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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-CA-Venus (#RDB05965)

Lentivirus vector plasmid of Venus fluorescent protein driven by CAG promoter.

Clone info. Lentivirus vector plasmid of Venus fluorescent protein driven by CAG promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CS-CA-MCS (RDB05963) (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions 37C, LB+Amp
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyoshi, Hiroyuki |
 
Sequence (full) RDB05965zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf) RDB05965.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB05965 CS-CA-Venus DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-CA-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05965).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16933_A9Blp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAG pro,Venus 5'
Sequence file: RDB16933_A9Bla.seq check
>05965_16933_B2BfF_CAG_R1_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNNNNNN GGCGCGGGGC GNAGCGGTGC GGCGCCGGCA
   61 GGAAGGAAAT GGGCGGGGAG GGCCTTCGTG CGTCGCCGCG CCGCCGTCCC CTTCTCCCTC
  121 TCCAGCCTCG GGGCTGTCCG CGGGGGGACG GCTGCCTTCG GGGGGGACGG GGCAGGGCGG
  181 GGTTCGGCTT CTGGCGTGTG ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT
  241 TCTTCTTTTT CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG
  301 GCAAAGAATT GATTTGATAC GTACGGTTGG GCTGCAGGAA TTGATCCACC ATGGTGAGCA
  361 AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA
  421 ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA
  481 CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA
  541 CCCTGGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT
  601 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG
  661 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA
  721 TCGAGCTGAA GGGCATCGAC TTCNAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT
  781 ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGANNA GCAGAAGANC GGCATCAAGG
  841 CCNACTTCNA GATCCGCCAC AACATCGNNG ANGGCGGCGT GCAGCTCGCC NANNNCTACC
  901 NGCNNANCNC CNNCNTCGNC GANNGNCCCN NGCNGCTGNC CGANAACCAC TANCTGANCT
  961 ANCNNTCCNN CCTNANCNAA GANCCNNACG AGAANCNNGN TNNNNTNGNN NNNGNTNGAN
1021 TTCNNNNNNN NNN
//
Primer: PRE_rev (Pr0250)
Region: PRE,Venus 3'
Sequence file: RDB16933_A9Blb.seq check
>05965_16933_B2BfF_PRE_rev_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NANNNNGAGC AACATAGTTN NNANTACCNG TCAATCTTTC
   61 ACAAATTTTG TAATCCAGAG GTTGATTATC GATAAGCTTG ATCCCTCGAG AGGCCTTGAA
  121 TTCTTACTTG TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG
  181 CAGGACCATG TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGGGCGGACT GGTAGCTCAG
  241 GTAGTGGTTG TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG
  301 GTCGGCGAGC TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT
  361 GCCGTTCTTC TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG
  421 CTTGTGCCCC AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT
  481 CACCAGGGTG TCGCCCTCGA ACTTCACCTC GGCGCGGGTC TTGTAGTTGC CGTCGTCCTT
  541 GAAGAAGATG GTGCGCTCCT GGACGTAGCC TTCGGGCATG GCGGACTTGA AGAAGTCGTG
  601 CTGCTTCATG TGGTCGGGGT AGCGGGCGAA GCACTGCAGG CCGTAGCCCA GGGTGGTCAC
  661 GAGGGTGGGC CAGGGCACGG GCAGCTTGCC GGTGGTGCAG ATCAGCTTCA GGGTCAGCTT
  721 GCCGTANGTG GCATCGCCCT CGCCCTCGCC GGACACGCTG AACTTGTGGN CGTTTACGTC
  781 GCCGTCCAGC TCGACNAGGA TGGGCACCAC CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC
  841 CATGGTGGNT CNATTCCTGC AGCCCNACCG TACGTATCNA ATCNATTCTT TGCCNAAATG
  901 ATGANACAGC ACAATNNCCN GCACGTNGNC NNNGANCTGT ANGAAAAANA ANAANGNATG
  961 AANNTNGTTN GCAGANGNTC TANANCCGNC NNNNNCNNNN NNNANNNCNG NNNCNCGCCN
1021 NNNCCNNNNC CCNNCCNNNN NNNNNN
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,5'LTR,HIV-1 psi
Sequence file: RDB16933_A9Blc.seq check
>05965_16933_A9Bl_CMV_Forward_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNCCCNNT TGNNGCNNNN GGGCGGNAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
  121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
  181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
  241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
  301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
  361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
  421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT
  481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG
  541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA
  601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA
  661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA
  721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC
  781 CGCTGATCTT CAGACCTGGA GGAGGAGATA TGANGGANAA TTGGAGAAGT GANTTATATA
  841 AATATAAAGT AGTAAAAATT GNNCCNTTAN GAGTANCACC CACCNAGGCA AAGAGAAGAG
  901 TGGTGCAGAG AGAAAAAAGA NCANNGGGAN TAGGAGCTTT GTTCCNTGGG TTCTTGGGAN
961 CNNCNNNNGC ANTATGGGGC GCNNNNNCNA TGNNNCTGNN GGNANNNNNN NNNN
//
Primer: RRE_F1 (Pr0796)
Region: RRE
Sequence file: RDB16933_A9Bld.seq check
>05965_16933_B2BfF_RRE_F1_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNCNNNN NNNNNNNNTG CTGANGGCTA TTGAGGCGCA ACAGCATCTG
   61 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA
  121 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC
  181 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC
  241 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA
  301 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA
  361 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC
  421 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG
  481 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG
  541 GGACCCGACA GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC
  601 ATTCGATTAN TGAACGGNTC TACNAATGGC AGTATTCATC CNCNATTTTA AAAGAAAAGG
  661 GGGGNNTGGG GGGGNACAGN GCNNGGGNNN NNAGTAGACN TNANNNCNNN NGANNTNNNA
721 NCTNANNNAT TNCNAANNNA NNNNNNNAAA ANNNNNAANN TTTNNN
//
Primer: env_F (Pr0258)
Region: cPPT_CTS,CAG pro
Sequence file: RDB16933_A9Ble.seq check
>05965_16993_A9Bl_1_env_F_D10_10_ABI24.ab1
    1 CTACNAATGG CAGTATTCAT CCNCAATTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT
   61 GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA
  121 CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAAA TTCACTTTGA
  181 ATTGGGCTGC AGGAATCTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC
  241 CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC
  301 AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG
  361 ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT
  421 CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC
  481 TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA
  541 TTAGTCATCG CTATTACCAT GGTCGAGGTG AGCCCCACGT TCTGCTTCAC TCTCCCCATC
  601 TCCCCCCCCT CCCCACCCCC AATTTTGTAT TTATTTATTT TTTAATTATT TTGTGCAGCG
661 ATGGGGGCGG GGGGGGGGGG G
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,3'LTR
Sequence file: RDB16933_A9Blf.seq check
>05965_16933_B2BfF_bGH_rev3_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG ATNGGCTGGC AACTAGAAGG
   61 CACAGTCGAG GCTGATCNNC GGGTTTAAAC GGGCCCTGCT AGAGATTTTC CACACTGACT
  121 AAAAGGGTCT GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT
  181 GAAGCACTCA AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA
  241 GCTCCCAGGC TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGTCCGGA TGCAGCTCTC
  301 GGGCCATGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAATACT TCTCTCTCCG
  361 GGTCATCCAT CCCATGCAGG CTCACAGGGT GTAACAAGCG GGTGTTCTCT CCTTCATTGG
  421 CTTCTTCTAC CTTCTCTTGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG
  481 GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT
  541 GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC
  601 CCCCCTTTTC NTTTAAAAAG TGGCTAANAN CTACAGCTGC CTTGTAAGTC ATTGGTCTTA
  661 AANGNACCTG NNNGTGANTG GAAAANCCNN CTCCTCCTCC TCTNGNGCTT CNNNCCNGGC
721 NCNATCAGCN NNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,Zeo,SV40 pA
Sequence file: RDB16933_A9Blg.seq check
>05965_16993_A9Bl_1_SV40pro_F_E10_13_ABI24.ab1
    1 GTGAGGANGN TTTTTTGGAG GNCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC
   61 CATTTTCGGA TCTGATCAGC ACGTGTTGAC AATTAATCAT CGGCATAGTA TATCGGCATA
  121 GTATAATACG ACAAGGTGAG GAACTAAACC ATGGCCAAGT TGACCAGTGC CGTTCCGGTG
  181 CTCACCGCGC GCGACGTCGC CGGAGCGGTC GAGTTCTGGA CCGACCGGCT CGGGTTCTCC
  241 CGGGACTTCG TGGAGGACGA CTTCGCCGGT GTGGTCCGGG ACGACGTGAC CCTGTTCATC
  301 AGCGCGGTCC AGGACCAGGT GGTGCCGGAC AACACCCTGG CCTGGGTGTG GGTGCGCGGC
  361 CTGGACGAGC TGTACGCCGA GTGGTCGGAG GTCGTGTCCA CGAACTTCCG GGACGCCTCC
  421 GGGCCGGCCA TGACCGAGAT CGGCGAGCAG CCGTGGGGGC GGGAGTTCGC CCTGCGCGAC
  481 CCGGCCGGCA ACTGCGTGCA CTTCGTGGCC GAGGAGCAGG ACTGACACGT GCTACGAGAT
  541 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC
  601 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG
  661 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA
  721 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT
  781 GTCTGTATAC CGTCGANCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT
  841 GTGTGAAATT GTTATCCGCT CACNATTCCA CACNACATAC GAGCCGGAAG CATNAAGTGT
  901 AAAGNCNGGG GNGNCNNATG ANTGANCTAN NTCANNTTAA TTGCNTTGCN CTCACTGCCC
961 NCTTTNCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Nakajima, R., A noncoding RNA containing a SINE-B1 motif associates with meiotic metaphase chromatin and has an indispensable function during spermatogenesis. PLoS One 12 (6): e0179585 (2017). PMID 28658256. [link to RRC of NBRP]