Lentivirus vector plasmid of S/G2/M-R(N).
Clone info. | Lentivirus vector plasmid of S/G2/M-R(N). Fluorescence was detected in the nucleus. |
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Comment | For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008). |
Vector backbone | CSII-EF-MCS (RDB04378) (plasmid) |
Size of vector backbone | 9.1 kb |
Selectable markers | Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Growth conditions | 37oc, LB + Amp |
Gene/insert name | human Geminin cDNA Discosoma sp. RFP cDNA |
Depositor|Developer | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Ordering instruction.[link] |
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Terms and conditions for distribution | 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A. et al., Chemistry & Biology 15: 1243-1248 (2008). Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011). Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. |
Remarks | Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
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MTAに書く使用条件 | 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A. et al., Chemistry & Biology 15: 1243-1248 (2008). Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011). 付加的使用条件: レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。提供条件の言語は英語のみ。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB15269 | mCherry-hGeminin(1/110) / pCSII-EF | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The mCherry-hGeminin(1/110) / pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15269). |
Reference section:
Sakaue-Sawano, A., Ohtawa, K., Hama, H., Kawano, M., Ogawa, M., Miyawaki, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15269_A7H3p1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F Sequence file: RDB15269_A7H3a.seq ![]() |
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>D04498H9-1_A7H3_EF1a-C_F_C01_07_ABI24.ab1 1 TCATTGGAAT TGGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG 61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC TCGAGAATTC 121 GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC 181 TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC 241 GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC 301 CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG 361 AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG 421 GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG 481 CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC 541 CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG 601 GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC 661 GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC 721 AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG 781 TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGGGATAT 841 CCATCACACT GGCGGCCGCT CGAGATGAAT CCCAGTATGA AGCAGAAACA AGAAGAAATC 901 AAAGAGAATA TAAAGAATAG TTCTGTCCCA AGAAGAACTC TGAAGATGAT TCAGCCTTCT 961 GCATCTGGGA TCTCTTGTTG GAAGAGAAAA TGAGCTGTCC GCAGCTTGTC CAAAAGGAAA 1021 CATCGGAATG ACCACTTTAA CATCTACAAC TTCCAGCCCT GGGGGTATGT CCCAGAATCT 1081 AGTGAAAAAT AAAAAATCTG AGAGTCACCC AGGGAGTCAT TGGATCCTAA TGAATCAGAA 1141 ATCAATCTCT CAGTATTGGA AAGGAAGTGG CAGAAAAAAA ACC // |
Primer: PRE_rev Sequence file: RDB15269_A7H3b.seq ![]() |
>D04498H9-1_A7H3_PRE_rev_C02_08_ABI24.ab1 1 ACGTTATCAC ATAGCGTAAA GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA 61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA 121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT 181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC 241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT 301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG 361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC 421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC 481 CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT CGTACTGTTC 541 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC TTGATGTTGA CGTTGTAGGC 601 GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCAG CGTCGTAGTG 661 GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG 721 GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC 781 GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA 841 GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT 901 GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA 961 GGCCTTGGAG CCGTACATGA ACTGAGGGAC AGGATGTCCC AGGCGAAAGG GCAGGGGCCA 1021 CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GGTGCCCCTC GTAGGGGCGC CCTCGCCCTC 1081 GCCCTCGATC TCGAACTCGT ATCGTTCACG GAGCCCTCCA TGGTGCAACT GGAGCGCAAT 1141 GGACTCATGG ATGATGGGCC ATGTTTATCC TTCCCTCGGC CCCTGTGCTT CT // |
Primer: CMV-Forward Sequence file: RDB15269_A7H3c.seq ![]() |
>D04498H9-1_A7H3_CMV-Forward_C03_09_ABI24.ab1 1 TTGTCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT 61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC 121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC 181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT 241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA 301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA 361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA 421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG 481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA 541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT 601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA 661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC 721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT 781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA 841 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT 901 GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGAGCAGCA 961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTTATTGTC 1021 TGGTATAGTG CAGCAGCAGA ACAAATTTGC TGAGGGCTAT TGAGCGCAAC AGCAATCTGG 1081 TGGCAACTCA ACAGGTCTGG GGCATCAAGC AGCTTCAAGG CAGATTCCTG ACTGATGAAA 1141 GAAATACCTT AAAAGGAATT C // |
Primer: BGH_rev2 Sequence file: RDB15269_A7H3d.seq ![]() |
>D04498H9-1_A7H3_BGH_rev2_C04_07_ABI24.ab1 1 CAGGTCGGGT CAGTAGCACG GGGGAGGGGC AACAAGCAGA TGGCTGGCAA CTAGAAGGCA 61 CAGTGCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA 121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG 181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG 241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG 301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG 361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC 421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG 481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG 541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC 601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA 661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC 721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC 781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG 841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG 901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA 961 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT TCCCCGACAA CACCACGGGA ATTTGTCAGT 1021 GCCCAACAGC CCGAGCCCCT GTCCAGCAGC GGGCAAGGCA GGCGGCGATG AGTTCCGCCG 1081 TGCATTAGGA GGGGGAAGCG AAGTCCGGAA AGAGCTGACA GTGTGGCATG CCTCACCAGC 1141 TGGGGGGTTG GGCGGTCCAG GCC // |
Primer: SV40pro_F Sequence file: RDB15269_A7H3e.seq ![]() |
>D04498H9-1_A7H3_SV40pro_F_C05_08_ABI24.ab1 1 CCTGGCCCCG TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA 121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC 181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC 241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC 301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC 361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG 421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG 481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG 541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA 961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT 1021 TAATGAATCG GCCCAACGCG CGGGGGAGAG GCGGTTTGCG TATTTGGGGC GGCTTCTTCG 1081 CTTCTCGCTT CACTTGACTC GCTGCGCTCG ATCGGTTTCG GGCTGCGCCA GCCGGTAATC 1141 AGCTTCACAT GAAGACGGTT AGATACCTGC TGAATCCCTC ACCAG // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468. [link to RRC of NBRP] |
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reference | Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130. [link to Journal] |
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