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pCAG-Fucci2a (#RDB13080)

Fucci2a cell cycle reporter.

Clone info. Fucci2a cell cycle reporter composed of Fucci2, mVenus-hGem(1/110) and mCherry-hCdt1(30/120).
Vector backbone pCAGiP (Plasmid)
Selectable markers Ampicillin
Gene/insert name Human Cdt1 cDNA Human Gem cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA
Depositor|Developer Mort, Richard |
 
Sequence (full) RDB13080hts01.seq checkAssembled from experimentally sequenced data.
Publication Mort, R.L., Cell Cycle 13 (17): 2681-2696 (2014). [link to RRC of NBRP]
Test sheet RDB13080_A8Lkp1-2.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
(3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
(4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, 2014) and an acknowledgment to the DEPOSITOR are requested.
(3) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
(4) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
付加的使用条件:
1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki.
備考 提供条件は英文のまま記載してください。
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13080 pCAG-Fucci2a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge


How to cite this biological resource

Materials & Methods section:

The pCAG-Fucci2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13080).

Reference section:

Mort, R.L., Ford, M.J., Sakaue-Sawano, A., Lindstrom, N.O., Casadio, A., Douglas, A.T., Keighren, M.A., Hohenstein, P., Miyawaki, A., Jackson, I.J., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP]
reference Ford, M.J., A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice. Dev Cell 2018 Nov 19;47(4):509-523.e5. (2018). PMID 30458140. [link to RRC of NBRP]
reference Bertero, A., Fucci2a mouse upgrades live cell cycle imaging. Cell Cycle 14 (7): 948-949 (2015). PMID 25807308.
reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.
user_report Murganti, F., FUCCI-Based Live Imaging Platform Reveals Cell Cycle Dynamics and Identifies Pro-proliferative Compounds in Human iPSC-Derived Cardiomyocytes. Front. Cardiovasc. Med. 9: 840147 (2022). PMID 35548410. [link to RRC of NBRP]
user_report Ducloux, C., Enhanced Cell-Based Detection of Parvovirus B19V Infectious Units According to Cell Cycle Status. Viruses 12 (12): 1467 (2020). PMID 33353185. [link to RRC of NBRP]