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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMT-PURO (#RDB08532)

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.

Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Growth conditions 37C, LB + Amp
Depositor|Developer Iwaki, Takayuki |
 
Sequence (full) RDB08532zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology 57(1), 45-49, 2008).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cytotechnology 57(1), 45-49, 2008)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08532 pMT-PURO DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMT-PURO was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08532).

Reference section:

Iwaki, T., Castellino, F.J., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB08532_A2Gap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: MT pro, BiP signal sequence
Sequence file: RDB08532_A2Gaa.seq check
>08532_08532_B1CtF_HB-NOS_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNT TGGGTNNNGC CNGGGTTTTC CCNGTCACGA CGTTGTAAAA
   61 CGACGGCCAG TGAATTAATT CGTTGCAGGA CAGGATGTGG TGCCCGATGT GACTAGCTCT
  121 TTGCTGCAGG CCGTCCTATC CTCTGGTTCC GATAAGAGAC CCAGAACTCC GGCCCCCCAC
  181 CGCCCACCGC CACCCCCATA CATATGTGGT ACGCAAGTAA GAGTGCCTGC GCATGCCCCA
  241 TGTGCCCCAC CAAGAGCTTT GCATCCCATA CAAGTCCCCA AAGTGGAGAA CCGAACCAAT
  301 TCTTCGCGGG CAGAACAAAA GCTTCTGCAC ACGTCTCCAC TCGAATTTGG AGCCGGCCGG
  361 CGTGTGCAAA AGAGGTGAAT CGAACGAAAG ACCCGTGTGT AAAGCCGCGT TTCCAAAATG
  421 TATAAAACCG AGAGCATCTG GCCAATGTGC ATCAGTTGTG GTCAGCAGCA AAATCAAGTG
  481 AATCATCTCA GTGCAACTAA AGGGGGGATC CGATCTCAAT ATGAAGTTAT GCATATTACT
  541 GGCCGTCGTG GCCTTTGTTG GCCTCTCGCT CGGGAGANCT TCGCGAACTA GTCCGGANAT
  601 CCACGTGTGT ACACCTANGC TTAAGTCTAG AGGGCCNTTC NAANGNAAGC CTATCCCTAA
  661 CCCTCTCCTC NGTCTCGANT CNACNCNNAN CNGTCATCAT CNNNATCNCN ANNNNNTTNN
721 ANNCNNNNNN NNNN
//
Primer: reverse (Pr0099)
Region: SV40 pA, 6xHis, V5 tag, BiP signal sequence, MT pro
Sequence file: RDB08532_A2Gab.seq check
>08532_08532_B1CtF_reverse_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNCNNNNNNN NNTAATCNGC CNTACCACAT TTGTAGAGGT TTTACTTGCT
   61 TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT
  121 GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
  181 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
  241 TCTTATCATG TCTGGATCCT CGATCGAGGC TGATCAGCGA GCTCAGGCCT TAGAAGGCAC
  301 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTACGCGT
  361 AGAATCGAGA CCGAGGAGAG GGTTAGGGAT AGGCTTACCT TCGAAGGGCC CTCTAGACTT
  421 AAGCCTAGGT GTACACACGT GGATATCCGG ACTAGTTCGC GAAGATCTCC CGAGCGAGAG
  481 GCCAACAAAG GCCACGACGG CCAGTAATAT GCATAACTTC ATATTGAGAT CGGATCCCCC
  541 CTTTAGTTGC ACTGAGATGA TTCACTTGAT TTTGCTGCTG ACCACAACTG ATGCACATTG
  601 GCCAGATGCT CTCGGTTTTA TACATTTTGG AAACNCGGCT TTACACACGG GTCTTNCGTT
661 CNANTNNCCT CNTTNNNANN CNNNNGNNNN GCTNNNAANT NNNNNNNNNN
//
Primer: COPIApro-F (Pr0259)
Region: copia pro, PuroR
Sequence file: RDB08532_A2Gac.seq check
>08532_08532_B1CtF_COPIApro-F_G02_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCTT TCCTTCTTGT ACGTTTTTTG CTGTAAGTAG GTCGTGGTGC
   61 TGGTGTTGCA GTTGAAATAA CTTAAAATAT AAATCATAAA ACTCAAACAT AAACTTGACT
  121 ATTTATTTAT TTATTAAGAA AGGAAATATA AATTATAAAT TACAACAGAT CCCCCGGGCT
  181 GCAGGAATTC GATAGCTTGC ATGCCTGCAG GTCGGCCGCC ACGACCGGTG CCGCCACCAT
  241 CCCCTGACCC ACGCCCCTGA CCCCTCACAA GGAGACGACC TTCCATGACC GAGTACAAGC
  301 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG
  361 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG
  421 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
  481 TCNNGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC NGANAGCGTC GAAGCGGGGG
  541 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGANTTGAG CGGNTCCNGG CTGGNCGCGC
  601 ANCAACAGAN GGNANGGNCT CCTGGCGCCN CANCNGCCNN ANGNANCCCN NGNNGNNNCN
661 NGNNNNNNGN NNNNNTCNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, SV40 pA
Sequence file: RDB08532_A2Gad.seq check
>08532_08532_B1CtF_Amp_R_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNTNANCAT TTATNNGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
   61 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
  121 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG
  181 CCCTTTCGTC TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG
  241 GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG
  301 TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA
  361 CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC
  421 ATCAGGCGCC GGTACCCGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA
  481 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT
  541 TTGTAACCAT TATAAGCTGC AATAAACAAG TTCAAGCTAG CTGGCCTCGA NGTCGACGGT
  601 ATCGATAAGC TTGATCTAGA GTCNGTGGGC CTCGGGGGCG GGNGCGGGGG NCGGCGGGGC
  661 CGCCNNNGGN NGNTTNNNNC NNNNCCNTGG GGNCNNGNGC TNNNTTNNGN NNNGGNNNNN
721 NNGNNNNNNG GGGNNNGGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. [link to RRC of NBRP]
reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017). [link to Journal]
user_report Védrine, M., Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14. PLoS One 13 (8): e0202664 (2018). PMID 30142177. [link to RRC of NBRP]
user_report Niwa, Y., Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol. Biol. Cell 27 (5): 744-756 (2016). PMID 26764097. [link to RRC of NBRP]