Distribution of this resource is to be determined.
Clone info. | Thermus thermophilus expression plasmid. |
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Vector backbone | pET-11a, pET-11b, pET-3a (Plasmid) |
Selectable markers | Amp^r |
Gene/insert name | Thermus thermophilus genomic DNA |
Depositor|Developer | Kuramitsu, Seiki | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Thermus thermophilus expression plasmid.[link] |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Nature Struct. Biol., 7, 943-945) |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「Thermus thermophilus expression plasmid[link]」をご覧ください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nature Struct. Biol., 7, 943-945)。 |
Original, user report and related articles
original | Agari, Y., Structure and function of a TetR family transcriptional regulator, SbtR, from thermus thermophilus HB8. Proteins, 81 (7): 1166-1178 (2013). PMID 23408580. [link to RRC of NBRP] |
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original | Yokoyama, S., Structural genomics projects in Japan. Nature Struct. Biol., 7, 943-945 (2000). PMID 11103994. [link to RRC of NBRP] |
reference | Brkic, A., Antibiotic hyper-resistance in a class I aminoacyl-tRNA synthetase with altered active site signature motif. bioRxiv 2023.01.31.526100. |
reference | Morita, R., Molecular mechanisms of the whole DNA repair system: a comparison of bacterial and eukaryotic systems. J. Nucleic Acids., 2010: 179594 (2010). PMID 20981145. |
user_report | Mendoza, J., Structure of full-length cobalamin-dependent methionine synthase and cofactor loading captured in crystallo. Nat. Commun. 14 (1): 6365 (2023). PMID 37821448. [link to RRC of NBRP] |
user_report | Nakatani, M., Crystal structure of a nucleotide-binding domain of fatty acid kinase FakA from Thermus thermophilus HB8. J. Struct. Biol. 214 (4): 107904 (2022). PMID 36228973. [link to RRC of NBRP] |
user_report | Mega, R., The Crystal Structures of Thermus Thermophilus CMP Kinase Complexed With a Phosphoryl Group Acceptor and Donor. PLoS One 15 (5): e0233689 (2020). PMID 32469932. [link to RRC of NBRP] |
user_report | Ihling, N., Online measurement of the respiratory activity in shake flasks enables the identification of cultivation phases and patterns indicating recombinant protein production in various E. coli host strains. Biotechnol. Prog. 34 (2): 315-327 (2018). PMID 29314728. [link to RRC of NBRP] |
user_report | Hartwick, E.W., Ribosome-induced RNA conformational changes in a viral 3'-UTR sense and regulate translation levels. Nat. Commun. 9 (1): 5074 (2018). PMID 30498211. [link to RRC of NBRP] |
user_report | Taniguchi, H., A Key Enzyme of the NAD+ Salvage Pathway in Thermus thermophilus: Characterization of Nicotinamidase and the Impact of Its Gene Deletion at High Temperatures. J. Bacteriol. 199 (17). pii: e00359-17 (2017). PMID 28630126. [link to RRC of NBRP] |
user_report | Tajima, K., In vitro synthesis of polyhydroxyalkanoates using thermostable acetyl-CoA synthetase, CoA transferase, and PHA synthase from thermotorelant bacteria. J. Biosci. Bioeng. pii: S1389-1723 (16) 30121-30129 (2016). PMID 27342638. [link to RRC of NBRP] |
user_report | Tomoike, F., A putative adenosine kinase family protein possesses adenosine diphosphatase activity. Biosci. Biotechnol. Biochem. 80 (11): 2138-2143 (2016). PMID 27484886. [link to RRC of NBRP] |
user_report | Diederichs, S., High-level production of (5S)-hydroxyhexane-2-one by two thermostable oxidoreductases in a whole-cell catalytic approach J. Mol. Catalysis B: Enzymatic 121: 37–44 (2015) [link to RRC of NBRP] |
user_report | Miyazaki, T., Crystal structure and substrate-binding mode of GH63 mannosylglycerate hydrolase from Thermus thermophilus HB8. J. Struct. Biol. 190 (1): 21-30 (2015). PMID 25712767. [link to RRC of NBRP] |
user_report | Kusuba, H., In vitro dihydrouridine formation by tRNA dihydrouridine synthase from Thermus thermophilus, an extreme-thermophilic eubacterium. J. Biochem. 158 (6): 513-521 (2015). PMID 26112661. [link to RRC of NBRP] |
user_report | Diederichs, S., Phenotyping the quality of complex medium components by simple online-monitored shake flask experiments. Microb. Cell Fact. 13 (1): 149 (2014). PMID 25376163. [link to RRC of NBRP] |
user_report | Matsumoto, A., Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Thermus thermophilus HB8. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun., 69 (Pt 3): 332-335 PMID 23519816. [link to RRC of NBRP] |
user_report | Tomoike, F., Unique substrate specificity of purine nucleoside phosphorylases from Thermus thermophilus. Extremophiles, 17 (3): 505-514 (2013). PMID 23546840. [link to RRC of NBRP] |
user_report | Hibino, A., Production of uroporphyrinogen III, which is the common precursor of all tetrapyrrole cofactors, from 5-aminolevulinic acid by Escherichia coli expressing thermostable enzymes. Appl. Microbiol. Biotechnol., 97 (16): 7337-7344 (2013). PMID 23604563. [link to RRC of NBRP] |
user_report | Shimada, A., MutS stimulates the endonuclease activity of MutL in an ATP-hydrolysis-dependent manner. FEBS J., 280 (14): 3467-3479 (2013). PMID 23679952. [link to RRC of NBRP] |
user_report | Agari, Y., Crystal structure of hypothetical protein TTHB210, controlled by the σE/anti-σE regulatory system in Thermus thermophilus HB8, reveals a novel homodecamer. Proteins 80 (3): 958-962 (2012). PMID 22231934. [link to RRC of NBRP] |
user_report | Chiba, Y, Discovery and Analysis of Cofactor-dependent Phosphoglycerate Mutase Homologs as Novel Phosphoserine Phosphatases in Hydrogenobacter thermophilus. J. Biol Chem. 287 (15): 11934-11941 (2012). PMID 22337887. [link to RRC of NBRP] |
user_report | Ito, Y., In-cell NMR of intrinsically disordered proteins in prokaryotic cells. Methods Mol. Biol., 895: 19-31 (2012). PMID 22760309. [link to RRC of NBRP] |
user_report | Tomoike, F., A single amino acid limits the substrate specificity of Thermus thermophilus uridine-cytidine kinase to cytidine. Biochemistry 50( 21): 4597-4607 (2011). PMID 21539325. [link to RRC of NBRP] |
user_report | Kondo N., Thermus thermophilus-derived protein tags that aid in preparation of insoluble viral proteins. Anal. Biochem., 385, 278-285 (2009). PMID 19084492. [link to RRC of NBRP] |
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