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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phIGNA2

Promoter Bank clone, Human integrin alpha 2 subunit (ITGA2) promoter

Catalog number RDB05527
Resource name pKM2L-phIGNA2
Alternative name p1942-2F
Clone info. PCR amplified human integrin alpha 2 subunit (ITGA2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 52988380 to 52989468(NC_000005.10); relatively -946 to +143, where +1 corresponds to 1 nt of NM_002203.3.
Comment PCR cloning, forward primer, F9PB0070F: 5' ggtttcagcaatgtgctggaaatttg 3'; reverse primer, F9PB0070R: 5' cctgggtctgaccgggggagcgt 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human integrin, alpha 2 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human integrin, alpha 2
          Reference sequence
            AF512556, AC008966

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05527 pKM2L-phIGNA2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12780_A4Dp.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB12780_A4Dpa.seq check
          >05527_12780_A4Dp_M13(-40)_A08_02.ab1
              1 AAGCCGGTGC AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA
             61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCGGTTTCAG CAATGTGCTG
            121 GAAATTTGTG GCCAAACTTT TGGATAAAAC ATAAGATTCA GACAAGAAAA ATTAATATCC
            181 TATTTCTTAG GTTTTTAACT AAAAATCTCG GATATAGAAT GTTCTCTTTC CAGATGTGAG
            241 ACTGAAATAA TGGAAGAAAA TGGTCACAGT CTATGTCACG GTGTAATGTA GACAGGGAGA
            301 GTCACAAGTG CATGTATGGG CTCTCAGCCT TTTCTCTGGC CTTGAAGTTA ATCTCCTAAA
            361 CTCAGTCATT AAATCAGCTC TTAATTATCT CGGAGGAGAG GAAGTAATGA TGATTTCTCT
            421 CTCTCTGTCT CTCTGTCACA CACACACACA CACACACAGC TCTTGCAGCA GGTATTGCTT
            481 AAATATCACC TTGGATAATC ATAACTTGTG AGCAGATCTT CTTTCCTGAT GAGTTAGTTT
            541 CTAACCTGGT CATTCTGCGC TTATTTTTGT CCCTTTCTCC ACCCACTTAG GAAAAACAGA
            601 GAAAGGGACG CACCGCGCAG CCCCTAGGCA CTGTGGTTTA GGGCTAGTGC CCTCGGCACC
            661 CGCTGCCAGG AGCCGGGCGC TGCCAAGGGC TGCGGAGGGG CCACGTTCTC CCGGGGACTG
            721 GGGCATCTCC TGCGTGCTGG CGACAGGCTG CGGGGGCGGA GTGGTGCCAG GGCGGGCGCT
            781 CGCCCGTCGG ATATGCCCAC CCGTCCCGTC CAGGGCAGGA AAGCCTGCCA GGGCGCCATC
            841 CCCATCCCCA CCGCCTCCAG GCTGCCGGGG CTGGGCCGCT GTACGGGAGC CAAGGTGCGG
            901 TGCCCCGCGT GTGGACGAGC CGAGGTGCAG CCCGCGGGGC CGCAGGGCCG GGGTGGGGCG
            961 GGGCGCGGCC GGAGCAGATC CGGTGTTTGC GGAATCAGGA GGGGCGGGCC GGGGCGGGCC
           1021 CTCGGCGCTG CAGGAGCTGC CCAGAAACTT TTCCCTGCTC TCACGGGCGG GGGAGAGAGC
           1081 CCTCTGGACA GCTTCTAGAG TGTGCAGTTC TCGTATCCTC GGCAGGTATC TCTGCACTCT
           1141 GCACAGGCAC TACCGTTCCC CCGGTCGGAC CAGGTCTGTT CGACGCGTAG AAATTTCATC
          1201 G
          //
          Primer: phRLR2
          Sequence file: RDB12780_A4Dpb.seq check
          >05527_12780_A4Dp_phRLR2_B08_05.ab1
              1 ACCTTCTTTC CGTCTCGGAA AGCCCAGTTT CTATTGGTCT CCTTAAACCT GTCTTGTAAC
             61 CTTGATACTT ACCTGCCCAG TGCCTCACGA CCAACTTCTG CAGTACCCGG GTGAATTCTA
            121 CGCGTCGACA GGCCCTGGGT CTGACGGGGG GACGGTAGTT GCGCTGGGTT TGCAGAGGTT
            181 TGCAGAGGAT ACCCTTGGCC GAGGGATACG AGAACCTGCA CACTCTAGAA GCTGTCCAGA
            241 GGGCTTCTCT CCCCCGCCCG GTGAGAGCAG GGAAAAGTTT CTGGGCAGCT CCTGCAGCGC
            301 CGAGGGCCCG CCCCGGCCCG CCCCTCCTGA TTCCGCAAAC ACCGGATCTG CTCCGGCCGC
            361 GCCCCGCCCC ACCCCGGCCC TGCGGCCCCG CGGGCTGCAC CTCGGCTCGT CCACACGCGG
            421 GGCACCGCAC CTTGGCTCCC GTACAGCGGC CCAGCCCCGG CAGCCTGGAG GCGGTGGGGA
            481 TGGGGATGGC GCCCTGGCAG GCTTTCCTGC CCTGGACGGG ACGGGTGGGC ATATCCGACG
            541 GGCGAGCGCC CGCCCTGGCA CCACTCCGCC CCCGCAGCCT GTCGCCAGCA CGCAGGAGAT
            601 GCCCCAGTCC CCGGGAGAAC GTGGCCCCTC CGCAGCCCTT GGCAGCGCCC GGCTCCTGGC
            661 AGCGGGTGCC GAGGGCACTA GCCCTAAACC ACAGTGCCTA GGGGCTGCGC GGTGCGTCCC
            721 TTTCTCTGTT TTTCCTAAGT GGGTGGAGAA AGGGACAAAA ATAAGCGCAG AATGACCAGG
            781 TTAGAAACTA ACTCATCAGG AAAGAAGATC TGCTCACAAG TTATGATTAT CCAAGGTGAT
            841 ATTTAAGCAA TACCTGCTGC AAGAGCTGTG TGTGTGTGTG TGTGTGTGAC AGAGAGACAG
            901 AGAGAGAGAA ATCATCATTA CTTCCTCTCC TCCGAGATAA TTAAGAGCTG ATTTAATGAC
            961 TGAGTTTAGG AGATTAACTT CAAGGCCAGA GAAAAGGCTG AGAGCCCATA CATGCCACTT
           1021 GTGACTCTCC CTGTCTACAT TACACCGTGA CATAGACTGT GACCATTTTC TTCCATTATT
           1081 TCAGTCTCAC ATCTGGAAAG AAGAACATTC TATATCCGAG ATTTTTAGTA AAACCCTAAG
           1141 AAAATAGGAT ATATTTTCTT GTCTGAATCC TAATGTTTTA TCAAGTTGTC CACAAATTTC
          1201 CAGCCACACA TGTTGCCC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl