Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-T7-GST-Kif1c-tail-879-1100/M-INK

M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
Additional terms and conditions for distribution	Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。
付加的提供条件	pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14634_A7C1p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF1 alpha pro,insert5'(T7 tag,GST,Kif1c 5') Sequence file: RDB14634_A7C1a.seq
>D04384A3_A7C1_2_EF1a-C_F_F02_17_ABI24.ab1     1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT    61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT   121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCTATGTCCC   181 CTATACTAGG TTATTGGAAA ATTAAGGGCC TTGTGCAACC CACTCGACTT CTTTTGGAAT   241 ATCTTGAAGA AAAATATGAA GAGCATTTGT ATGAGCGCGA TGAAGGTGAT AAATGGCGAA   301 ACAAAAAGTT TGAATTGGGT TTGGAGTTTC CCAATCTTCC TTATTATATT GATGGTGATG   361 TTAAATTAAC ACAGTCTATG GCCATCATAC GTTATATAGC TGACAAGCAC AACATGTTGG   421 GTGGTTGTCC AAAAGAGCGT GCAGAGATTT CAATGCTTGA AGGAGCGGTT TTGGATATTA   481 GATACGGTGT TTCGAGAATT GCATATAGTA AAGACTTTGA AACTCTCAAA GTTGATTTTC   541 TTAGCAAGCT ACCTGAAATG CTGAAAATGT TCGAAGATCG TTTATGTCAT AAAACATATT   601 TAAATGGTGA TCATGTAACC CATCCTGACT TCATGTTGTA TGACGCTCTT GATGTTGTTT   661 TATACATGGA CCCAATGTGC CTGGATGCGT TCCCAAAATT AGTTTGTTTT AAAAAACGTA   721 TTGAAGCTAT CCCACAAATT GATAAGTACT TGAAATCCAG CAAGTATATA GCATGGCCTT   781 TGCAGGGCTG GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG GATCTGGTTC   841 CGCGTGGATC TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAAGGG   901 CCAGAAGCAG TAGAGGAGAC AGTGCCCAAC GACCACTCAC CAGCTGTCCG GCCCACCTCA   961 CCACCTCTGT CAGCTGGGAG CGGGGTGTCG AGATTGATGA GAGACCTGCA TCGTCGCGCC  1021 GCATCGCTGC TCAGCAGACA GCTGCGCTGC AAGAACTGCA AGTCGCTGAG CCGATGGGTA 1081 ACTGCCAGGC TTCAGCCCGC GCATTTGA //
Primer: hGCSF_R Region: hG-CSF p(A),insert3'(Kif1c,GST) Sequence file: RDB14634_A7C1b.seq
>D04384A3_A7C1_2_hGCSF_R_E02_14_ABI24.ab1     1 CGGGGGCTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT    61 CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTCACAC AGCTGCCCCA CTCTCTTTGA   121 GGTCAGGGGC TGAGCGCTGC CGTCGCATTC GCGGAGGGGT AGTGTATGGT GGGTAGCGGG   181 GCCCTGGCCG CTGGGCTGGG CTGGGCTGGG GCTGTTGGGG ATAACCGTTG TGCTTTTGGG   241 GCCGTAAGTG CTGGGGTTCT GGCTGTGTAG AACCCCCTCC CCGAGATCGG CTCCCTCCAT   301 CTAGGGAATT CCTGCGAGAA CGGTGGGGTC TTCGGGGACT TGGAGGCCTA CGGTTAGGGG   361 GTGCTGGGGG GACAGTGACC TCTTCAGGGG GCTGAGGAGC TGGGGCCTCT CCACTCCCCA   421 TTCCTGGCCA GGACTCCCTG TGCTGAGGGT TGCTTTTGAA GGGGAAGCGC AGCTTGCAAT   481 CATGCGGGGG CACAAAGCGG GCTGGAGGCC TGCGCAGTCC CCCACCCCGG CCCCCAGCGC   541 CCTGCAGTCC CTGCAGCCGC AGCTGCTCCT GCTTGAGCCA GCGAAGGCGG CCGCGACGGA   601 AGGCAGGGTC CTCTTCCATC AATCTCGACA CCCGCTCCCA GCTGGACAGA GGTGGTGAGG   661 TGGGCCGGAC AGCTGGTGAG TGGTCGTTGG GCACTGTCTC CTCTACTGCT TCTGGCCCTT   721 CCGGTGGGGC CCAGGTGACC AAACCAGACT CGTCATTATC ATCAGATCCA CGCGGAACCA   781 GATCCGATTT TGGAGGATGG TCGCCACCAC CAAACGTGGC TTGCCAGCCC TGCAAAGGCC   841 ATGCTATATA CTTGCTGGAT TTCAAGTACT TATCAATTTG TGGGATAGCT TCAATACGTT   901 TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA   961 ACATCAAGAG CGTCATACAA CATGAAAGTC AGGATGGGTT TACATGATCA CCATTTAAAA  1021 TATGTTTTTA TGACATAAAC GATCTTCGAA CATTTTTCAG CATTTCCAGG TAGCTTGCTA  1081 AGAAAAATCA ACTTTGGAAG GTTCCAAGGT CTTTTTACTA TATGCAAGTT CTTCGGAAAA 1141 ACACCCGTTA TC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl