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pcDNA5-GAPDH-HaloTag (#RDB15088)

Expression vector for the visualization of chaperone-mediated autophagy (CMA).


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Sato, M., Genes Cells 21 (8): 861-873 (2016). PMID 27377049. [PubMed] [Article] [RRC of NBRP]
Publication Seki,T., PLoS One 7 (2): e31232 (2012). PMID 22363588. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name GAPDH-HT/pcDNA5/FRT
Clone info. Expression vector of human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), tagged with C-terminal HaloTag, for genomic integration. Translocation of the GAPDH-HT labeled with an appropriate fluorescent dye as a substrate of chaperone-mediated autophagy (CMA) from cytosol to lysosome can be detected. Refer Fig. 1 of Seki, T., et al., PLoS ONE 7 (2), e31232, 2012.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pcDNA5/FRT (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hyg^r (mammalian cells).
Growth conditions LB+Amp, 37oC
Gene/insert name Human GAPDH cDNA
Depositor|Developer Seki, Takahiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sato, M. et al. Genes Cells 21 (8): 861-873, 2016).
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sato, M. et al. Genes Cells 21 (8): 861-873, 2016)。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB15088 pcDNA5-GAPDH-HaloTag DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA5-GAPDH-HaloTag was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15088).

Reference section:

Sato, M., Seki, T., Konno, A., Hirai, H., Kurauchi, Y., Hisatsune, A., Katsuki, H., Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes Cells 21 (8): 861-873 (2016). PMID 27377049. [PubMed] [Article] [RRC of NBRP]
Seki,T., Yoshino, K.I., Tanaka, S., Dohi, E., Onji, T., Yamamoto, K., Hide, I., Paulson, H.L., Saito, N., Sakai, N., Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron. PLoS One 7 (2): e31232 (2012). PMID 22363588. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Sato, M., Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes Cells 21 (8): 861-873 (2016). PMID 27377049. [PubMed] [Article] [RRC of NBRP]
original Seki,T., Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron. PLoS One 7 (2): e31232 (2012). PMID 22363588. [PubMed] [Article] [RRC of NBRP]