RDB14127 - pDll1-Ub-Fluc

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pDll1-Ub-Fluc (#RDB14127)

Reporter vector for time-lapse imaging of Dll1 transcription.

Clone info.	Reporter vector for time-lapse imaging of Dll1 transcription. This construct contains Dll1 promoter and destabilized luciferase cDNA.
Comment	Expression was confirmed by the depositor with bioluminescence imaging. See Fig.1 of Shimojo, H. et al., Genes Dev. 30 (1): 102-116, 2016.
Vector backbone	pBluescript (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Dll1 genomic DNA firefly (Photinus pyralis) cDNA
Depositor\|Developer	Kageyama, Ryoichiro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14127	pDll1-Ub-Fluc	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDll1-Ub-Fluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14127).

Reference section:

Shimojo, H., Isomura, A., Ohtsuka, T., Kori, H., Miyachi, H., Kageyama, R., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14127_A6Djp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB14127_A6Dja.seq
>D03343A1_A6Dj_2_M13_-40_E03_15.ab1 1 CCTGCACAGG GATTGTATAC GACTCACTAT AGGGCGAATT GGGTACCGGG CCCCCCCTCG 61 AGGTCGACGC GGCCGCTTGT AGTTCATTAT ATTTATTTTG GAAAAATATT TTTAAAATGA 121 ATTTCTTCAT TAACAAAACA GTAAAAAACT CAAATAACAT TTGCACAATA TTCCATAAAT 181 ACATTTATAT ACAAAAAAAT ATAGTCACAT AGACCCGAAG TGCCTTTGTA CATATTTACC 241 AAAATTTAAA TTATAAAAAA ATGAACGTCA CAAAATACTC AAGCTTTACA GATATCATGA 301 AAAATATTTT TACAAATCCC AAAAAAATAA CACACGTTTT CTTTTTCCCG TCTAGGAAAA 361 AAAAAAACAC ATCTCAGTAT CAAAAAGGAC AATAAAGGCA GTTGTGTTTC TAGTTCAAGG 421 AAAGACTGGC TCATAGTAAT CCAAGATAGA CGTGTGGGCA GTGCGTGCTT CCTATATGAA 481 TCAAGTCACT CGTCCATTTA AATATATACT CTTCAAAGCA ACTGTCCATA GTGCAATGGG 541 AACAACCAGC AGGCAGTCCA GCGGCAGCCA AAGCCTAGGC TGGCAGTGTC GGGCGCCTCT 601 GCTAACTCTG AGAGAACCAG CTTCGCCTGA ACCTGGTTCT CAGCAGCAGT CCCTGGGTTT 661 CCTCTCCCTT CCTCTCTCAG CAGCATTCAT CGGGGCTATA TCCTTGGAAT TTTATTTAAG 721 AGAAATGGGA ATTTTGCCAC ATCGCTTCCA TCTTACACCT AGAAGGGGTC GACGGTATCG 781 ATGTGAGGTT ACACGGCGAT CTTTCCGCCC TTCTTGGCCT TTATGAGGAT CTCTCTGATT 841 TTTCTTGCGT CGAGTTTTCC GGTAAGACCT TTCGGTACTT CGTCCACAAA CACAACTCCT 901 CCGCGCAACT TTTTTCGCGG TTGTTACTTT GACTGGCGAC GTAATCCACG ATCTCTTTTT 961 TCCGTCATCG TCTTTCCGTG CTCCCAAAAC AACAACGGCG GCGGGAAAGT TTCACCGGCG 1021 TCATCGTCGG AAGACTGCGA CACCTGCGTC GAAGATGTGG GGTGGTTGGA GCAGGATTGA 1081 TCCGAATCAG CGGGAAGCCA CCTTGATAGC CTGTTTTGTT ACCTA //
Primer: Reverse2 Sequence file: RDB14127_A6Djb.seq
>D03343A1_A6Dj_2_Reverse2_H10_22.ab1 1 ATACCGGGGA ATTACGGCCC AAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG 61 GAGCTCCACC GCGGTGGCGG CCGCTCTAGA ACTAGTCCTG ATACCAGGTG AGGTGAGAGC 121 ACTGTGGCCC GGGCCCAGCT GCATGGAACC AGCACGACTT CGTCTCAAAG CTTTGTGACT 181 CAAGTCGTAC CCACATCCAC AGGCCCAGCG ATGGCCACCT CTCTAGCACC GCCACCGTCC 241 AGAAGCCCCC AGGGGCGCTA ACTCTAGTTG AGAGTCAGAA ACGTATGAGC AAACTAAACG 301 CAACCCCTCC CCTGTTAGAG CCTTCTTCCC ACAAACCCCT CACCCCATTG CTACCCATTC 361 CAGACCAAAG GCCGCGGAAA GTTCCGAGCG AGGTGCACGC GTGACTCTCT TCGGCTGGCC 421 ACGAGCAGCT GCTAGGATAA TCTGATCCTG GGACAAAGGC CCGCCCGCCC GGGCTGCAGC 481 CGCAGGTAAA CACGCGCTTT GTTATGGAAA TCAGCGCAGG ACAGCCCGGA GCAGACAACC 541 CACGATTCCG AGGAAGGAGA GGCATAGGGG GAGGGGCCTC GGGTGGGGCG ATCTGTCATC 601 TGCGCGGCAG CCGCCAGCTG AGCCACGCGC CTCCCCTTTT CTCATGTAAA TCAGCACATG 661 CCTTTGCAAC TTTCTGCAGC CGGCTTCCCA AGACCCACGC ACCCCAGGGT GAGGTGTCCT 721 CGGAGCGCAT ACAATGGCTC TGAGTTCCTA TCTAGCTACC CAAAGGAAAA TCACCAACTA 781 GGTGGGTAGA AGGAGGCGGC AGCCGGTTCC ATCAAAGAAG AAAGGGCTGT GATCCTACGG 841 CATGACGGAG TTCGGACCCC GATCCACTCT GAAGTTCACG GGGCGCCATG CCGGAGTCTT 901 TTGGGTTCAT TACTCCTTTT TGAGTGTACT GCCCACAGAA CGCAGAGGAG GCTAGGCCTA 961 CCGGTTCCAC CTGGTGGGTG TGAATCTGAA GGCTCGAAGT CTCTAGTCTA ATTGGAAAGC 1021 CAAATGCCTG AATTGCATTC AAGTGTGCAC ATGGTCTGTA CAACCATTAA GTGTGTGCGC 1081 CAAGTATAAT GGGTTGGCAT GGCCTTTGTA A //
Primer: Luc_R Sequence file: RDB14127_A6Djc.seq
>D03343A1_A6Dj_2_Luc_R_B01_04.ab1 1 CATATGCTTC AAGGAACAGA GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG 61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCAA 121 GCTTACTAGT CACCACACCT CTGAGACGGA GTACCAGGTG CAGGGTGGAC TCTTTCTGGA 181 TGTTGTAGTC AGACAGGGTG CGCCCATCTT CCAGCTGTTT TCCAGCAAAG ATCAGCCTCT 241 GCTGGTCAGG AGGGATGCCT TCCTTATCTT GGATCTTTGC CTTGACATTC TCAATGGTGT 301 CACTGGGCTC AACCTCGAGG GTGATGGTCT TACCAGTCAG AGTCTTCACG AAGATCTGCA 361 TGGTGGCGGC GGTTACCTTA ACATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG 421 ATCCACTAGT CCGCTGGACG CCCTTGCTCT CCTAGAGTAT CCTCTTTTCT TTCTTCTTCA 481 AAGACCCAGG GATGCCAGGA AAGTCTATAT GACCCGAGGA GTACTGGCGG AAAGGCGTCT 541 CTCGGCTCCA GGTGTGCTAT CAGGGCCAGG TCGCTTCAGG AGGGTGCGGT GCCTTCAGCT 601 AGGTCCCCGG CAAGAGCTCT ACGAGGCACT GAGAAAGGAC AACTTTCGGT TTCCTCTTCG 661 TCTGGCTTTC AGTCCACGGA GAGGTGAGTG TCTCCTGCTT TCTGGTTTTG TCTTAAGCTT 721 CTTCGCGGAG GGAAAGAGGA GAAAGGAGAT AAGACACAGA GGGAAGAGAG CCCAGATGTT 781 CAGCTTAATT CCCAAGAGAG CTTGCAGTGG CTTCCTTCGC AGGCCTATTA GGAAGCGGCG 841 GTCTGGAAAT CCCACCGGTG AGGCGATAAT CCGGGGCCCC GAGGGGCGCG GTGGGAACGG 901 TGGTGGCAAG GACTGCGGCG CGCGCCCTGG TTTCCCGGCC CGGCGTCACT CGGCAGCCCG 961 CTGCCGCTCC CAGAGTGTTC TAGACCGGGT ATCTCTTGCA CTATCGCCAG CCTCGCGTCT 1021 TTCCCTCAAG AATTTCAGCC CAATGCCCAT GCGAGACACA CACCGAAGAA CCGGAGCCCG 1081 AAAGCGTGGG AAGCTCGTTG CCGCACCGGG TGCTTCTTCC CCGGTGTAAT CT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556. [link to RRC of NBRP]