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pET28/His6-EGFP-Nakanori (#RDB14301)

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori.

Clone info. Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of His6-EGFP-tagged G. frondosa Nakaniori. Nakanori is non-toxic mushroom protein that specifically binds a complex of sphingomyelin.
Comment Expression was confirmed by the depositor with SDS-PAGE and confocal micriscopy. AB700589.1 for Grifola frondosa Nakanori mRNA for hypothetical protein, complete cds.
Vector backbone pET-28b (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kan^r
Gene/insert name Grifola frondosa Nakanori cDNA
Depositor|Developer Sako, Yasushi | Abe, Mitsuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino, A. et al., FASEB J. 31 (4): 1301-1322 (2017))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14301 pET28/His6-EGFP-Nakanori DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET28/His6-EGFP-Nakanori was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14301).

Reference section:

Makino, A., Abe, M., Ishitsuka, R., Murate, M., Kishimoto, T., Sakai, S., Hullin-Matsuda, F., Shimada, Y., Inaba, T., Miyatake, H., Tanaka, H., Kurahashi, A., Pack, C.G., Kasai, R.S., Kubo, S., Schieber, N.L., Dohmae, N., Tochio, N., Hagiwara, K., Sasaki, Y., Aida, Y., Fujimori, F., Kigawa, T., Nishibori, K., Parton R.G., Kusumi, A., Sako, Y., Anderluh, G., Yamashita, M., Kobayashi, T., Greimel, P., Kobayashi, T., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14301_A8Gop1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp (Pr0261)
Region: 6XHis,thrombin site,EGFP,insert5'
Sequence file: RDB14301_A8Goa.seq check
>14301_14301_A8Go_T7_20bp_A02_02_ABI24.ab1
    1 TTCCAAGATC AATTTCGCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACG
   61 CATGGGCAGC AGCCATCATC ATCATCATCA CAGCAGCGGC CTGGTGCCGC GCGGCAGCCA
  121 TATGGCTAGA GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA
  181 GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC
  241 CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG
  301 GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA
  361 CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC
  421 CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA
  481 CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT
  541 GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA
  601 GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA
  661 GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA
  721 CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA
  781 CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA
  841 CAAGAAGCTT GGAGGAGGAG GAGGAGAATT CATGCTTTAC GGCGTCGAGA TAGACGAGCA
  901 GTACCTGCGC GTGATGGAGG AGTACAAGGA CAAGGAAAGT AATCACGCAG GCCGACATGG
  961 CCAAGGTCGC GCTACAACGC AAGAACGTGT ACCAGGACCA GGCCGAGGAA GCGCCAGGCT
 1021 GAGCTTAAAG GCCCGAAATA TGGGAGTCGG TGTCTTGTGT CCTCGTGCCG TGTTGTTACA
1081 ATTGCTACTG GGTCGGATCC CCCGAATC
//
Primer: T7 terminator (Pr0155)
Region: insert3'
Sequence file: RDB14301_A8Gob.seq check
>14301_14301_A8Go_T7_terminator_B02_05_ABI24.ab1
    1 GAATGCTTCT TTCGGGCTTT GTTAGCAGCC GGATCTCAGT GGTGGTGGTG GTGGTGGGAT
   61 CCTCAACGGG AGTACACATA CTCCACGTAA GCGGTAGTCT CATTTCCAGT CGACCCGTGA
  121 GCCTTGACAT CGCCGTAGCT CGAGTCGCTA TTCTGGCCGT TGTGCGTGTC GAGTAGGTGG
  181 AGCATATAGT CCTTGCTACC GTTCTTCCAC CAATGACCGC TCTGGCGCAC CTCAACGAGC
  241 ACCTGAGAAT CCCACGAGCG GTTCCAGGGG TTCTGCCAGC CTGAGAAGAT GTCGAGGTTG
  301 TCCGTGGTGC CATACACGAT GCACCCCGCG GACCCGACTG CGGCGCCCGC CGACTTGGTG
  361 TGGATGAACA CCGTCCATTG CCCATTCCCG ATGCGCTTTT CTCGAGTGTG CGCGCCAAAG
  421 TGGCCTCGGA AGGACTCCTC GATCTTCGCT GTGATCGGGC CGCCAGTAGC ATTGTACACA
  481 CGCACGAGGA CACAGACACC GACTCCATAT TCGGCCTTAA GCTCAGCCTG GCGCTTCTCG
  541 GCCTGGTCCT GGTACACGTT CTTGCGTTGT AGCGCGACCT TGGCCATGTC GGCCTGCGTG
  601 ATTACTTCCT TGTCCTTGTA CTCCTCCATC ACGCGCAGGT ACTGCTCGTC TATCTCGACG
  661 CCGTAAAGCA TGAATTCTCC TCCTCCTCCT CCAAGCTTCT TGTACAGCTC GTCCATGCCG
  721 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG
  781 GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG
  841 CCGTCGCCGA TGGGGGGTGT TCTGCTGGTA GTGGTCGGCG AGCTGCACGC TGCCGTCCTC
  901 GATGTTGTGG GCGGGATCTT GAAGGTTCAC CTTTGATGCC GTTCTTTCTG CTTTGTCGGG
  961 CCATGATATA AGACGTTGTG GCTGTTTGTA GGTGTACTCC AAGCTTGTGC CCCCAGGATG
 1021 GTTGCCGGTC CTCCATGGAA GTTCGAATGG CCCTTCCAGC TCGAATGCGG TCACAAGGTG
1081 TTCGACCTCG CACCTCACCT CCGGCGCGCG GGGACTA
//
Primer: lacIpro_F3 (Pr0722)
Region: lacI pro,lacI 5'
Sequence file: RDB14301_A8Goc.seq check
>14301_14301_B0CcS_lacIpro_F3_H04_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCTGGGC TGCTTCCTAA TGCAGGANTC GCATAAGGGA
   61 GAGCGTCGAG ATCCCGGNCA CCATCGAATG GCGCAAAACC TTTCGCGGTA TGGCATGATA
  121 GCGCCCGGAA GAGAGTCAAT TCAGGGTGGT GAATGTGAAA CCAGTAACGT TATACGATGT
  181 CGCAGAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC GTGGTGAACC AGGCCAGCCA
  241 CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG GCGGAGCTGA ATTACATTCC
  301 CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG CTGATTGGCG TTGCCACCTC
  361 CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG ATTAAATCTC GCGCCGATCA
  421 ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC GGCGTCGAAG CCTGTAAAGC
  481 GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG ATCATTAACT ATCCGCTGGA
  541 TGANCAGNAT GCCATTGCTG TGNAAGCTGC CTGCACTAAT GTTCCGGCGT TANTTCNTGA
  601 TGTCTCTGAN NNGACACCCN TCNACAGTAN NANTTTCNCC CATGANNANN GNACNCGANN
  661 NNNNTNGNNC ATCTGGNCNN ATTNNNNCNN NNNCAAATCG CGCTNNNNNC GGNNNNNTTN
721 NGNNCNGNNT CNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Makino, A., A novel sphingomyelin/cholesterol domain-specific probe reveals the dynamics of the membrane domains during virus release and in Niemann-Pick type C. FASEB J. 31 (4): 1301-1322 (2017). PMID 27492925. [link to RRC of NBRP]