RDB14610 - G-CaMP7

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G-CaMP7 (#RDB14610)

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP7.

Clone info.	Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP7 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Comment	Expression was confirmed by the depositor with calcium imaging.
Vector backbone	pEGFP-N1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name	Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	KP230402.1 (DDBJ accession number)
Depositor\|Developer	Nakai, Junichi \| Ohkura, Masamichi \|

Distribution information

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Terms and conditions for distribution In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14610	G-CaMP7	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The G-CaMP7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14610).

Reference section:

Ohkura, M., Sasaki, T., Sadakari, J., Gengyo-Ando, K., Kagawa-Nagamura, Y., Kobayashi, C., Ikegaya, Y., Nakai, J., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14610_A7Bgp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5' Sequence file: RDB14610_A7Bgd.seq
>14610_14610_B2IuF_CMV_Forward_B04_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGG GCNGNANGCG TGNACNGNGG GAGGNCTATA 61 TNAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC 121 CACCATGCGG GGTTCTCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA 181 AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC 241 ACGTCGTAAG TGGAATAAGA CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT 301 CGAGAACGTC TATATCAAGG CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT 361 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC 421 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCGTTC AGAATAAACT 481 TTCGAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC 541 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG 601 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 661 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT 721 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC 781 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCANCACGA 841 CTTCTTCNAG TCCGCCNTGC NNGANNGCTA CATCCAGGNN CGCACCATCT TCTTCAAGGA 901 NGACNGNNAC TACAAGANCC NCGCCNNNGN GAAGTTCGAG GGNGANACCN NGNNNAANCN 961 CATCNNGCTN NANNNNNATC NNNTTCNNNN ANGNNNNNNN NNN //
Primer: SV40polyA-R (Pr0109) Region: insert 3' Sequence file: RDB14610_A7Bge.seq
>D04021A5_A7Bg_1_SV40polyA-R_E11_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNN TCTANNAATG TGGTATGGCT GATTATGATC 61 TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG 121 ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT 181 CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC 241 CTTATCAAAC ACACGGAACG CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT ATTTCATTTT 301 TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCATCACCGT CGGCATCTAC 361 TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCAGCAC 421 CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC 481 CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT 541 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCACGA TGCGGTTCAC 601 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTAGCCGT CGTCCTTGAA 661 GAAGANGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG 721 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TNNNCAGGGT GGTCACGAGG 781 GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCANATGA ACTTCANGGT CAGCTTGNCG 841 NANGTGGNAT CGCCCTCNCC CTCGCCNGAN ACGCTGAACT TGTGGNCGTT TANGTCGCCN 901 TCCANCTCGA NCANGNNGGG NACCACCCCN GNGAANNGCT CCTCGCCCTT GCTCNNNNNG 961 CTNCCNNCNG NNACNNCCCT TNNNCAGCTC NNNCNNNNNN ANNNTGNTCC NNGNNNNNNG 1021 NNNNNNNNNT NNNNNNNNNN //
Primer: Venus-C_F2 (Pr0454) Region: insert mid Sequence file: RDB14610_A7Bgf.seq
>14610_14610_B2IuF_Venus-C_F2_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTC NNNNNGGNCG AGCTGTACNA GGGCGGTACC 61 GGAGGGAGCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC 121 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG TGAGGGCGAT 181 GCCACCTACG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC 241 TGGCCCACCC TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC 301 CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACAT CCAGGAGCGC 361 ACCATCTTCT TCAAGGACGA CGGCTACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC 421 GACACCCTGG TGAACCGCAT CGTGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC 481 CTGGGGCACA AGCTGGAGTA CAACACGCGT GACCAACTGA CTGAAGAGCA GATCGCAGAA 541 TTTAAAGAGG CTTTCTCCCT ATTTGACAAG GACGGGGATG GGACAATAAC AACCAAGGAG 601 CTGGGGACGG TGCTGCGGTC TCTGGGGCAG AACCCCACAG AAGCAGAGCT GCAGGACATG 661 ATCAATGAAG TAGATGCCGA CGGTGATGGC ACAATCGACT TCCCTGAGTT CCTGACAATG 721 ATGGCAAGAA AAATGAAATA CACAGACAGT GAAGAAGAAA TTAGAGAAGC GTTCCGTGTG 781 TTTGATAAGG ATGGCAATGG CTACATCAGT GCAGCAGANC TTCGCCACGT GATGACNAAC 841 CTTGNAGAGA AGTTAACAGA TGAAGANGTT GATGAAATGA TCAGGGAAGC AGACATCGAT 901 GGGGATNGTC ANGTAANCTA CNAAGANTTT GTACAAATGA TGACAGCGAA NTGAGCGNNC 961 GCNNNTCTAG ATCATAANCA NCNNTANCNN ATTTGNAGAN GNTTTACNTN NNTTNNAAAA 1021 ANNNNNCNNN NNNNNCCNNN NNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB14610_A7Bgg.seq
>D04021A5_A7Bg_1_SV40pro_F_V2_E12_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT GNNNAGNNCG ATCAAGAGAC AGGANGAGGN 61 NCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG 121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC 181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG 241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC 301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG 361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT 421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG 481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT 541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC 601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG 661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGANCGC 721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT 781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT 841 CGCCTTCTTG ACGAGTTCTT CTGANCGGGA NTCTGGGGTN CGAAATGANC GANCNAGCGA 901 CGCCCAACCN GCCNTCNCGA GATTTCGANN CCNCCGCCNC CTTCTATNAA AGGNTGGNNT 961 TCNGNNNCGT TTTNNNGGNN NCNNGCNNGN TGNNNCNTNN NNCNNGGGGN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011. [link to RRC of NBRP]
reference	Monai, H., Calcium imaging reveals glial involvement in transcranial direct current stimulation-induced plasticity in mouse brain. Nat. Commun. 7: 11100 (2016). PMID 27000523.
reference	Sato, M., Generation and Imaging of Transgenic Mice that Express G-CaMP7 under a Tetracycline Response Element. PLoS One 10 (5): e0125354 (2015). PMID 25946002.
review	Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.