Cancer Stem-like Properties of Breast Cancer Cells via Hexosamine Biosynthetic Pathway-coupled HIF-1 Signaling. J. Biol. Chem. 291 (46): 24105-24120 (2016). PMID 27758869. [link to RRC of NBRP]
user_report	Kanatsu-Shinohara, M., Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal. Genes Dev. 30 (23): 2637-2648 (2016). PMID 28007786. [link to RRC of NBRP]
user_report	Kurihara, C., An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes. Biotechnol. Rep. (Amst.) 12: 26-32 (2016). PMID 28352551. [link to RRC of NBRP]
user_report	Tanaka, T., The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage. J. Reprod. Dev. 61 (4): 305-316 (2015). PMID 25959802. [link to RRC of NBRP]
user_report	Kanatsu-Shinohara, M., Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal. Proc. Natl. Acad. Sci. U.S.A., 111 (24): 8826-8831 (2014). PMID 24879440. [link to RRC of NBRP]
user_report	Matsushita, Y., Microglia activation precedes the anti-opioid BDNF and NMDA receptor mechanisms underlying morphine analgesic tolerance. Curr. Pharm. Des. 19 (42): 7355-7361 (2013). PMID 23448475. [link to RRC of NBRP]
user_report	Egawa, N., The E1 protein of human papillomavirus type 16 is dispensable for maintenance replication of the viral genome. J. Virol., 86 (6): 3276-3283 (2012). PMID 22238312. [link to RRC of NBRP]
user_report	Kumadaki, S., Inhibition of Ubiquitin ligase F-box and WD repeat domain-containing 7(alpha) (Fbw7(alpha}) causes hepatosteatosis through the Kruppel-like factor 5 (KLF5)/PPAR(gamma)2 pathway, but not SREBP-1c in mice. J. Biol. Chem., 286 (47): 40835-40846 (2011). PMID 21911492. [link to RRC of NBRP]
user_report	Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010). [link to RRC of NBRP]
user_report	Takashima, S., Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects. Biol. Reprod., 81, 155-164 (2009). PMID 19228594. [link to RRC of NBRP]
user_report	Deurholt T., Novel immortalized human fetal liver cell line, cBAL111, has the potential to differentiate into functional hepatocytes. BMC Biotechnol., 9: 89 (2009). PMID 19845959. [link to RRC of NBRP]
user_report	Kanatsu-Shinohara, M., Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin. Cell Stem Cell, 3 (5): 533-542 (2008). PMID 18983968. [link to RRC of NBRP]
user_report	Tomimatsu N., Ku70/80 modulates ATM and ATR signaling pathways in response to DNA double strand breaks. J. Biol. Chem., 282, 10138-10145 (2007). PMID 17272272. [link to RRC of NBRP]
user_report	Takehashi, M., Adenovirus-mediated gene delivery into mouse spermatogonial stem cells. Proc. Natl. Acad. Sci. U. S. A., 104 (8): 2596-2601 (2007). PMID 17299052. [link to RRC of NBRP]
user_report	Dali-Youcef N., Adipose tissue-specific inactivation of the retinoblastoma protein protects against diabesity because of increased energy expenditure. Proc. Natl. Acad. Sci. U S A., 104, 10703-10708 (2007). PMID 17556545. [link to RRC of NBRP]
user_report	Omoteyama K., Activation of connective tissue growth factor gene by the c-Maf and Lc-Maf transcription factors. Biochem. Biophys. Res. Commun., 339, 1089-1097 (2006). PMID 16343439. [link to RRC of NBRP]
user_report	Sakaki-Yumoto M., The murine homolog of SALL4, a causative gene in Okihiro syndrome, is essential for embryonic stem cell proliferation, and cooperates with Sall1 in anorectal, heart, brain and kidney development. Development, 133, 3005-3013 (2006). PMID 16790473. [link to RRC of NBRP]
user_report	Hoekstra R., Assessment of in vitro applicability of reversibly immortalized NKNT-3 cells and clonal derivatives. Cell Transplant., 15, 423-433 (2006). PMID 16970284. [link to RRC of NBRP]
user_report	Gao Z., Delection of the PDGFR-beta Gene Affects Key Fibroblast Functions Important for Wound Healing J. Biol. Chem., 280, 9375-9389 (2005). PMID 15590688. [link to RRC of NBRP]
user_report	Yanagihara M., Ribozymes targeting serine/threonine kinase Akt1 sensitize cells to anticancer drugs. Cancer Sci., 96, 620-6 (2005). PMID 16128748. [link to RRC of NBRP]
user_report	Tanaka S., Mitochondrial impairment induced by poly(ADP-ribose) polymerase-1 activation in cortical neurons after oxygen and glucose deprivation. J. Neurochem., 95, 179-190 (2005). PMID 16181422. [link to RRC of NBRP]
user_report	Nishitai G., Stress induces mitochondria-mediated apoptosis independent of SAPK/JNK activation in embryonic stem cells. J. Biol. Chem., 279, 1621-1626 (2004). PMID 14585831. [link to RRC of NBRP]
user_report	Mizukami H., Separate control of Rep and Cap expression using mutant and wild-type loxP sequences and improved packaging system for adeno-associated virus vector production. Mol. Biotechnol., 27, 7-14 (2004). PMID 15122043. [link to RRC of NBRP]
user_report	Matsuura K., Cardiomyocytes fuse with surrounding noncardiomyocytes and reenter the cell cycle. J. Cell Biol., 167, 351-363 (2004). PMID 15492039. [link to RRC of NBRP]
user_report	Aihara M., Angiogenic endothelium-specific nestin expression is enhanced by the first intron of the nestin gene. Lab. Invest., 84, 1581-1592 (2004). PMID 15502861. [link to RRC of NBRP]
user_report	Suzuki K., Adenovirus-mediated gene transfer of interferon alpha improves dimethylnitrosamine-induced liver cirrhosis in rat model. Gene Ther., 10, 765-773 (2003). PMID 12704415. [link to RRC of NBRP]
user_report	Furuyama T., Forkhead transcription factor FOXO1 (FKHR)-dependent induction of PDK4 gene expression in skeletal muscle during energy deprivation. Biochem. J., 375, 365-371 (2003). PMID 12820900. [link to RRC of NBRP]
user_report	Nishioka K., Immortalization of bone marrow-derived human mesenchymal stem cells by removable simian virus 40T antigen gene: analysis of the ability to support expansion of cord blood hematopoietic progenitor cells. Int. J. Oncol., 23, 925-32 (2003). PMID 12963970. [link to RRC of NBRP]
user_report	Noguchi H., Controlled expansion of human endothelial cell populations by Cre-loxP-based reversible immortalization. Hum. Gene Ther., 13, 321-334 (2002). PMID 11812287. [link to RRC of NBRP]
user_report	Iida A., Cytoplasmic RNA vector derived from nontransmissible Sendai virus. Production and use. Methods Mol. Med., 69, 361-370 (2002). PMID 11987789. [link to RRC of NBRP]
user_report	Kaartinen V., TGFbeta3-induced activation of RhoA/Rho-kinase pathway is necessary but not sufficient for epithelio-mesenchymal transdifferentiation: implications for palatogenesis. Int. J. Mol. Med., 9, 563-70 (2002). PMID 12011971. [link to RRC of NBRP]
user_report	Ohba Y., Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis. EMBO J., 20, 3333-3341 (2001). PMID 11432821. [link to RRC of NBRP]
user_report	Fukazawa T., Accelerated degradation of cellular FLIP protein through the ubiquitin-proteasome pathway in p53-mediated apoptosis of human cancer cells. Oncogene, 20, 5225-5231 (2001). PMID 11526513. [link to RRC of NBRP]
user_report	Kobayashi N., Cre/loxP-based reversible immortalization of human hepatocytes. Cell Transplant., 10, 383-386 (2001). PMID 11549058. [link to RRC of NBRP]
user_report	Yokoo T., Inflamed glomeruli-specific gene activation that uses recombinant adenovirus with the Cre/loxP system. J. Am. Soc. Nephrol., 12, 2330-2337 (2001). PMID 11675409. [link to RRC of NBRP]
user_report	Kaartinen V., Removal of the floxed neo gene from a conditional knockout allele by the adenoviral Cre recombinase in vivo. Genesis., 31, 126-129 (2001). PMID 11747203. [link to RRC of NBRP]
user_report	Araki T., Shp-2 specifically regulates several tyrosine-phosphorylated proteins in brain-derived neurotrophic factor signaling in cultured cerebral cortical neurons. Neurochem., 74, 659-668 (2000). PMID 10646517. [link to RRC of NBRP]
user_report	Kobayashi N., Prevention of acute liver failure in rats with reversibly immortalized human hepatocytes. Science, 287, 1258-1262 (2000). PMID 10678831. [link to RRC of NBRP]

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• P1 phage Cre recombinase and ortholog

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• Establishment of deficient cells using Cre recombinase (English text)
• Establishment of deficient cells using Cre recombinase (Japanese text)
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