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pTcf7mtluc (#RDB02969)

Reporter plasmid carring TCF-binding consensus sequence

Clone info. Reporter plasmid carring 7 repeats the TCF-binding mutant sequence upsteam of the IFN-beta basal promoter followed the luciferase geme. p55Bluc was made by inserting the minimam promoter sequences of human IFNbeta gene into the MCS of PGV-B (TOYO B-Net CO., LTD). Expression was confirmed by depositor as follows: reporter assay.
Comment Use as a negative control of pTcf7wtluc (cat.# RDB02968).
Vector backbone p55Bluc (Plasmid)
Size of vector backbone 5,700 bp
Selectable markers Amp^r
Gene/insert name firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Shimotohno, Kunitada |
 
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Terms and conditions for distribution This material is provided with written consent of Depositor. Acknowledgement to Depositor in publication.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02969 pTcf7mtluc DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pTcf7mtluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02969).

Reference section:

Ueda, Y., Hijikata, M., Takagi, S., Takada, R., Takada, S., Chiba, T., Shimotohno, K., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17506_A9Ibp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: GL2 (Pr0198)
Region: IFN-b basal pro,Tcf motiff mtx7,SV40p(A),f1 ori
Sequence file: RDB17506_A9Iba.seq check
>02969_17506_A9Ib_GL2_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNAGCTTGN NNNNGNTGCA GTTAGAATGT CCTTTCTCCN TGGGTATGGC
   61 CTATTTATAT GAGATGGTCC TCTCTCTATT CAGCGGATCT CGAGGGCCAA AGGCGGGGCC
  121 AAAGGTAGGG CCAAAGGGCG GGCCAAAGGC GCGGCCAAAG GTAGGGCCAA AGGGCGGGCC
  181 AAAGGACGCG TAAGAGCTCG GTACCTCCCG GGTTATGTTA GCTCAGTTAC AGTACCATAA
  241 GATACATTGA TGAGTTTGGA CAAACCACAA CTAGAATGCA GTGAAAAAAA TGCTTTATTT
  301 GTGAAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT AAGCTGCAAT AAACAAGTTA
  361 ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT
  421 AAAGCAAGTA AAACCTCCAC GTACCTTAAT ATTACTTACT TATCATGGTA GCTTGGGCTG
  481 GCGTAATAGC GAAGAGGCCC GCACCGATCG CCCTTCCCAA CAGTTGCGCA GCCTGAATGG
  541 CGAATGGCAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT
  601 CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA
  661 GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT
  721 GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC
  781 ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GNAACCCTAA
  841 AGGGANCCCC CGNNTTANAG CTTGACGGGG AAAGCCGGCG AACGTNGCGA GAAANGAAGG
  901 GAAGAAAGCG AAAGGANCGG GCNCTAGGNN GCTGGNAAGN GNANCGGNCA CGCNGCNCGN
961 ANNCNCNNNN CCCGNCNNNN NNNNNGNNNN NNTNCNNG
//
Primer: GV (Pr0748)
Region: Tcf motiff mtx7,IFN-b basal pro,Luc
Sequence file: RDB17506_A9Ibb.seq check
>02969_17506_A9Ib_pGV_Cw_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNCTN NNGCGTCCTT TGGCCCGCCC TTTGGCCCTA CCTTTGGCCG
   61 CGCCTTTGGC CCGCCCTTTG GCCCTACCTT TGGCCCCGCC TTTGGCCCTC GAGATCCGCT
  121 GAATAGAGAG AGGACCATCT CATATAAATA GGCCATACCC ATGGAGAAAG GACATTCTAA
  181 CTGCAACCTT TCGCAAGCTT GGCATTCCGG TACTGTTGGT AAAATGGAAG ACGCCAAAAA
  241 CATAAAGAAA GGCCCGGCGC CATTCTATCC TCTAGAGGAT GGAACCGCTG GAGAGCAACT
  301 GCATAAGGCT ATGAAGAGAT ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA
  361 TATCGAGGTG AACATCACGT ACGCGGAATA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC
  421 TATGAAACGA TATGGGCTGA ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT
  481 TCAATTCTTT ATGCCGGTGT TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA
  541 CGACATTTAT AATGAACGTG AATTGCTCAA CAGTATGAAC ATTTCGCAGC CTACCGTAGT
  601 GTTTGTTTCC AAAAAGGGGT TGCAAAAAAT TTTGAACGTG CAAAAAAAAT TACCAATAAT
  661 CCAGAAAATT ATTATCATGG ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC
  721 GTTCGTCACA TCTCATCTAC CTCCCGGTTT TAATGAATAC GATTTTGTAC CAGAGTCCTT
  781 TGATCGTGAC AAAACAATTG CACTGATAAT GAATTCCTCT GGATCTACTG NGTTACCNAA
  841 NGGTGTGGCC CTTCCGCATA GAANTGCCTG CGTCAGATTC TCGCANGCCN GANATNCTAT
  901 TTTTGGNAAT CAAATCATTC CNGANACTGC GATTTTNAGT GNNGNNCCNT TCCATCACGG
961 NTTTGGAATG NTTACTACNC TCNNNNNNTN GANNTGNNGN NNTNC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ueda, Y., Wnt/beta-catenin signaling suppresses apoptosis in low serum medium and induces morphologic change in rodent fibroblasts. Int. J. Cancer, 99 (5): 681-688 (2002). PMID 12115501. [link to RRC of NBRP]
user_report Tanaka, N., APC Mutations as a Potential Biomarker for Sensitivity to Tankyrase Inhibitors in Colorectal Cancer. Mol. Cancer Ther. 16 (4): 752-762 (2017). PMID 28179481. [link to RRC of NBRP]