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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pTcf7wtluc (#RDB02968)

Reporter plasmid carring TCF-binding consensus sequence.

Clone info. Reporter plasmid carring 7 repeates of the TCF-binding consensus sequence upstream of the IFN-beta basal promoter followed by the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Comment Use pTcf7mtluc (cat.# RDB02969) as a negative control.
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Shimotohno, Kunitada |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02968 pTcf7wtluc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pTcf7wtluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02968).

Reference section:

Ueda, Y., Hijikata, M., Takagi, S., Takada, R., Takada, S., Chiba, T., Shimotohno, K., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17505_A9Ibp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: GL2 (Pr0198)
Region: IFN-b basal pro,Tcf motiff WTx7,SV40p(A),f1 ori
Sequence file: RDB17505_A9Iba.seq check
>02968_17505_A9Ib_GL2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNTT GCGAAGGNTG CAGTTAGAAT GTCCTTTCTC CNTGGGTATG
   61 GCCTATTTAT ATGAGATGGT CCTCTCTCTA TTCAGCGGAT CTCGAGGATC AAAGGCGCGA
  121 TCAAAGGTAG GATCAAAGGG CGGATCAAAG GCGCGATCAA AGGTAGGATC AAAGGGCGGA
  181 TCAAAGGACG CGTAAGAGCT CGGTACCTCC CGGGTTATGT TAGCTCAGTT ACAGTACCAT
  241 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
  301 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
  361 TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT
  421 TTAAAGCAAG TAAAACCTCC ACGTACCTTA ATATTACTTA CTTATCATGG TAGCTTGGGC
  481 TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT
  541 GGCGAATGGC AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
  601 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
  661 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
  721 GTGGACTCNA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
  781 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA NNGCACTAAA TCGGANCCNN
  841 AAAGGGANCC CCCGNNTTAN AGCTTGNCGG GGAAAGCCGG CGAANGTGGC GANAAAGGAA
  901 NGGAANAAAG CNAAAGGANC GGGCGCNANG NCGCTNNNAA GNGTAGCGGN CACNNNGCGC
  961 NNAACCNCCA CACCNNCCGC GNTNNANGNN NCNNNNCAGG NNNNGNNNNN NGNNNNTTTN
 1021 NNGGNNANNG NGNNNNNNNN CCNNNNTNNN TNNNTTTNNN NANNNNNNNN NANNNNNNNN
1081 NN
//
Primer: GV (Pr0748)
Region: Tcf motiff WTx6,IFN-b basal pro,Luc
Sequence file: RDB17505_A9Ibb.seq check
>02968_17505_A9Ib_pGV_Cw_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNCTN ANGCGTCCTT TGNTCCGCCC TTTGATCCTA CCTTTGATCG
   61 CGCCTTTGAT CCGCCCTTTG ATCCTACCTT TGATCGCGCC TTTGATCCTC GAGATCCGCT
  121 GAATAGAGAG AGGACCATCT CATATAAATA GGCCATACCC ATGGAGAAAG GACATTCTAA
  181 CTGCAACCTT TCGCAAGCTT GGCATTCCGG TACTGTTGGT AAAATGGAAG ACGCCAAAAA
  241 CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT
  301 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA
  361 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC
  421 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT
  481 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA
  541 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT
  601 GTTTGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACNAATAAT
  661 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC
  721 GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTAC CAGAGTCCTT
  781 TGATCGTGAC AAAACAATTG CACTGATAAT GAATTCCTCT GGATCTACTG GGTTACNTAA
  841 NGGTGTGGCC NTTCCGCATA GAACTGCCTG CGTCAGANTC TCGCATGCCN GANATCCNAT
  901 TTTTGGNAAT CAAATCNTTN CGGANACTGC GATTTTAAGT GNNGNNCCNT TCCATCNCGG
  961 NTTNNGAATG NTNACNACNC NNNGANANTN GANNTGNNNN NTNNGNNNNN NNNTNNNNGN
1021 ATANNNTNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [link to RRC of NBRP]
user_report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481. [link to RRC of NBRP]