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pMAK42 (#RDB09003)

Expression vector under the control of tet inducible promoter. Puromycin serectable

Clone info. Expression vector under the control of tet inducible promoter. Puromycin serectable.
Vector backbone pMAK42 (Plasmid)
Size of vector backbone 9.2 kb
Selectable markers Amp^r
Depositor|Developer Iwaki, Takayuki |
 
Sequence (full) RDB09003zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)).
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。
備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09003 pMAK42 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMAK42 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09003).

Reference section:

Iwaki, T., Umemura, K., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09003_A3Fb.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_22mer
Sequence file: RDB09003_A3Fba.seq check
>09003_09003_A3Fb_T7_22mer_E06_15.ab1
    1 TGCTGAGGCA GTGAAAATGC TTTATTTGTG AATTTGTGAT GCTATTGCTT TATTTGTAAC
   61 CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT
  121 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
  181 TGATTATGAT CCTCTAGAGA TATCGCTAGC ACGCGTTTCG AATCCGGAGC ATGCAGATCT
  241 GGCGCGCCAC CGGTCTTAAG ATGCATGGTG GCGGCGATCC CCGGGTACCG AGCTCGAATT
  301 CTCCAGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC CCACCGTACA
  361 CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA TACGTTCTCT
  421 ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC
  481 ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC ACTGATAGGG
  541 AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT
  601 CACTGATAGG GAGTAAACTC GAGGAGCCCT AGGTTGGCCC ATTGCATACG TTGTATCCAT
  661 ATCATAATAT GTACATTTAT ATTGGCTCAT GTCCAACATT ACCGCCATGT TGACATTGAT
  721 TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG
  781 AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC
  841 GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT
  901 GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC
  961 ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA TGGGCCGCCT GGCATTATGC
 1021 CAGTACATGA CCTTATGGAA CTTTCTACTG GCAGTACATC TACGTATAGG TCATCGCTAA
 1081 TACAATGTGA TGCGAATGGC AGTACATCCA TGGGCGGTGC AATAGGCCGG GTTTTGACGT
1141 CA
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB09003_A3Fbb.seq check
>09003_09003_A3Fb_SV40pA3'Element Reverse_F06_18.ab1
    1 GTAGGCAGGT GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA
  301 GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT
  361 GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG
  421 CTATGACCAT GATTACGCCA AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA
  481 GCTCACCCGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC ACAACTAGAA
  541 TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA
  601 TTATAAGCTG CAATAAACAA GTTCAAGCTG CGGCCGCCGT ATCGAGGTCG ACGGTATCGA
  661 TAAGCTTGAT CTAGAGTCGG TGGGCCTCCT ACAGGAACAG GTGGTGGCGG CCCTCGGTGC
  721 GCTCGTACTG CTCCACGATG GTGTAGTCCT CGTTGTGGGA GGTGATGTCC AGCTTGGGCG
  781 TCCACGTAGT AGTAGCCGGG CAGCTGCACG GGCTTCTTGG CCATGTAGAT GGACTTGAAC
  841 TCCACCAGGT AGTGGCCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGGTC TCGCCCTTCA
  901 GCACGCCGGT CGCGGGGGGG TACAGGCGCT CGGGTGGAGG CCTCCTCAGC CCATGGATCT
  961 TTCTTCTGCA TCAACGGGGG TCGTCGAGGG GAAGTTCACG TCGATGAACC TTCACATTGT
1021 AGATGAGGCC AGTCGTTCCT GCAATGAAGA AGTCCTGGGT CACGATCGCT ACGTCGGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP]