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pKM2L-phCDKN2(2) (#RDB05811)

Promoter Bank clone, Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) promoter


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)
GenBank Flat File Format open
SnapGene file download
Publication Unpublished bioresource
Test sheet Data Sheet open 
 
Alternative name p2002-2F
Clone info. PCR amplified human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic DNA (21,995,329 - 21,994,353nt of NC_000009.12) corresponding to 1 to 91nt of NM_000077.4 and 0.9 kb up-stream was cloned.
Comment PCR cloning, forward primer, 2002 F4: 5' ctatgcctcaaaacaagattgcaag 3'; reverse primer, 2002 R4: 5' ctattaactccgagcacttagcga 3'. (Mol. Cell. Biol., 16, 859-867 (1996)).
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Growth conditions 37C, LB+Kan
Gene/insert name Human p16 cyclin-dependent kinase inhibitor 2 (CDKN2/INK4A/CDK4I/MTS1) genomic DNA
Depositor|Developer DNA Bank, |

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Catalog # Resource name Shipping form Fee
RDB05811 pKM2L-phCDKN2(2) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge


How to cite this biological resource

Materials & Methods section:

The pKM2L-phCDKN2(2) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05811).

Reference section:

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles