Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pN1-G-CaMP (#RDB06747)

Genetically-encoded fluorescent calcium sensor cDNA

Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. The expressed protein has proper conformation at 28 degC but not at 37 degC.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AJ550759 (DDBJ accession number)
Depositor|Developer Nakai, Junichi |
 
Sequence RDB06747z.seq
Remarks, protocol and/or map (pdf) RDB06747.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 寄託者に許可を取ること。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。研究成果の発表の時、謝辞に承諾者名を入れる。寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06747 pN1-G-CaMP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pN1-G-CaMP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06747).

Reference section:

Nakai, J., Ohkura, M., Imoto, K., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB06747_A3Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB06747_A3Ega.seq check
>06747_06747_A3Eg_CMV-Forward_A09_03.ab1
    1 AGTTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGCCA
  121 CCATGGTCGA CTCATCACGT CGTAAGTGGA ATAAGACAGG TCACGCAGTC AGAGCTATAG
  181 GTCGGCTGAG CTCACTCGAG AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  241 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  301 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
  361 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  421 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG GGCGGTACCG
  481 GAGGGAGCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
  541 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG
  601 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
  661 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
  721 ACATGAAGCA GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA
  781 CCATCTTCTT CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG
  841 ACACCCTGGT GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC
  901 TGGGGCACAA GCTGGAGTAC AACACGCGTG ACCAACTGAC TGAAGAGCAG ATCGCAGAAT
  961 TCAAAGAAGC TTTCTCCCTA TTTGACAAGG ACGGGGATGG GACAATAACA ACCAAAGGAG
 1021 CTGGGGGACG GTGATGCGGG TCTCTGGGGG CAGAACCCCC ACAGAAGCAG AGCTGCAGGA
1081 CATGATCCAA TGAGTAGAAT GCGACCGTTA TGGCACAATC GAACTTTCCC CTTG
//
Primer: ME-1290Rv
Sequence file: RDB06747_A3Egb.seq check
>06747_06747_A3Eg_ME-1290Rv_B09_06.ab1
    1 AAGGTCAATT TAATTTTTAT GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC
   61 AAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC TAGAGTCGCG GCCGCTCACT
  121 TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG ACCATCCCCA TCGATGTCTG
  181 CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT CTCTCCAAGG TTTGTCATCA
  241 CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC CTTATCAAAC ACACGGAACG
  301 CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT CTTTCATTTT TCTTGCCATC ATTGTCAGGA
  361 ATTCAGGGAA GTCGATTGTG CCATTACCGT CGGCATCTAC TTCATTGATC ATGTCCTGCA
  421 GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCATCAC CGTCCCCAGC TCCTTGGTTG
  481 TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC TTCTTTGAAT TCTGCGATCT
  541 GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT
  601 CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT
  661 TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA
  721 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC
  781 GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA
  841 GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC GTAGGTGGCA TCGCCCTCGC
  901 CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG
  961 GCACCACCCC GGTGAACAGC TCCCTCGCCC TTTGCTCACC ATGCTCCCTC CGGGTACCGC
 1021 CCTTGTACAG CTCGTCCCAT GCCCGAGAGT GATCCCGGCG CGGGTCACGA ACTTCAGCAG
 1081 ACATGTTGAT CGCGGCTCTC GATGGGATCT TGGCTCAAGA CGAACTTGGG CTGCTTCAAG
1141 GTTAAGTGTG TATG
//
Primer: SV40pro_F_V2
Sequence file: RDB06747_A3Egc.seq check
>06747_06747_A3Eg_SV40pro_F_V2_A01_01_ABI08.ab1
    1 TACAGCTTTT TGGAGCTAGG CTTTTGCAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG
  721 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC
  781 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT
  841 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGAAAT GACCGACCAA GCGACGCCCA
  901 ACCTGCCATC ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAAGGTT GGGCTTCGGA
  961 ATCGTTTTCC GGGACGCCGG CTGGGATGAT CCTCCAGCGC GGGGGATCTC ATGCTGGAGT
 1021 TCTTCGCCCA CCCCTACGGG GGAGCTAACT GAAAACACGA AGGAGAACAT TACTGATGAA
 1081 CCCGCGCTAA TGACGGCATT AAAAGACCGA TTAACGCCAC GGATGTTGGG GTCGTTTGAT
1141 TCCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nakai, J., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727. [link to RRC of NBRP]
reference Wang, J.W., Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell, 112, 271-282 (2003). PMID 12553914.