RDB14611 - G-CaMP8

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G-CaMP8 (#RDB14611)

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP8.

Clone info.	Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP8 is high-sensitivity and fast-responsivity calcium sensor that reliably monitor neural spikes with larger fluorescence signals and higher temporal resolution.
Comment	Expression was confirmed by the depositor with calcium imaging.
Vector backbone	pEGFP-N1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name	Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence	AKE44626.1 (DDBJ accession number)
Depositor\|Developer	Nakai, Junichi \| Ohkura, Masamichi \|

Distribution information

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Terms and conditions for distribution In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (12): e51286, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (12): e51286, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14611	G-CaMP8	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The G-CaMP8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14611).

Reference section:

Ohkura, M., Sasaki, T., Sadakari, J., Gengyo-Ando, K., Kagawa-Nagamura, Y., Kobayashi, C., Ikegaya, Y., Nakai, J., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14611_A7Bgp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,5xHis,T7 tag,Xpress tag,insert 5' Sequence file: RDB14611_A7Bga.seq
>D04021A6_A7Bg_1_CMV-Forward_F10_16_ABI24.ab1 1 TGGTTTCAAC TCCGCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC 121 CACCATGGGT TCTCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT 181 GGGTCGGGAT CTGTACGACG ATGACGATAA GGATCTCGCC ACCATGGTCG ACTCATCACG 241 TCGTAAGTGG AATAAGACAG GTCACGCAGT CAGAGCTATA GGTCGGCTGA GCTCACTCGA 301 GAACGTCTAT ATCAAGGCCG ACAAGCAGAA GAACGGCATC AAGGCGAACT TCAAGATCCG 361 CCACAACATC GAGGACGGCG GCGTGCAGCT CGCCTACCAC TACCAGCAGA ACACCCCCAT 421 CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG AGCGTTCAGA ATAAACTTTC 481 GAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG GAGTTCGTGA CCGCCGCCGG 541 GATCACTCTC GGCATGGACG AGCTGTACAA GGGCGGTACC GGAGGGAGCA TGGTGAGCAA 601 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA 661 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG TGAGGGCGAT GCCACCTACG GCAAGCTGAC 721 CCTGAAGTTC TTCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC 781 CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT 841 CTTCAAGTCC GCCATGCCCG AAAGCTACAT CCAGGAGCGC ACCATCTTCT TCAAGGACGA 901 CGGCTACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT 961 CGTGCTGAAG GGCATCGACT TCAAGGAGAC GGCAACATCC TGGGGGCACA AGCTGGAAGT 1021 ACACACGCGT GACCAACTGA CTGGAGAGCA GATCGCAGAT TAAGAGCTTT TCCTCCTATT 1081 TGACAGGACG GAATGACAAT ACCAACAAGA AGCTGGGAGC ACGGTGTGCT TGC //
Primer: SV40polyA-R (Pr0109) Region: insert 3' Sequence file: RDB14611_A7Bgb.seq
>D04021A6_A7Bg_1_SV40polyA-R_F11_17_ABI24.ab1 1 AAGAAATGGG AGTTTTTAAG CAGTAAAACC TCTACAAATG TGGTATGGCT GATTATGATC 61 TAGAGTCGCG GCCGCTCACT TCGCTGTCAT CATTTGTACA AACTCTTCGT AGTTTACCTG 121 ACCATCCCCA TCGATGTCTG CTTCCCTGAT CATTTCATCA ACCTCTTCAT CTGTTAACTT 181 CTCTCCAAGG TTTGTCATCA CGTGGCGAAG CTCTGCTGCA CTGATGTAGC CATTGCCATC 241 CTTATCAAAC ACACGGAACG CTTCTCTAAT TTCTTCTTCA CTGTCTGTGT ATTTCATTTT 301 TCTTGCCATC ATTGTCAGGA ACTCAGGGAA GTCGATTGTG CCATCACCGT CGGCATCTAC 361 TTCATTGATC ATGTCCTGCA GCTCTGCTTC TGTGGGGTTC TGCCCCAGAG ACCGCAGCAC 421 CGTCCCCAGC TCCTTGGTTG TTATTGTCCC ATCCCCGTCC TTGTCAAATA GGGAGAAAGC 481 CTCTTTAAAT TCTGCGATCT GCTCTTCAGT CAGTTGGTCA CGCGTGTTGT ACTCCAGCTT 541 GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC TTCAGCACGA TGCGGTTCAC 601 CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTAGCCGT CGTCCTTGAA 661 GAAGATGGTG CGCTCCTGGA TGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG 721 CTTCATGTGG TCGGGGTAGC GGCTGAAGCA CTGCACGCCG TAGGTCAGGG TGGTCACGAG 781 GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGAAG AACTTCAGGG TCAGCTTGCC 841 GTAGTGGCAT CGCCCTCACC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG 901 TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG 961 CTCCCTCCGG GTACCGCCCT TGTACAGCTC GTCCATGCCG AGAGTGATCC CCGGCGGCGG 1021 TCACGACTCC AGCAGACATG TGATCGCGCT TTCTCGTGGG GTCTTTCGAA GTTTATTTCT 1081 GACGCCTCAG TAATTGATGG TCGGGCAGCA GCACGGTCGT CGTGATGGGG TGATTCTGCC 1141 TGTTATTGTT AGCGAGCTGC ACGTCGC //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB14611_A7Bgc.seq
>D04021A6_A7Bg_1_SV40pro_F_V2_F12_18_ABI24.ab1 1 GGCAGAACTT TTTGGAGCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG 61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 961 GAATCGTTTT CCCGGGACGC CCGGCTGGGA TGATCCTCCA GCGCGGGGAT CTCATGCTGG 1021 GAGTTCTTCG CCACCCTAGG GGGAGCCTAC TGAAACACGG GATGAGACAA TACTGCACGA 1081 CCCGCGCTAT GACGGCATAA AGACCGATTA ACGGCACGGT GTTGGGATCG GTTTGGTTCC 1141 AATAAAACGT CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Ohkura, M., Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. PLoS One 7 (12): e51286 (2012). PMID 23240011. [link to RRC of NBRP]
review	Pastrana, E., Calcium sensors reach new heights. Nat. Methods 10 (9): 824 (2013). PMID 24143827.