RDB12875 - CSIV-TRE-GFP-CMV-KT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-TRE-GFP-CMV-KT (#RDB12875)

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.

Clone info.	Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by CMV promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-TRE-RfA-CMV-KT ()
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12875zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB12875.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12875	CSIV-TRE-GFP-CMV-KT	Please contact us.	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-TRE-GFP-CMV-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12875).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB17885_B0Atp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N (Pr0027) Region: attB1,tight TRE Pro,cPPT_CTS Sequence file: RDB17885_B0Ata.seq
>12875_17885_B0At_1_EGFP-N_A11_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNN NNNNNGGTGG AGCCTGCTTT TTTGTACAAA 61 CTTGTGATGC TAGCGGCGAT CTGACGGTTC ACTAAACGAG CTCTGCTTAT ATAGGCCTCC 121 CACCGTACAC GCCTACCTCG ACATACGTTC TCTATCACTG ATAGGGAGTA AACTCGACAT 181 ACGTTCTCTA TCACTGATAG GGATAAACTC GACATACGTT CTCTATCACT GATAGGGAGT 241 AAACTCGACA TACGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG TTCTCTATCA 301 CTGATAGGGA GTAAACTCGA CATCGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC 361 GTTCTCTATC ACTGATAGGG AGTAAACTCG ATTGAAGACG AAAGGGCCGA ATTCAAAGTG 421 AATTTCTGCT GTCCCTGTAA TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA 481 ATTCTTTAGT TTGTATGTCT GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC 541 CCCCAATCCC CCCTTTTCTT TTAAAATTGT GGATGAATAC TGCCATTTGT AGATCCGTTC 601 ACTAATCGAA TGGATCTGTC TCTGTCTCTC TCTCCACCTT CTTCTTCTAT TCCTTCGGGC 661 CTGTCGGGTC CCCTCGGGGT TGGGAGGTGG GTCTGAAACG ATAATGGTGA ATATCCCTGC 721 CTAACTCTAT TCACTATAGA AAGTACAGCA AAAACTATTC TTAAACCTAC CAAGCCTCCT 781 ACTATCATTA TGAATAATTT TATATACNAC AGCCAATTTG TTATGTTAAA CCNATTCCNC 841 AAACTTGCCC ATTTATCTAA TTCCAATAAT TCTTGTTCAT TCTTTTCTTG NTGNNTTTGC 901 GATTCNTCAA TTAAGGAGTG NATTAANCTT GTGNNATTGT TAATTTCTCN GNNCNNCTNN 961 NNNCANGNCG NGNGATNNCN AATCTGNTNN ANANATTTAT TANNCCNNCN ANNATNNNNN 1021 NNNNN //
Primer: FP-C_F1 (Pr0772) Region: EGFP 3',CMV en.2 Sequence file: RDB17885_B0Atb.seq
>12875_17885_B0JkF_FP-C_F1_H02_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NCGCGNTCNC ATGGTCCTGC TNNNNNNCGT GACCGCCGCC 61 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC TCAGATCCTA CTAGTAGGAT 121 CTCGAGATAT CTAGACCCAG CTTTCTTGTA CAAAGTGGTG ATGCTACCGG TCTCGAGGAG 181 CTTGGCCCAT TGCATACGTT GTATCCATAT CATAATATGT ACATTTATAT TGGCTCATGT 241 CCAACATTAC CGCCATGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG 301 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC 361 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC 421 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT 481 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT 541 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT 601 TGGCAGTACA TCTACGTATT AGTCNTCGCT ATTACCATGG TGANGCGGNT TTGGNAGTAC 661 ATCNATGGGG CGTGGANAGC GGTTTGACTC NCGGGGNNTT CCNNNNCTCC NCCCCNTTGN 721 CNTCNNTNGG ANTTNGNTTN NGNNNNNAAN NNNNNNGGNN NNTTNNNNAA NNN //
Primer: PRE_rev (Pr0250) Region: PRE,rt TA Advanced Sequence file: RDB17885_B0Atc.seq
>12875_17885_B0At_1_PRE_rev_G11_20_ABI24.ab1 1 NNNNNNNNNN NNNNCGNNNA NNGAGCANCA TAGTTAAGAA TACCAGTCAA TCTTTCNCAA 61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG 121 ATCCTTACTT AGTTACCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG 181 CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC 241 GTCAAGGGCG TCGGCCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT 301 GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT 361 TGCTTGTCTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC 421 TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG 481 AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC 541 CAGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCGCG 601 ATGTGAGAGG AGAGCACAGC GGAATGACTT GGCGTTGTTC CGCAGAAAGT CTTGCCATGA 661 CTCGCCTTCC AGGGGGCAGA AGTGGGTATG ATGCCTGTCC AGCATCTCGA TTGGCAGGGC 721 ATCGAGCAGG GCCCGCTTGT TCTTCACGTG CCAGTACAGG GTAGGCTGCT CAACTCCCAG 781 CTTTTGAGCG AGTTTCCTTG TCGTCAGGCC TTCGATACCG ACTCCATTGA GTAATTCCAG 841 ANCGCCGTTT ATGANTTTGC TCTTGTCCNG TCTAGACATA NGGCCGGGAT TCTCCTCCNC 901 GTCACCGCAN GTTAGAAGAN NTCCNCTGNC CNCGCANTGG GCCACGGNGT CCNNCACCAN 961 CNCGGNGANG TTGNCNNCGG NCTTNNNNNN NANNNTGNNG NNNNANGNNN TNNCNNTGGG 1021 GNNTCNTNNN NNNNNNN //
Primer: tTA-Ad R (Pr0542) Region: T2A,hKO Sequence file: RDB17885_B0Atd.seq
>12875_17885_B0At_1_tTA-AdR_F11_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNTNNN GANTTTGCTC TTGTCNNGTC TAGACNTAGG GCCGGGNTTC 61 TCCTCCACGT CACCGCATGT TAGAAGACTT CCTCTGCCCT CGCAGTGGGC CACGGCGTCC 121 TCCACCAGCT CGGTGATGTT GCCCTCGGTC TTCCTCACCA GCCTGTGGCC GATGAAGTGG 181 CTCTGGGGCA TCTTCAGGAT CTTCTTGGCG GCCTTGTAGG TGGTCTTGAA CTGGCACTTG 241 TGGTTGCCGC CGCCGGCCAG CTTCAGGTAC ATGGTCACGT CGCCCTTCAG CACGCCGTCG 301 CAGGTGGTGA TCTTCTCGGT GCTGGGCTCC CAGTCGCTGC TCTGGTTCTG CATCACGGGG 361 CCGTCGGCGG GGAAGTTCAC GCCCACGAAC TTGCTCTTGT GCTCGAAGCA GTTGCCCCTC 421 AGGCTGATGT GGGCGCTCAC GGCGGCGAAG CCGCCGTCCT CGAACTGGAG GCTCCTCTCC 481 CAGCTCAGGC CCTCGGGGAA GGCCTGCTTG AAGTAGTCGG GGATCTCCTC GGGGTACTTG 541 GTGAAGGGCC TGTGGCCGTA GCAGAAGGTG TGGCTCACCA GGTCGAAGCT GAAGGGCATG 601 GGGCCGCCCT TGGCCATTGT CACCCTCAGG GTCATCTCCT GGTGGCCCTC GTAGGGCTTG 661 CCGGTGCCCT CGCCCTCCAC GGTGAACTCG TGGCCGTTCA CGCTGCCGTC CATGAAGTAC 721 TTCATCTTCA TCTCGGGCTT GATCACGCTC ACCATGGTGG ANCGGTGAAT TCGGGGCCGC 781 GGANGCTGGA TCGGTCCCGG TGTCTTCTAT GGANGTCAAA ACAGCGTGGA TGGCGTCTCC 841 AGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA GACCTCCNAC CGTACACGCC 901 TACNGCCCAT TTGCGTCNAT GGGGGCGGAN TTGTTACNAN NTTTNNGAAA GTCCCGTTGA 961 TTTTGGTGNC NAAACAAACT CCNNTTGNNG TNNNNGGGGG NNNNNNNTNN NNANTCCNNN 1021 NNANNCNAAN CNGNNNTCNN NNNNNNNN //
Primer: HIV-B_R (Pr0348) Region: HIV-1 psi,5' LTR,CMV en.1,CMV pro1 Sequence file: RDB17885_B0Ate.seq
>12875_17885_B0At_1_HIV-B_R_C11_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNANNTTTT GGNGTACTCN CCAGTCGCCG CCCCTCGCCT 61 CTTGCCGTGC GCGCTTCAGC AAGCCGAGTC CTGCGTCGAG AGAGCTCCTC TGGTTTCCCT 121 TTCGCTTTCA AGTCCCTGTT CGGGCGCCAC TGCTAGAGAT TTTCCACACT GACTAAAAGG 181 GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC ACAACAGACG GGCACACACT ACTTGAAGCA 241 CTCAAGGCAA GCTTTATTGA GGCTTAAGCA GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC 301 AGGCTCAGAT CTGGTCTAAC CAGAGAGACC CAGTACAGGC AAAACGCGCT GCTTATATAG 361 ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT GTTACGACAT 421 TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC AATGGGGTGG 481 AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA CTGCCAAAAC 541 CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT AGGAAAGTCC 601 CATAAGGTCA TGTACTGGGC ATAATGCCAG GCGGGCCATT TACCGTCATT GACGTCAATA 661 GGGGGCGTAC TTGGCATATG ATACACTTGA TGTACTGCCA AGTGGGCAGT TTACCGTAAA 721 TACTCCACCC ATTGACGTCA ATGGAAAGTC CCTATTGGCG TTACTATGGG AACATACGTC 781 ATTATTGACG TCAATGGGCG GGGGTCGTTG GGCGGTCAGC CAGGCGGGCC ATTTACCGTA 841 AGTTATGTAA CGCGGAACTC CATATATGGG CTATGAACTA ATGANCCCGN AATTGATTAC 901 TATTAATAAC TAGTCAATAA TCNATGTCNA CGCGTATATC TGGNCCNNAN ATCGCGAANC 961 AGCGCAAAAC GCCNAACCNT AANCAGANTC TTCNTGNNNN TGNCNGNCNN NNNTNNNNNN 1021 NNTNNNANCN NNAATTTTGN NCNCNNNNNN NNNN //
Primer: attB2-R1 (Pr0411) Region: EGFP mid,Tight TRE pro Sequence file: RDB17885_B0Atf.seq
>12875_17885_B0At_1_attB2-R1_D11_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNCTGA NTCCGGANTT GTACNGCTCG TCCNTGCCGA 61 GAGTGATCCC GGCGGCGGTC ACGAACTCCA GCAGGACCAT GTGATCGCGC TTCTCGTTGG 121 GGTCTTTGCT CAGGGCGGAC TGGGTGCTCA GGTAGTGGTT GTCGGGCAGC AGCACGGGGC 181 CGTCGCCGAT GGGGGTGTTC TGCTGGTAGT GGTCGGCGAG CTGCACGCTG CCGTCCTCGA 241 TGTTGTGGCG GATCTTGAAG TTCACCTTGA TGCCGTTCTT CTGCTTGTCG GCCATGATAT 301 AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG CCGTCCTCCT 361 TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG AACTTCACCT 421 CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC TGGACGTAGC 481 CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG TAGCGGCTGA 541 AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG GGCAGCTTGC 601 CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC TCGCCCTCGC 661 CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG ATGGGCACCA 721 CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGA GCCTGCTTTT TTGTACAAAC 781 TTGTGATGCT AGCGGCGATC TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC 841 ACCGTACACG CCTACCTCGA CATACGTTCT CTATCACTGA TANGGAGTAA ACTCGACATA 901 CGTTCTCTAT CACTGATAGG GANAAACTCG ACNTACGTTC TCTATCNNNG ATANGGAGTA 961 AANTCGACNT NCGTTCTCTA TCANNNATAG GGNNTAAACT NNNNNTACNT TCNNNANNAC 1021 NNNTANGGNN TAANNNNNNN NTNNNNNNNN ATCNNTNNNN NGNNNNNANN NNNNNNNN //
Primer: BGH_rev2 (Pr0606) Region: bGHpA,3' LTR,lox P,PRE Sequence file: RDB17885_B0Atg.seq
>12875_17885_B0At_1_BGH_rev2_H11_23_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGGGGNNGGG CNNACNACAG ATGGCTGGCA ACTAGAAGGC 61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAA TAACTTCGTA 301 TAATGTATGC TATACGAAGT TATGATATCT TGTCTTCGTT GGGAGTGAAT TAGCCCTTCC 361 AGTCCCCCCT TTTCTTTTAA AAAGTGGCTA AGATCTACAG CTGCCTTGTA AGTCATTGGT 421 CTTAAAGGTA CCTGAGGTGT GACTGGAAAA CCCACCTCCT CCTCCTCTTG TGCTTCTAGC 481 CAGGCACAAT CAGCATTGGT AGCTGCTGTA TTGCTACTTG TGATTGCTCC ATGTTTTTCT 541 AGGTCTCGAC GGTATCGATG CGGGGAGGCG GCCCAAAGGG AGATCCGACT CGTCTGAGGG 601 CGAAGGCGAA GACGCGGAAG AGGCCGCAGA GCCGGCAGCA GGCCGCGGGA AGGAAGGTCC 661 GCTGGATTGA GGGCCGAAGG GACGTAGCAG AAGGACGTCC CGCGCAGAAT CCAGGTGGCA 721 ACACAGGCGA GCAGCCATGG AAAGGACGTC AGCTTCCCCG ACAACACCAC GGAATTGTCA 781 GTGCCCAACA GCCGAGCCCC TGTCCAGCAG CGGGCAAGGC AGGCGGCGAT GANTTCCGCC 841 GTGGCAATAG GGANGGGGAA AGCGAAAGTC CNGGAAAGGA NCTGANAGGN GGTGGNAATG 901 NCCCAACCNG NGGGGGTTGC GTCAGCAAAC ACAGNGCACA NCACGCNNCG TTGNCNGANN 961 ACGGGCCNCA NNTCCTCATA AAGANNNAGC AANCANNNNT TNTANNNNNA NNANAAAATG 1021 AAAGCNNTAN NGGNNNNNNT ANCNNNNNNN NANNNNNTTN ANNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo Sequence file: RDB17885_B0Ath.seq
>12875_17885_B0At_1_SV40pro_ori_F_A12_03_ABI24.ab1 1 NNNNCNNNNN NNNNGNNNCC TCTGCCTCTG ANCTATTCNN GAAGTAGTGA GGAGGCTTTT 61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA 181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA 241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA 301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA 361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA 421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC 481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG 541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC 601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 661 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 721 TAATGNTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 781 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 841 GANCTCTAGC TAGAGCTTGG CGTANTCATG GTCNTAGCTG TTTCCTGTGT GAAATTGTTA 901 TCCGCTCACA ATTCCNCACA ACATACGANC NNGAAGCATA AAGTGNAAAG NCTGGGGNGN 961 CTAATGANTG AGCTANNNCN CATTNNTTGC GTNGCGNNNA CNGNCNNCTT TNNNNNNNGN 1021 NNANNNNNNN NNGNNNGCNN NNNTNNNNNN NNNN //