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pMT-PURO (#RDB08532)

Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Iwaki, T., Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Metametallothionein promoter expression vector with puromycin resistance gene for Schneider 2 (S2) Drosophila cells. Puromycin serectable.
Vector backbone pMT-PURO (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Growth conditions 37C, LB + Amp
Depositor|Developer Iwaki, Takayuki |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Cytotechnology 57(1), 45-49, 2008).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Cytotechnology 57(1), 45-49, 2008)。

Catalog # Resource name Shipping form Fee
RDB08532 pMT-PURO DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMT-PURO was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08532).

Reference section:

Iwaki, T., Castellino, F.J., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection in Drosophila Schneider S2 cells expressing heterologous proteins. Cytotechnology 57 (1), 45-49, (2008). PMID 19003171. [PubMed] [Article] [RRC of NBRP]
reference Zitzmann, J., Process Optimization for Recombinant Protein Expression in Insect Cells. New Insights into Cell Culture Technology, Dr. Sivakumar Joghi Thatha Gowder (Ed.), InTech (2017). [Article]
user_report Vedrine, M., Sensing of Escherichia coli and LPS by mammary epithelial cells is modulated by O-antigen chain and CD14. PLoS One 13 (8): e0202664 (2018). PMID 30142177. [PubMed] [Article] [RRC of NBRP]
user_report Niwa, Y., Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells. Mol. Biol. Cell 27 (5): 744-756 (2016). PMID 26764097. [PubMed] [Article] [RRC of NBRP]