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R-CaMP1.07 (#RDB14608)

Genetically-encoded fluorescent calcium sensor cDNA, R-CaMP1.07.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ohkura, M., PLoS One 7 (7): e39933 (2012). PMID 22808076. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of mApple, calmodulin, and the M13 peptide from myosin light chain kinase. By combining this red fluorescent calcium sensor with the green fluorescent calcium sensor, such as G-CaMPs became possible to observe the relationship between two different neural networks.
Comment Coding sequence of EGFP was replaced with R-CaMP1.07 sequence. Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Growth conditions LB+Kanamycin, 37oC
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AKE44627.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |

Distribution information

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Terms and conditions for distribution In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (7): e39933, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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MTAに書く使用条件 研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (7): e39933, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

Catalog # Resource name Shipping form Fee
RDB14608 R-CaMP1.07 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The R-CaMP1.07 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14608).

Reference section:

Ohkura, M., Sasaki, T., Kobayashi, C., Ikegaya, Y., Nakai, J., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076. [PubMed] [Article] [RRC of NBRP]
reference Bethge, P., An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator. PLoS One 12 (6): e0179460 (2017). PMID 28640817. [PubMed] [Article]
reference Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959. [PubMed] [Article]