Lentivirus vector plasmid of Fucci2-G1 phase probe. Component of Fucci2 cell cycle probe.
![]() Drawn by SnapGene® software |
|
Clone info. | Lentivirus vector plasmid of Fucci2-G1 phase probe. One of the component of Fucci2 cell cycle probe. Fluorescence was detected in the nucleus. |
---|---|
Vector backbone | CSII-EF-MCS (RDB04378) (plasmid) |
Size of vector backbone | 9.1 kb |
Selectable markers | Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Growth conditions | 37oc, LB + Amp |
Gene/insert name | human CDT1 cDNA Discosoma sp. RFP cDNA |
Reference of insert sequence | AB512478 (DDBJ accession number) |
Depositor|Developer | Miyawaki, Atsushi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Ordering instruction.[link] |
---|---|
Terms and conditions for distribution | 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011). Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. |
Remarks | Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
---|---|
MTAに書く使用条件 | 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource centers without the prior written consent of DEPOSITOR. 3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested. Sakaue-Sawano, A. et al., BMC Cell Biology 12: 2 (2011). 付加的使用条件: レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。提供条件の言語は英語のみ。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB15273 | mCherry-hCdt1(30/120)/ pCSII-EF | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The mCherry-hCdt1(30/120)/ pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15273). |
Reference section:
Sakaue-Sawano, A., Miyawaki, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473. [link to PubMed] |
Sakaue-Sawano, A., Kobayashi, T., Ohtawa, K., Miyawaki, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression [Japanese text]. The journal of Japanese Biochemical Society (Seikagaku) 84 (1): 47-52 (2012). PMID 22416473. [link to PubMed] |
---|---|
original | Sakaue-Sawano, A., Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biol. 12: 2 (2011). PMID 21226962. [link to RRC of NBRP] |
reference | Singer, D., Prominin-1 controls stem cell activation by orchestrating ciliary dynamics. EMBO J. 38 (2). pii: e99845 (2019). PMID 30523147. [link to Journal] |
reference | Liu, Y., Repair-independent functions of DNA-PKcs protect irradiated cells from mitotic slippage and accelerated senescence. J. Cell Sci. 132 (13). pii: jcs229385 (2019). PMID 31189537. [link to Journal] |
reference | Tan, Z., Mammalian Cells Undergo Endoreduplication in Response to Lactic Acidosis. Sci Rep. 8 (1): 2890 (2018). PMID 29440645. [link to Journal] |
reference | Ohgushi, M., A RHO Small GTPase Regulator ABR Secures Mitotic Fidelity in Human Embryonic Stem Cells. Stem Cell Reports 9 (1): 58-66 (2017). PMID 28579391. [link to Journal] |
reference | Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130. [link to Journal] |
reference | Oki, T., A novel cell-cycle-indicator, mVenus-p27K-, identifies quiescent cells and visualizes G0-G1 transition. Sci. Rep. 4: 4012 (2014). PMID 24500246. [link to Journal] |
reference | Abe, T., Visualization of cell cycle in mouse embryos with Fucci2 reporter directed by Rosa26 promoter. Development 140 (1): 237-246 (2013). PMID 23175634. [link to Journal] |
reference | Nishimura, K., APC(CDH1) targets MgcRacGAP for destruction in the late M phase. PLoS One 8 (5): e63001 (2013). PMID 23696789. [link to RRC of NBRP] |
user_report | Dehghankelishadi, P., Bosutinib high density lipoprotein nanoformulation has potent tumour radiosensitisation effects. J. Nanobiotechnology 21 (1): 102 (2023) PMID 36945003. [link to RRC of NBRP] |
user_report | Badiee, P., Intratumoral Anti-PD-1 Nanoformulation Improves Its Biodistribution. ACS Appl. Mater. Interfaces 14 (14): 15881-15893 (2022). PMID 35357803. [link to RRC of NBRP] |
user_report | Dehghankelishadi, P., Formulation of simvastatin within high density lipoprotein enables potent tumour radiosensitisation. J. Control. Release 346: 98-109 (2022). PMID 35447296. [link to RRC of NBRP] |
Featured content