RDB07487 - pGL4-phPLAU

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pGL4-phPLAU (#RDB07487)

Promoter collection, Human PLAU promoter

Alternative name	U2833-5
Clone info.	PCR amplified human PLAU promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 73909783 to 73911185(NC_000010.11); relatively -1318 to +85, where +1 corresponds to 1 nt of NM_002658.4.
Comment	PCR cloning, forward primer: GGGTTCAAAATGACCCCAAG; reverse primer: TGCGGGGACAGGTGGACCCT. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human plasminogen activator, urokinase genomic DNA
Depositor\|Developer	DNA Bank, \|

Remarks, protocol and/or map (pdf)	RDB07487.pdf

Distribution information

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The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07487	pGL4-phPLAU	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGL4-phPLAU was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07487).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07487_A6Gep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Sequence file: RDB07487_A6Gea.seq
>07487_07487_A6Ge_pGL4-4174F_F01_16.ab1 1 AAGGGTCAGA CTTTCTCTGG CCTAACTGGC CGGTACCTGA GCTCGCTAGC CTCGAGGATG 61 GGTTCAAAAT GACCCCAAGC CCTGGTTCCT GACACTAGGG GAAAGAGATG GGGGTGACAG 121 AATCACAGAA TCCCTGCTAT GTTCCTCCAA GTGTGCCCAG AGATGCGTGT GTGTGTGTGT 181 GTATACACAA ATGTCTGCTT ATCCTCAGGC AGGAAGGGTG GATGCAGTCA TTTACACATG 241 GTCTGTTTTT CTGGAGGACA ATTTTATTTG ATAAACAATT GTTTCTATCT GAATAGAATA 301 AACAAGGCTC TATGATGAAG TAAAACACTA AATACACATG CATTAAAAAA TGCATAATTA 361 TCTTTTTGGA ATGGGCTATA CAGAGATGTG CTTTTTAAAA TGTTAAGAGT GTAAAAGGAC 421 AAACAGTGAA AAATAAATCT TCCTCTTATT TTGTCCTCCA GTCTCCCAAT TCCTCTACTC 481 AGAGGTGAGA ACAGAACTTC CACACCCTCC AGAACCTCCA CAGTTAGAAC TGTCTACATG 541 TTTCCATTGT CTTTACTTTT ATTCTTGCCT GCACAAATAA ATGAATTGCT CATTATGGAA 601 ACTTCCCAAA AGACCCGTTA ACACTTCAAT AGGAAGCACC AACAGTTTAT GCCCTAGGAC 661 TTTGTTCCCA CAATCCTGTA ACATCATATC ACGACACCTA ACCCAATCCT TATCAAGCCC 721 TGTCAAAAAC GGACTTTAAA CCAAGCTGCA AATTTTCAGT AATCTGGCCT TGCCTTTCCC 781 CCTCTGATAG CACCATCAAA CAAACCCCCT TACTGCCGAA AGCAATAAGC CCGGCTTTGT 841 TCCATCCACT GGTTGTGTTG GTGATATCTG GGGACTGCCA CTGAACAGAC GCACAGAGGG 901 AGCCCCTACA GGCACGGGTT TTTCTGTCTG TGCTTCTGGG AGAGTATGTC TCGTACATTT 961 TGTCGCGTTG ATGAAAGACT TCACAGCTCC ATCAGCTGCG GGCAAAGGGG TCTGGAGCAG 1021 TCTTTCGCAG TTGGGGGCCC AGCGGGGGAA GAAGCTGCCA GAGCACCTGA ATTAGAGGAA 1081 CCCCCAGGGC AAGGGT //
Primer: pGL4-136R Sequence file: RDB07487_A6Geb.seq
>07487_07487_A6Ge_pGL4-136R_G01_19.ab1 1 GCTTAGTTTT GGCATCTTCA TGGTGGCTTT ACCAACAGTA CCGGATTGCC AAGCTTGGCC 61 GCCGAGGCCA GATCTTGATT GCGGGGACAG GTGGACCCTG GCCCGGGCTC CGGGGCTGCG 121 GTCTCCGCAC TGTGCTGCGA CCCGCGGCGC CTGCTCTATA TCAGGGCCCG CCCCGGCGCC 181 GCCCCTCCCT CTCCCGCCCG GCTCCCTCCC CTGTCTTGCA GCGCTCAGCG ACCCGGACCC 241 CGCGTGCTTC CGCAACGCTC ACAAAGATTT GGGGGAAGCG CGATCTCCAG CGGAGGGGAC 301 CCAACAGCGT CTGGACTGAG GAATCGAGAG GCTTGTAAAT TCTCCGTGCT TCCTCCCATG 361 CACCTGGCCG GGGGCCTGCC CCAGTGCAAG GAGTCCCCGA ATTGCAGAGA GGAGAGAAGG 421 CGCACAGGAG ACTCTCTACC TCGCCCAGCT CTGAAGCCTC CTGGGGTCCT CTAATCAGTT 481 CTTCTGCAAC TTCTCCCCGC TGGGCCCCAA CTTGCCTAAG ACTGCCTCAG ACCCCCTTGC 541 CCGCAGCTGA TGGAGCTGTG AAGTCTTCAT CAACGCGACA AATGTACGAG ACATACTCTC 601 CCAGAAGCAC AGACAGAAAA ACCCCTGCCT GTAGGGGCTC CCTCTGTGCG TCTGTTCAGT 661 GGCAGTCCCC AGATATCACC AACACAACCA GTGGATGGAA CAAAGCCGGG CTTATTGCTT 721 TCGGCAGTAA GGGGGTTTGT TTGATGGTGC TATCAGAGGG GGAAAGGCAA GGCCAGATTA 781 CTGAAAATTT GCAGCTTGGT TTAAAGTCCG TTTTTGACAG GGCTTGATAA GGATTGGGTT 841 AGGTGTCGTG ATATGATGTT ACAGGATTGT GGGAACAAAG TCCTAGGGCA TAAACTGTTG 901 GTGCTTCCTA TTGAAGTGTT AACGGGTCTT TTGGGAAAGT TTCCATAATG AGCAAATTCA 961 TTTATTTGTG CAGGCAAGAA TAAAAGTAAA GACATGGGAA ACATGGTAGA ACAGCTCTTA 1021 CTGTTGGAGG ATCCTGAAGG GATGTGAAAG GTCTGATCTC ACATCTGAGT AGAGAACTTG 1081 GGGGAGAACT GG //
Primer: pAxCALNL_F1 Sequence file: RDB07487_A6Gec.seq
>07487_07487_A6Ge_pAxCALNL_F1_H01_22.ab1 1 TATTATCTTA TCATGTCTGC TCGAGCGGCC GGCCGCCCCG ACTCTAGAAT TATTACACGG 61 CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG GCGTCCAACT 121 TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC AGCTTCTTGG 181 CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG GTTTTACCGT 241 GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC AGGCCGGCGA 301 CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG GCTGGGGCTA 361 CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG AAGTGCTCGT 421 CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCAGCCAGCC GTCCTTGTCG ATGAGAGCGT 481 TTGTAGCCTC GGGGTTGTTA ACGTAGCCGC TCATGATCAT GGGGCCACGG ACGCACAGCT 541 CGCCGCGCTG GTTCACACCC AGTGTCTTAC CGGTGTCCAA GTCCACCACC TTAGCCTCGA 601 AGAAGGGCAC CACCTTGCCT ACTGCGCCAG GCTTGTCGTC CCCTTCGGGG GTGATCAGAA 661 TGGCGCTGGT TGTTTCTGTC AGGCCGTAGC CCTGGCGGAT GCCTGGTAGG TGGAAGCGTT 721 TGGCCACGGC CTCACCTACC TCCTTGCTGA GCGGCGCCCC GCCGCTGGCG ATCTCGTGCA 781 AGTTGCTTAG GTCGTACTTG TCGATGAGAG TGCTCTTAGC GAAGAAGCTA AATAGTGTGG 841 GCACCAGCAG GGCAGATTGA ATCTTATAGT CTTGCAAGCT GCGCAAGAAT AGCTCCTCCT 901 CGAAGCGGTA CATGAGCACG ACCCGAAAGC CGCAGATCAA GTAGCCCAGC GTGGGTGAAA 961 CATGCCGAAG CCGTGGTGAA ATGGGCACCA CGCTGAGGAT AGCGGTGTCG GGGATGATCT 1021 GGTTGCCGGA GATGGGGTCG CGGGGCATGA CTGCATCCGG ACAACAAAGG CCGGATGGCC 1081 GGATTGAGCG GATAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report	Resmini, G., HMGA1 regulates the Plasminogen activation system in the secretome of breast cancer cells. Sci. Rep. 7 (1): 11768 (2017). PMID 28924209. [link to RRC of NBRP]