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pN1-G-CaMP1.3 (#RDB06748)

Genetically-encoded fluorescent calcium sensor cDNA

Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. The expressed protein has proper conformation at 28 degC but not at 37 degC.
Comment pN1-G-CaMP1.3 encodes an identical amino acid sequence with that of pN1-G-CaMP, but does not have EcoRI or HindIII in its coding sequence.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 3.9 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Depositor|Developer Nakai, Junichi |
 
Sequence RDB06748z.seq
Remarks, protocol and/or map (pdf) RDB06748.pdf

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks ((Additional form)) Approval Form(FormD)
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提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
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MTAに書く使用条件 寄託者に許可を取ること。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。研究成果の発表の時、謝辞に承諾者名を入れる。寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
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寄託者の書面による提供承諾が必要です。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB06748 pN1-G-CaMP1.3 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pN1-G-CaMP1.3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06748).

Reference section:

Nakai, J., Ohkura, M., Imoto, K., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Nakai, J., A high signal-to-noise Ca(2+) probe composed of a single green fluorescent protein. Nat. Biotechnol., 19, 137-141 (2001). PMID 11175727. [link to RRC of NBRP]
reference Wang, J.W., Two-photon calcium imaging reveals an odor-evoked map of activity in the fly brain. Cell, 112, 271-282 (2003). PMID 12553914.