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pKM2L-phHIF1A

Promoter Bank clone, Human hypoxia-inducible factor 1 alpha subunit (HIF1A) promoter

Catalog number RDB05523
Resource name pKM2L-phHIF1A
Alternative name p1938-16F
Clone info. PCR amplified human hypoxia-inducible factor 1 alpha subunit (HIF1A) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 61692498 to 61695674(NC_000014.9); relatively -2903 to +274, where +1 corresponds to 1 nt of NM_001530.3.
Comment PCR cloning, forward primer, 1938 F2: 5' tgcatttcaccttcaaaaggctca 3'; reverse primer, 1938 R2: 5' acccctcgtgagactagagagaag 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human hypoxia-inducible factor 1, alpha subunit genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human hypoxia-inducible factor 1, alpha subunit

          Reference sequence
            

          Distribution information

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          RDB05523 pKM2L-phHIF1A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB05523z.seq

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13521_A5Ipp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13521_A5Ipa.seq check
          >05523_13521_A5Ip_M13(-40)_A05_02.ab1
              1 AGGGCAAAGG CAAGGGTTGC ATGCCTGCAG GTCATTTAAA TAGGGATAAA CAGGGTAATG
             61 ATTAAGATCT CGAGCTCTAA GGGGCACGTA GGGCATGCAC TAGTGGCTGC ATTTCACCTT
            121 CAAAAGGCTC AATGCTCTGT ATTATTTAGT AGTAATCAAA ATTTCAAGTT TTACTTAACC
            181 TCCTGATTCA CTGCCCAATT TCCTAATAAA TACGGGCTAA GGGTCAATGG GGTCATTTGC
            241 AAGTAATCTT GTAGTCTACT CAGAAAGTTC TGCAAAGTTA GAAAGTGATT AAATGACTGT
            301 TTGTTAAGAT ATACTTACAT AGTAATAACC TAAATGCATT TGTTAAGTGG TTGTAGAGAG
            361 AGGGATTTAA AATTTTATCC TATATGAAAT TTTCCTTTTT GGTGTCTGTT ATTTAATAGG
            421 ATTGTTTGAA TTAGGGGATA CTATTTGGTG CCTTTGTAAC TATACGAAAA TTAGTTGGTT
            481 GAATATTACT GCTTTCCATG TTCATATTTA TATTTGTATA GACATATATA TATATACACA
            541 TATACTACTT TCCTTTCCAT TTTCATATTT ATATTTGTGT ATACACATAT ACATAAACAT
            601 ATATTTTATA CATTTTTGAA AAGGAAAATT AACTTAAGGG CATATTTAAT GAATATTCAA
            661 AAATTTTTTT GCTGATCAAA TTATCATTCT GCTTTAAACT TTTGAAATGA TCCAAAAAAA
            721 TTTTAAATGA CTTAGATTTA CTGTTACAAA ATGCTTGTCT TTTGATGTCA CAAACATTAT
            781 ATACTATAAT CACTGGCCAG AGAGAATTGC TATAAGTATA ATGAAAAGGG AAATGATGGA
            841 AGATCTCTGC AGCTATCCTC ATAAATGAGG GTGGGGAACA CGATGGGCAG TTCCAAAGTT
            901 GAAAATAGAG AATATATGTG GGATTTATAT TAACATAATT GGTATTCTTG GATAGTAAAA
            961 ATGGCTAAAA CTGTAGGAGA AAGCCCGAAG TAATTACTGG TTAACAGAGG GAATTAAATT
           1021 TTGAGGGGCA AATAATAATG ATGATAGGCC AGGGCACTGT GGGCTCATTG CCCTGAAAAT
           1081 CCCAGCACCT TTGGGGAACC GCGAAGGCGA GACCGGAACC ACCTTGAGGG TCAGGGAGGT
          1141 TCGAGGAGCA ACCCTGGGCA AACATTGGGT GAATAAACCT TCGCGTCTTC C
          //
          Primer: phRLR2
          Sequence file: RDB13521_A5Ipb.seq check
          >05523_13521_A5Ip_phRLR2_A11_02.ab1
              1 ACTTCCTGTC TCGACAGCCC AGTTTCTATT GGTCTCCTTA AACCTGTCTT GTAACCTTGA
             61 TACTTACCTG CCCAGTGCCT CACGACCAAC TTCTGCAGTA CCCGGGTGAA TTCTACGCGT
            121 CGACAGGCAC CCCTCGTGAG ACTAGAGAGA AGCGGGCGGC AATCGCCGGG GTCCGGGCCC
            181 GGCTCCGAGC CTCTCCTCAG GTGGCTTGTC AGGGCCCAGC CCGACACACT GGCCGAAGCG
            241 ACGAAGAGGG TGATCCTCCT GTCCCCTCAG ACGAGGCAGC ACTGTGCACT GAGGAGCTGA
            301 GGCAGCGTCA GGGGGCGGGC AAGGGCGGAG GCGCGCTCGG GCGCGCGGGG AGGGGAGAGG
          361 GGGC
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB13521_A5Ipc.seq check
          >05523_13521_A5Ip_pAxCALNL_F1_B11_05.ab1
              1 ACCAAGGAAA TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT
             61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG
            121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC
            181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC
            241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC
            301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC
            361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT
            421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT
            481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC
            541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG
            601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG
            661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC
            721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC
            781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT
            841 CACGGCGTTC TCGGCGTGCT TCTCGGAATC ATAGTAGTTG ATGAAAGGAG TCCAGCACGT
            901 TCATTTGCTT GCAGCGAGCC CACCACTGAG CCCAGTGATC ATGCGTTTGC GTTGCTCGGG
            961 GTCGTACACC TTGGAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
           1021 TTTAGAGCTG TAATTGAACT GGGGAGTGGA CACCTGTGAG AGAAGGCAAG TGATGTCAGT
           1081 AGACATAGGT GCCTATCAGA AACCGCAGAG TCCTTCTCTG TCTCCGACAA GCAGTTCTAA
          1141 TTGCTTCTCT AACCTGTTC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Minet, E., HIF1A gene transcription is dependent on a core promoter sequence encompassing activating and inhibiting sequences located upstream from the transcription initiation site and cis elements located within the 5'UTR. Biochem. Biophys. Res. Commun. 261 (2): 534-540 (1999). PMID 10425220.

          2022.05.18

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