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pKM2L-phPTP1B(2)

Promoter Bank clone, Human protein tyrosine phosphatase 1B (PTP1B) promoter

Catalog number RDB05909
Resource name pKM2L-phPTP1B(2)
Alternative name p1995-1F
Clone info. PCR amplified human protein tyrosine phosphatase 1B (PTP1B) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 50508470 to 50510435(NC_000020.11); relatively -1851 to +115, where +1 corresponds to 1 nt of NM_002827.3.
Comment PCR cloning, forward primer, 1995 F2: 5' tgcaggaaggcaacctgaccaacct 3'; reverse primer, 1995 R2: 5' gcaatccccgacccctgcagctac 3' (reference: J. Biol. Chem., 276, 25512-25519, 2001, PMID:11316810) Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human protein tyrosine phosphatase 1B (PTP1B) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human protein tyrosine phosphatase 1B (PTP1B)

          Reference sequence
            AL034429

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 1. 本件リソースは学術研究機関にのみ提供し、学術研究にのみ利用することができる。 2. 謝辞の表明を必要とする。
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          RDB05909 pKM2L-phPTP1B(2) Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl