RDB07461 - pGL4-phSERPINE1(PAI-1)

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pGL4-phSERPINE1(PAI-1) (#RDB07461)

Promoter collection, Human SERPINE1 promoter

Alternative name	U2851-5
Clone info.	PCR amplified human SERPINE1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 101125677 to 101127125(NC_000007.14); relatively -1412 to +37, where +1 corresponds to 1 nt of NM_000602.4.
Comment	PCR cloning, forward primer: GCCACTCCTCATCACTCGCATT; reverse primer: CCCTGCAGCCAAACACAGC. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07461.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07461	pGL4-phSERPINE1(PAI-1)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phSERPINE1(PAI-1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07461).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB13380_A5Dn.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB13380_A5Dna.seq
>07461_13380_A5Dn_pGL4-4174F_A06_03.ab1 1 CTGGTCAGAC TTTCTCTGGC CTACTGGCCG GTACCTGAGC TCGCTAGCCT CGAGGATGCC 61 ACTCCTCATC ACTCGCATTA CCACCAGAGC TCCACTCCCT GTCAGATCAG CGGCGGCATT 121 AGATTCTCAT AGGAGCTCGA ACCCTATTCT AAACTGTTCA TGTGAGGGAT CTAGGTTGCA 181 AGCTCCCTAT GAGAATCTAA TGCCTGATGA TCTGTCACGG TCTCCCATCA CCCCTAGATG 241 GGACCATCTA GTTGCAGGAA AACAAGCTCA GGGCTCCCAC TGATTCTACA CGATGGTGAA 301 TTGTGGAATT ATTTCATTAT ATATATTACA ATGTAATAAT AATAGAAATA AAGCACACAA 361 TAAATGTAAT GTGCTTGAAT CATCCCGAAA CCATCCCACC CTGGTCTGTG AAAAAATTGT 421 CTTCCATGAA ACCAGTCCCT GGTGCCAAAA ACGTTGAGGA CCACTGCTCC ACAGAATCTA 481 TCGGTCACTC TTCCTCCCCT CACCCCCTTG CCCTAAAAGC ACACCCTGCA AACCTGCCAT 541 GAATTGACAC TCTGTTTCTA TCCCTTTTCC CCTTGTGTCT GTGTCTGGAG GAAGAGGATA 601 AAGGACAAGC TGCCCCAAGT CCTAGCGGGC AGCTCGAGGA AGTGAAACTT ACACGTTGGT 661 CTCCTGTTTC CTTACCAAGC TTTTACCATG GTAACCCCTG GTCCCGTTCA GCCACCACCA 721 CCCCACCCAG CACACCTCCA ACCTCAGCCA GACAAGGTTG TTGACACAAG AGAGCCCTCA 781 GGGGCACAGA GAGAGTCTGG ACACGTGGGG GAGTCAGCCG TGTATCATCG GAGGCGGCCG 841 GGCACATGGC AGGGATGAGG GAAAGACCAA GAGTCCTCTG TTGGGCCCAA GTCCTAGACA 901 GACAAAACCT AGACAATCAC GTGGCTGGCT GCATGCCCTG TGGCTGTTGG GCTGGGCCCA 961 GGAGGAGGGA GGGGCGCTCT TTCCTGGAGG TGGTCCAGAG CACCGGGTGG ACAGGCCCTT 1021 GGGGAAAACC TTCACGTTTT GAATGGAAGA TAATCCTTGG ATACTCGACA GTGACCTGGC 1081 TTCGCTAACG AAAAGGCAGG CTAACGGTTG A //
Primer: pGL4-136R Sequence file: RDB13380_A5Dnb.seq
>07461_13380_A5Dn_pGL4-136R_B06_06.ab1 1 GCTATGCTAT GGCATCTTCA TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTTGGCC 61 GCCGAGGCCA GATCTTGATC CCTGCAGCCA AACACAGCTG TGCTCCTCTG TGGGCCACTG 121 CCTCCTTTTA TACCAGATGT GGGCAGGAAA TAGATGAACT CATGTTCCAG CCCCACCCAC 181 TCACTGGCTC TGGGAGTCCG TCTGAACAGC CAGCGGGTCC CCCTCGACAC CTCCCTCTCT 241 GGGACTTGCT GAGGCATGTG TGTGTGTGTG TGTGTGCTGC TCTGTGTGTG TACGTGTGTA 301 AGAGCATTCA GGAACAATTG AGCAAACCCC AATAGCCTTG GCCTGAGAAC CTCCCTTGAC 361 CTTTCTGCCC TCTGCCTGTG TCTGTCTCTC CCGGATGTCC TGCCAGGTTG ACTGTCTGCC 421 ATGCCGGGTG ACCCAAAAAG CCTAGGACCC CTAGTGTTCA GCTTGGAGAA AAAAAAAACA 481 GCTCACGTTG CCTGCTTTTC CTTTGGCGAA CCAGGTCACT GTGGAGTTAT CAAAGATAAC 541 CTCCATCAAA ACGTGGAAGT TTTCCCCCAG GGCTGTCCAC CCGGTGCTCT GGACCACCTC 601 CAGGAAAGAG CGCCCCTCCC TCCTCCTGGG CCCAGCCCAA CAGCCACAGG GCATGCAGCC 661 AGCCACGTGA TTGTCTAGGT TTTGTCTGTC TAGGACTTGG GCCCAACAGA GGACTCTTGG 721 TCTTTCCCTC ATCCCTGCCA TGTGCCCGGC CGCCTCCGAT GATACACGGC TGACTCCCCC 781 ACGTGTCCAG ACTCTCTCTG TGCCCCTGAG GGCTCTCTTG TGTCAACAAC CTTGTCTGGC 841 TGAGGTTGGA GGTGTGCTGG TGGGGTTTGT GTGGGGGGGG TCTTGAAACG GGAAACCCAA 901 GGGGGTATAC CCATGGTAAA AAGCCTTTGG GTTAAGGGAA AACAGGGAGA CCCAAACGTG 961 TAAAGTTTTC ACTTTCCCTC GAGCTGCCCC GCTAGGACTT TGGGGGCAGC TTTGTTCCCT 1021 TTTATCCCTC TTTCCCTCCA GACACAGACA CAAAGGGGAA AAAGGCATAG AAACAGAGTG 1081 GTCCAATTCC ATGGCCAAGT TTGCCATGGT GTGCTTTTAC GGCCAGCGTG AAGGGAGAGA 1141 GGACCGATAG GTAATTCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Resmini, G., HMGA1 regulates the Plasminogen activation system in the secretome of breast cancer cells. Sci. Rep. 7 (1): 11768 (2017). PMID 28924209. [link to RRC of NBRP]