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pKM2L-phSMAD7

Promoter Bank clone, Human Smad7 promoter

Catalog number RDB05888
Resource name pKM2L-phSMAD7
Alternative name p1953-8F
Clone info. PCR amplified human Smad7 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). This clone has genomic sequence (48,951,746 to 48,955,347nt of NC_000018.10) corresponding -3.3 kb to +270 bp (tanscription start site P1 is designated +1. Fig. 2 of Denissova, N.G. et al. Proc. Natl. Acad. Sci. USA 97: 6397-6402, 2000). Note that this clone does not contain any part of cDNA sequence of NM_005904.3, a representative sequence of human SMAD7. Alternatively, 48955347 to 48951747(NC_000018.10); relatively -4636 to -1035, where +1 corresponds to 1 nt of NM_005904.3 was cloned. (reference: Proc. Natl. Acad. Sci. U.S.A., 97, 6397-6402, 2000, PMID:10823886; J. Biol. Chem., 275, 29308-2917, 2000, PMID:10887185)
Comment PCR cloning, forward primer, 1953 F4: 5' gtgtgctccggcctctcattacag 3'; reverse primer, 1953 R4: 5' tcctcctccgagactctcctcgt 3' Known differences (at least): C insertion between 48,952,117 and 48,952,118nt. G to A substitution corresponding to 48,951,914nt of NC_000018.10. Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human Smad7 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human Smad7
          Reference sequence
            AC114684

          Distribution information

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          RDB05888 pKM2L-phSMAD7 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05888_A9Cfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05888_A9Cfa.seq check
          >05888_05888_A9Cf_M13_-40_E01_05_ABI08.ab1
              1 AAGCAGTTCA AGCTTGCATG CCTGCAGGTC ATTTAAATAG GGATAACAGG GTAATGATTA
             61 AGATCTCGAG CTCTAAGCTT CACATATGCA TGCACTAGTG GCGTGTGCTC CGGCCTCTCA
            121 TTACAGAGCC TTAGCACTAC TGGCCAGATC TCCAAAAGAT TCTTCAGATT CTCCATTTAG
            181 CCAAGAAACT GAATTAGAAT TATGACCTCT ACTTTCTCTG CTTATTTAAG TCCTTTCTGT
            241 TGCTATGAAG CCTGGTCTGA CCACTCTCAA TAAGTGAAGA TCTTTTAGGG AGATCCAGAA
            301 CAGAGAGGGA ATGGGACCCT AGAGAGCGGA AACTTGCCCA GAAAATCTTA TAAACTCTGC
            361 CAAGTGCTCG CCTAACGTGG GGATTGCTGT TGGCCCTAGT TGCTTAACCA GTGAGCGGCA
            421 ACGCCCCAGC CTGGAATCAG GTTTTCTGAC CTCTAGTCTG GTACTCTTTC TAAGCCTTTC
            481 TAAGGTGAAA ATATGATACA TTTCCCAGAC ACCTTAAAAA TCCCATTTCT GGGCCGGGCG
            541 CTGTGGCTCA TGCCTGTTAT CCCACACTTT GGGAGGCTGA GGCGGGTGGA TCGCCTGAGC
            601 TCAGGAGTTC GAGACTAGCC TGGGCAACAC GGTGAAACCT TGTCTCTGCT AAAATACAAA
            661 AAAATTAGCG AGGTGTGGAG TGCACGTGTA GTTCCAGCTA CTAGGGAGGC TGAGGCAGGA
            721 GAATTGCTTG AACCCAGGAG GCGGAGGTTG CAGTGAGCTG AGATCATGCC ACTGCCCTCT
            781 AGGCTGGGTG ACAAAGCAAG GCTCCATCTC AAAAAAAAAA AAAAAAAAAA TCCCACTTCT
            841 GTCTCTTTCG TCTTCCCTTG CCTATCATGA TAGGCCTCCC AAAGTGCTGG GATTACACTC
            901 CTTCTCCATT CTCTCCTGTG CTTTCTCAGC TGCTGTCTTC ACAGGTTGCT TCTTAGAATG
            961 TTTTTTTTAA TACACACACA AGGTTTCAAT GCCCTTTTAT ACAAGCCATA TTTTAATTTA
           1021 CACAAAATTA TGATGTTATA CACTGCAGCT TATGGTGGCC TTTACACAGT AAATGTTCAG
           1081 ATTGCAGCTT CTGTCGTCAG TAGGATCTGC ACTGCTGCAT CTACTATCCT TCAAGGTCAT
          1141 AAAGGTGTTT AAGGAATTCT TGCT
          //
          Primer: phRLR2primer
          Sequence file: RDB05888_A9Cfb.seq check
          >05888_05888_A9Cf_phRLR2primer_F01_06_ABI08.ab1
              1 ATTCTTTGTC TCGAAAGCCC AGTTTCTATT GGTCTCCTTA AACCTGTCTT GTAACCTTGA
             61 TACTTACCTG CCCAGTGCCT CACGACCAAC TTCTGCAGTA CCCGGGTGAA TTCTACGCGT
            121 CGACAGGCTC CTCCTCCGAG ACTCTCCTCG TCGCGCCCGG GAGCCTCCTT GTCCCCGGTC
            181 CGCCCTCTCC TGGCGCTCCG GTCCTTCTGC CGCCGCCAGG GGCTCGCCGC GCCGCACTCA
            241 GGGGCTCAGG GGCCGGGCGC TCGGCGGCTC GGCGCCGACG GACTGGCTCT GTCTCAGGCA
            301 GCTCTCTCCC GCGCGGCGAG CGGACCGAGC ACGGCGCCCG GCTGGCTCGG CTGGCGCGGC
            361 TCGGGGACAG GATCTTCCGT GCGCCGAGCA GCAAGCGAGT GTGCCCGGGG CTCACCGCCT
            421 CCCGCAAGGC CTCCCGCCCC CGCCCCCTTC CCTCCCCCTT CCTCCCCCTT CCCTCCCCCG
            481 CCCCCAGCCG CCGCAGCCGC CGCCGCCTCC TCCCCGCCCC CCCGCACCCC CCCTCCGGCC
            541 CTCTGCTCGG CTGGTTCCAC TGCGCAGTGG CGCGCCCGGC TCCGGCCTCG TCACGTGGCC
            601 GTCTAGACAC CCTGTCGCTT TAAAAAAAAA AAAAGCGATT GTGTTTCGCA AACAACAGAT
            661 CGGGTTTCTA AAAGCTATTT CTCCCCCCAA CCCCCCGCCA CCGCCACCCC CTCCCGGGTC
            721 TGTAGAGGGG GTACCGATGG AGGGGAGAGA GATAGGTGGG GGGCAGAGAA GCTCCCAGAA
            781 TGGATTGAGC CCCGGCCGGA GCCATGGAGA AATTGGGAAA AGCAGGAGCA CCGAGGCGGG
            841 CTTCGCCGCG AGTTTTGAGC TGAGCGAGCG GGTCGTGGCC CGATTTCGAC CGGCTGGGTT
            901 TCCGCGGTGC ATCTCGCGCG GGTCGGCTTA GCGCGCTCAT TCATGGATTA TGTTAAAGCC
            961 CTGCGGTGAT CCTTGCCCGC CCCGAGGCAG GGAGGAAGCG CTGATCCGGT AAGCTTACGG
          1021 ACTGACTAGG CTGGAAGTGC CTTAGGACAT CG
          //
          Primer: pAxCALNLF1
          Sequence file: RDB05888_A9Cfc.seq check
          >05888_05888_A9Cf_pAxCALNLF1_G01_07_ABI08.ab1
              1 TACAAATGTA TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGAATTACT GCTCGTTCTT
             61 CAGCACGCGC TCCACGAAGC TCTTGATGTA CTTACCCATT TCATCTGGAG CGTCCTCCTG
            121 GCTGAAGTGG AGGCCCTTCA CCTTCACGAA CTCGGTGTTA GGGAACTTCT TAGCTCCCTC
            181 GACAATAGCG TTGGAAAAGA ACCCAGGGTC GGACTCGATG AACATCTTAG GCAGATCGTC
            241 GCTGGCCCGA AGGTAGGCGT TGTAGTTGCG GACAATCTGG ACGACGTCGG GCTTGCCTCC
            301 CTTAACGAGA GGGATCTCGC GAGGCCAGGA GAGGGTAGGC CGTCTAACCT CGCCCTTCTC
            361 CTTGAATGGC TCCAGGTAGG CAGCGAACTC CTCAGGCTCC AGTTTCCGCA TGATCTTGCT
            421 TGGGAGCATG GTCTCGACGA AGAAGTTATT CTCAAGCACC ATTTTCTCGC CCTCTTCGCT
            481 CTTGATCAGG GCGATATCCT CCTCGATGTC AGGCCACTCG TCCCAGGACT CGATCACGTC
            541 CACGACACTC TCAGCATGGA CGATGGCCTT GATCTTGTCT TGGTGCTCGT AGGAGTAGTG
            601 AAAGGCCAGA CAAGCCCCCC AGTCGTGGCC CACAAAGATG ATTTTCTTTG GAAGGTTCAG
            661 CAGCTCGAAC CAAGCGGTGA GGTACTTGTA GTGATCCAGG AGGCGATATG AGCCATTCCC
            721 GCTCTTGCCG GACTTACCCA TTCCGATCAG ATCAGGGATG ATGCATCTAG CCACGGGCTC
            781 GATGTGAGGC ACGACGTGCC TCCACAGGTA GCTGGAGGCA GCGTTACCAT GCAGAAAAAT
            841 CACGGCGTTC TCGGCGTGCT TCTCGGAATC ATAGTAGTTG ATGAAGGAGT CCAGCACGTT
            901 CATTTGCTTG CAGCGAGCCC ACCACTGAGG CCCAGTGATC ATGCGTTTGC GTTGCTCGGG
            961 GGTCGTACAC CCTTGGAAGC CATGGTGGCT AGCCTATAGT GAGTCGTATT AAGTACTCTA
           1021 GCCTTTAAGA GCTGTAATTT GAACTGGGAG TGGACACCTG TGGAGAAGAA AGGCAAAGTG
           1081 GAATGTCAGT AAGAACCAAT AGGTGCCTAT CAGAACGGCA GAGTCCTTCT CTGTCTCGAT
          1141 AGCCCCAGAT TTTCTAGTAT TG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl