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pKM2L-phUPA

Promoter Bank clone, Human urokinase plasminogen activtor (uPA) promoter

Catalog number RDB05547
Resource name pKM2L-phUPA
Alternative name p1966-9F
Clone info. PCR amplified human urokinase plasminogen activtor (uPA) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 57961 to 60063 of AL596247.22 corresponding -2068 to +35 (the transcription start site is designated +1. Fig. 1 of Verde, P. Nucleic Acids Res. 16 (22): 10699-10716, 1988) is cloned. Alternatively, 73909061 to 73911163(NC_000010.11); relatively -2071 to +32, where +1 corresponds to 1 nt of NM_002658.5 was cloned.
Comment PCR cloning, forward primer, 1966 F2: 5' gtgctaggtgaaaggtggcacgggg 3'; reverse primer, 1966 R2: 5' cccgggctccggggctgcggtctcc 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human urokinase plasminogen activtor (uPA) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human urokinase plasminogen activtor (uPA)
          Reference sequence
            AL596247
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。　2. 謝辞の表明を必要とする。
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          RDB05547 pKM2L-phUPA Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pKM2L-phUPA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05547).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          reference Verde, P., An upstream enhancer and a negative element in the 5' flanking region of the human urokinase plasminogen activator gene. Nucleic Acids Res. 16 (22): 10699-10716 (1988). PMID 3205721.
          user_report Shimizu, M., Plasminogen activator uPA is a direct transcriptional target of the JAG1-Notch receptor signaling pathway in breast cancer. Cancer Res., 71, 277-286 (2011) . PMID 21199807.

          2023.05.02

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