RDB05534 - pKM2L-phTERT

pKM2L-phTERT (#RDB05534)

Promoter Bank clone, Human telomerase reverse transcriptase, catalytic subunit (TERT) promoter

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Remarks, protocol and/or map (pdf)	RDB05534.pdf
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Alternative name	p1951-6F
Clone info.	PCR amplified human telomerase reverse transcriptase, catalytic subunit (TERT) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 1296442 to 1294990(NC_000005.10); relatively -1395 to +58, where +1 corresponds to 1 nt of NM_198253.2.
Comment	PCR cloning, forward primer, G10PB0079F: 5' agagacaattcacaaacacagccct 3'; reverse primer, G10PB0079R: 5' cgcgggggtggccggggccagg 3' Entire sequence of promoter region has not been confirmed.
Vector backbone	pKM2L (RDB04026) (Plasmid)
Size of vector backbone	4.2 kb
Selectable markers	Kan^r
Growth conditions	37C, LB+Kan
Gene/insert name	Human telomerase reverse transcriptase, catalytic subunit (TERT) genomic DNA
Depositor\|Developer	DNA Bank, \|

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Catalog #	Resource name	Shipping form	Fee
RDB05534	pKM2L-phTERT	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge