Marker of cholesterol-rich domain of the plasma membrane. Bacterial expression plasmid of His6-mCherry-tagged perfringolysin theta toxin D4 of C. perfringens.
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External Database Clostridium perfringens perfringolysin O (pfoA) Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)). |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link] |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。 |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB14300 | pET28/His6-mCherry-D4 | DNA solution |
Materials & Methods section:
The pET28/His6-mCherry-D4 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14300). |
Reference section:
Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.G., Kasai, R.S., Kubo, S., Schieber, N.L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton R.G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Nakanori that specifically binds to a complex of sphingomyelin and cholesterol. (English text) |
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Featured content | Nakanori that specifically binds to a complex of sphingomyelin and cholesterol. (Japanese text) |
Featured content | Visualization of Organelles (English text) |
Featured content | Sphingolipid signaling pathway (English text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB14300_A6Hup1-4.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: T7_20bp (Pr0261) Region: 6xHis,thrombin site,insert 5' (mCherry 5') Sequence file: RDB14300_A6Hud.seq |
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>D03762A1_A6Hu_3_T7_20bp_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTTNNNNNNN NNNNNNNNNA GGAGANATAC 61 CATGGGCAGC AGCCATCATC ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCGG 121 TACCATGGTG AGCAAGGGCG AGGAGGATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT 181 CAAGGTGCAC ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA 241 GGGCCGCCCC TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT 301 GCCCTTCGCC TGGGACATCC TGTCCCCTCA GTTCATGTAC GGCTCCAAGG CCTACGTGAA 361 GCACCCCGCC GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA 421 GCGCGTGATG AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA 481 GGACGGCGAG TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC 541 CGTAATGCAG AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA 601 CGGCGCCCTG AAGGGCGAGA TCAAGCAGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA 661 CGCTGAGGTC AAGACCACCT ACNAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA 721 CGTCAACATC AAGTTGGACA TCACCTCCCA CNANCGAGGA CTACACCATC GTGGAACAGT 781 ACGAACGCGC CGAGGGCCGC CNCTCCANCG GCGGCATGGA CNAGCTGTAC AAGANNCTTA 841 AGGGAAAAAT NAACTTAGAT CATANTGGAG CCTATGNTGC ACAGTTTGAA GTANCNTGGN 901 NNNGANGTTT CATATGACNA AGANNNAATG AANNTTTANN TCNTAAACAT GGGATGNNAA 961 TTATCNNGAN AANACAGCTC ACTANTNNNA CNNTNNNNCC NCNTGAANCT NATGNNANNN 1021 NNNNAANNNN NAANNCNNGA NNNNNNNCNN NNCNNNNCNT NNNNNNNNGN NNNNNNNNN // |
Primer: T7 terminator (Pr0155) Region: insert 3'(D4,mCherry 3') Sequence file: RDB14300_A6Hue.seq |
>D03762A1_A6Hu_3_T7 Terminator_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGNTTNNTN NGCAGCCGGN NCTCNGTGGT GGTGGTGGTG 61 GTGCTCGAGT TAATTGTAAG TAATACTAGA TCCAGGGTAT AAAGTTGTTC CCCATATTGA 121 AACATTTATA TTATTTGTTA ATGGAACATC ATATTCACTT ATAACATCTC TCCACCATTC 181 CCAAGCAAGG CCTGTACACT CTCTTGCTTT TATTCTTATA TTTCTTGCAT TAGCTTCAAG 241 AGGTATTACT GTTGAATAGT GAGCTGTTTT ATCTTGATAA TTTCCATCCC ATGTTTTATG 301 AGTTAAAACT TCATTTCCTT CTTTGTCATA TGAAACTTCA TCCCAGGCTA CTTCAAACTG 361 TGCAACATAG GCTCCACTAT GATCTAAGTT TATTTTTCCC TTAAGCTTCT TGTACAGCTC 421 GTCCATGCCG CCGGTGGAGT GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA 481 GTCCTCGTTG TGGGAGGTGA TGTCCAACTT GATGTTGACG TTGTAGGCGC CGGGCAGCTG 541 CACGGGCTTC TTGGCCTTGT AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT 601 CAGCTTCAGC CTCTGCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC 661 GGAGGAGGCC TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG ANGGGAAGTT 721 GGTGCCGCGC AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCANGGANG ANTCCTGGGT 781 CACGGTCACC ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA AGNNNTCGGG 841 GAAGGACAGC TTCAAGTANT CGGGGANGTC NGCGGGGNGN TTCACGTANG CNTTNGNANN 901 NGTACATGAN CTNANGGGGA NAGGANGTCC CANNCGAANG NNANGGGGCC ACCCTNNGNC 961 ANNNNCNNCN TNNNNNNCNG GGNGNCCNNN NNNGNNNNNN NNNN // |
Primer: mCherry-N_rev (Pr0398) Region: thrombin site,6x His,RBS,lacO, T7 pro,lacI pro,lacI Sequence file: RDB14300_A6Huf.seq |
>D03762A1_A6Hu_3_mCherry-N_rev_A02_02_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNGN NGGNNNTGTT ANCCTCCTCG CCCTTGCTCN CCATGGTACC 61 GCTGCCGCGC GGNACCAGGC CGCTGCTGTG ATGATGATGA TGATGGCTGC TGCCCATGGT 121 ATATCTCCTT CTTAAAGTTA AACAAAATTA TTTCTAGAGG GGAATTGTTA TCCGCTCACA 181 ATTCCCCTAT AGTGAGTCGT ATTAATTTCG CGGGATCGAG ATCTCGATCC TCTACGCCGG 241 ACGCATCGTG GCCGGCATCA CCGGCGCCAC AGGTGCGGTT GCTGGCGCCT ATATCGCCGA 301 CATCACCGAT GGGGAAGATC GGGCTCGCCA CTTCGGGCTC ATGAGCGCTT GTTTCGGCGT 361 GGGTATGGTG GCAGGCCCCG TGGCCGGGGG ACTGTTGGGC GCCATCTCCT TGCATGCACC 421 ATTCCTTGCG GCGGCGGTGC TCAACGGCCT CAACCTACTA CTGGGCTGCT TCCTAATGCA 481 GGAGTCGCAT AAGGGAGAGC GTCGAGATCC CGGACACCAT CGAATGGCGC AAAACCTTTC 541 GCGGTATGGC ATGATAGCGC CCGGAAGAGA GTCAATTCAG GGTGGTGAAT GTGAAACCAG 601 TAACGTTATA CGATGTCGCA GAGTATGCCG GTGTCTCTTA TCAGACCGTT TCCCGCGTGG 661 TGAACCAGGC CAGCCACGTT TCTGCGAAAA CGCGGGAAAA AGTGGAAGCG GCGATGGCGG 721 AGCTGANTTA CATTCCCAAC CGCGTGGCAC AACAACTGGC GGGCAAACAG TCGTTGCTGA 781 TTGGCGTTGC CACATCCAGT CTGGCCCTGC ACGCGCCGTC GCAAATTGTC GCGGCGATTA 841 AATCTCGCGC CGATCAACTG GGTGCCAGCG NGGTGGNGTC GATGGNAGAA CGAAGCGGCG 901 TCNAAGCCTG NAAAGCGGCG GNGCACAATC TTCTCGCGCA ACGCGNCAGN NGGCTGATCN 961 TNNANNATCC NCNNGNATNA NNNNNNNGNN NNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [link to RRC of NBRP] |
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reference | Abe, M., Imaging cholesterol depletion at the plasma membrane by methyl-β-cyclodextrin. J. Lipid Res. 62:100077 (2021). PMID 33891936. |
user_report | Miyata, Y., Protocol to analyze lipid asymmetry in the plasma membrane. STAR Protoc. 3 (4): 101870 (2022). PMID 36595929. [link to RRC of NBRP] |
user_report | Kitano, V.J.F., LDL uptake-dependent phosphatidylethanolamine translocation to the cell surface promotes fusion of osteoclast-like cells. J. Cell Sci. 133 (10): jcs243840 (2020). PMID 32295848. [link to RRC of NBRP] |
2024.03.26
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