RDB06749 - pN1-G-CaMP1.6

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pN1-G-CaMP1.6 (#RDB06749)

Genetically-encoded fluorescent calcium sensor cDNA

Clone info.	Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. It showed brighter fluorescence than G-CaMP. The expressed protein has proper conformation at 28 degC but not at 37 degC.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	3,923 bp
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	DQ457005 (DDBJ accession number)
Depositor\|Developer	Nakai, Junichi \|

Sequence	RDB06749z.seq
Remarks, protocol and/or map (pdf)	RDB06749.pdf

Distribution information

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Terms and conditions for distribution This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.

Remarks ((Additional form)) Approval Form(FormD)
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提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Approval form by depositor [Word] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	This material is provided with written consent of Depositor. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the RECIPIENT's name and his/her affiliation.
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 寄託者による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	寄託者に許可を取ること。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。研究成果の発表の時、謝辞に承諾者名を入れる。寄託者から要請がある場合、理研ＢＲＣは利用者情報のうち研究責任者の氏名ならびにその所属機関名を寄託者に通知する。
備考	【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB06749	pN1-G-CaMP1.6	Under QC test. Please contact us.	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pN1-G-CaMP1.6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06749).

Reference section:

Ohkura, M., Matsuzaki, M., Kasai, H., Imoto, K., Nakai, J., Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Ohkura, M., Genetically encoded bright Ca2+ probe applicable for dynamic Ca2+ imaging of dendritic spines. Anal. Chem., 77, 5861-5869 (2005). PMID 16159115. [link to RRC of NBRP]