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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

p3AP1 (PMA) RE-TK hRluc(F) (#RDB03452)

Reporter construct of AP1 response element/PMA

Alternative name 2055C129F
Clone info. 3' AP1 response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the 3' AP1 response element is 5'-GATCCTGACTAATGACTAATGACTAAG-3'.
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial AP1 response element/PMA synthesized
Depositor|Developer Yokoyama, Kazunari |
 
Remarks, protocol and/or map (pdf) RDB03452.pdf

Distribution information

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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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MTAに書く付加的使用条件：
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB03452 p3AP1 (PMA) RE-TK hRluc(F) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The p3AP1 (PMA) RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB03452).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13982_A6C7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: TKproR
Sequence file: RDB13982_A6C7a.seq check
>03452_13982_A6C7_TkproR_D05_11.ab1
    1 CCTAGGAAAG TGTTTCGTTC TTCCCCTCCC CCCGCGTCAG ACAAACCCTA ACCACCGCTT
   61 AAGCGGCCCC CGCGAGGTCC GAAGACTCAT TTAGATCCTT AGTCATTAGT CATTAGTCAG
  121 GATCTGTCGA GCCATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC
  181 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC
  241 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG
  301 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT
  361 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA
  421 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC
  481 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC
  541 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT
  601 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT
  661 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC
  721 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA
  781 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA
  841 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
  901 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
  961 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCCT
 1021 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGCTTT ACATCTGGGC TCCCAGTGCT
1081 GCCATGATAC GCGAGACTCA CCGCCTTCAC CGGCCTCCAG AATTTAATTC AAGAGC
//
Primer: pAxCALNL_F1
Sequence file: RDB13982_A6C7b.seq check
>03452_13982_A6C7_pAxCALNL_F1_E05_14.ab1
    1 ATAATATATC TTATCATGTC TGCTCGAGCG GCCGCTCTAG AATTACTGCT CGTTCTTCAG
   61 CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT CCTCCTGGCT
  121 GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG CTCCCTCGAC
  181 AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC ATCTTAGGCA GATCGTCGCT
  241 GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT TGCCTCCCTT
  301 AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC CCTTCTCCTT
  361 GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA TCTTGCTTGG
  421 GAGCATGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT CTTCGCTCTT
  481 GATCAGGGCG ATATCCTCCT CGATGTCAGG CCACTCGTCC CAGGACTCGA TCACGTCCAC
  541 GACACTCTCA GCATGGACGA TGGCCTTGAT CTTGTCTTGG TGCTCGTAGG AGTAGTGAAA
  601 GGCCAGACAA GCCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA GGTTCAGCAG
  661 CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC CATTCCCGCT
  721 CTTGCCGGAC TTACCCATTC CGATCAGATC AGGGATGATG CATCTAGCCA CGGGCTCGAT
  781 GTGAGGCACG ACGTGCCTCC ACAGGTAGCT GGAGGCAGCG TTACCATGCA GAAAAATCAC
  841 GGCGTTCTCG GCGTGCTTCT CGGAATCATA GTAGTTGATG AAAGGAGTCC AGCACGTTCA
  901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
  961 CGTAACACTT GGAAGCATGG TGGCTAGCCT AATAGTGAGT CGTATAGTAC TCTAGCCTTA
 1021 GAGCTGTATG GACCTGGGGA GTGACACTGT GGAAGAAGGC AAGTGGATGT CAGTAGACCA
1081 ATAGTTGCCT ATCAGAAACG GCAAAGAGAG TCC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Ogawa E., p63/p51-induced onset of keratinocyte differentiation via the c-Jun N-terminal kinase pathway is counteracted by keratinocyte growth factor. J. Biol. Chem., 283, 34241-34249 (2008). PMID 18849344. [link to RRC of NBRP]