Please review the QC test results indicated by icon below as well as clone information before placing your order.

tFucci(SA)2.1/pCSII-EF

Lentivirus vector plasmid of mCherry-hCdt1(30/120)_P2A_AmCyan-hGem(1/110). EF-1 alpha promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. Additional terms and conditions: The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Ordering instruction.[link]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

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提供条件	1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Sakaue-Sawano, A., Yo, M., Komatsu, N., Hiratsuka, T., Kogure, T., Hoshida, T., Goshima, N., Matsuda, M., Miyoshi, H., Miyawaki, A. (2017) Genetically-Encoded Tools for Optical Dissection of the Mammalian Cell Cycle. Mol. Cell 68 (3): 626-640.e5. 3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR. 5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD. 付加的提供条件： レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	提供依頼書 [Word] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15444_B1K5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1alpha pro,insert 5'(mCherry 5') Sequence file: RDB15444_B1K5a.seq
>15444_15444_B1K5_3_EF1a-C_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNTTTTGAN TTTGGNNCTT GGTTNNTTCT CAAGCCTCAG ACAGTGGTTC    61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAACACGCTA CCGGTCTCGA GAATTCTGCA   121 GCGGCCGCGG ATCCACCATG GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG   181 AGTTCATGCG CTTCAAGGTG CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG   241 AGGGCGAGGG CGAGGGCCGC CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA   301 AGGGTGGCCC CCTGCCCTTC GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA   361 AGGCCTACGT GAAGCACCCC GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG   421 GCTTCAAGTG GGAGCGCGTG ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG   481 ACTCCTCCCT GCAGGACGGC GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC   541 CCTCCGACGG CCCCGTAATG CAGAAGAAGA CCATGGGCTG GGANGCCTCC TCCGAGCGGA   601 TGTACCCCGA GGACGGCGCC CTGAANGGCG AGNTCAAGCA GANGCTGAAG CTGNNGACGG   661 CGGCCACTAC GACGCTGANG NCNAGANNNC CTACNNNNCC NAGANNCCNN TGNAGCTGCC 721 NNGNGCCNNN NNNN //
Primer: PRE_rev (Pr0250) Region: PRE,insert 3'(hGem(1_110), AmCyan1 3') Sequence file: RDB15444_B1K5b.seq
>15444_15444_B1K5_3_PRE_rev_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNCANCATA GTTAAGAATA CCAGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGATTAC   121 AGCGCCTTTC TCCGTTTTTC TGCCACTTCC TTCCAATACT GAGAGGATGG ATTTTCTTTA   181 ATCATAAGAT CAAATGACTC CTGGGTGACT CCTCCAAGAT TTTTATTTTC ACTAGATTCT   241 GGGACAATAA CCCCAGGGCT GGAAGTTGTA GATGTTAAGT GGTCATTCCG ATGTTTCCTT   301 TTGGACAAGC CTGCGGACAG CTCATTTTCT CTTCCAACAA GAGATCCAGA TGCAGAAGGC   361 TGAATCATCT TCAGAGTTCT TCTTGGGACA GAACTATTCT TTATATTCTC TTTGATTTCT   421 TCTTGTTTCT GCTTCATACT GGGATTCATC TCGAGCGGCC GCCAGTGTGA TGGATATCCG   481 AAGGGCACCA CGGAGGTGAT GTGGGCCACG GCGTGCTCGG TCAGCTGCAC GCTGTTGCCG   541 CCCTTGTCCA GGTCGGTTCT GGCGATGCGG TGCTCCACCA CGTGGTTGGG GGGCATGGTC   601 ACGGGCTTCT TGGTCTTGTA NGANGTGTGG AACTGGCATC TGTAGTTGCC GCCGCCCTGC   661 AGCATCANGA ANGNGGNCNC GTCGCCCNTC NNNANGNCGT CNCANNCNGT CATCNNCTCN 721 ANNNNNGGGN NCNNNNNN //
Primer: mCherry-C_F (Pr0453) Region: insert mid(mCherry 3', hCdt1(30_120), P2A, AmCyan1 5') Sequence file: RDB15444_B1K5c.seq
>15444_15444_B1K5_3_mCherry-C_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN CCNNCCCCNG NCAGGCCCGC ANTCCGCGCC CCGGCCTCCG    61 CTAACCAGTG GCAGCCGCAA GCGCGCCCGC CCGCCCGCCG CCCCCGGACG CGACCAGGCC   121 AGGCCACCGG CCCGCAGGAG ACTGCGGCTG TCNGTGGACN AGGTTTCCAG CCCCAGTACC   181 CCCGAGGCCC CAGACATCCC AGCCTGCCCT TCTCCGGGCC AGAAGATAAA GAAATCCACC   241 CCGGCAGCAG GTCAGCCGCC CCACCTGACA TCCGCGCAGG ACCAGGACAC CATCACCGGT   301 CGCGCCAAGC GCGGCAGCGG CGCCACCAAC TTCAGCCTGC TGAAGCAGGC CGGCGACGTG   361 GAGGAGAACC CCGGCCCCCG CGAATTCGCC ACCATGGCCC TGTCCAACNN NTTCATCGGC   421 GACGACATGA AGATGACCTA CCACATGNNN NGCTGCGTGA ACGGCCACTA CTTCACCGTG   481 AAGGGCGAGG GCAGCGGCAA GCCCTACGAG GGCACCCANA CCTCCACCTT CAAGGTGACC   541 ATGGCCAACG GCGGCCCCCT GGCCTTCTCC TTCGACATCC TGTCCACCGT GTTCATGTAC   601 NGCAACCGCT GCTTCACCGN CTACNCCNNN CAGCATGNCC GACNACTTCA AGCANGCCTT   661 CCCCGACNGN ATGTCCTACG AGNNANNCNT TNACCTACNA GGANNGNNGG CGNNNNCANN   721 GNCAGNNNGN NNATCAGCCT GNNNNNNNNN GCNNNNNGCA NNNGTCNCCT TCNNNNNCGN 781 GNNCTTCCCN NNCGANGNNN NNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5'LTR,HIV-1 psi Sequence file: RDB15444_B1K5d.seq
>15444_15444_B1K5_3_CMV-Forward_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNTTGNN NCNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC   121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA   181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG   241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC   301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG   361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG   421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT   481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC   541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC   601 TGGGACAGCT ACNACCNTCC CTTCNGACAG GNTCAGAAGA ACTTAGATCA TTATATAATA   661 CAGTAGCNAC CCTCNANNGT GTGCATCAAN NNANAGAGAN AAANNNNNNN NANGNANNCN 721 TNNNNNNNNN N //
Primer: HIVgp_R1 (Pr0132) Region: HIV-1 psi Sequence file: RDB15444_B1K5e.seq
>15444_15444_B1K5_3_HIVgp_R1_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNCTCTT TTTTCTCTCT GCACCACTCT TCTCTTTGCC TTGGTGGGTG    61 CTACTCCTAA TGGTTCAATT TTTACTACTT TATATTTATA TAATTCACTT CTCCAATTGT   121 CCCTCATATC TCCTCCTCCA GGTCTGAAGA TCAGCGGCCC TTGCTGTGCG GTGGTCTTAC   181 TTTTGTTTTG CTCTTCCTCT ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC   241 TTTGATGCAC ACAATAGAGG GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC   301 CTGTCTGAAG GGATGGTTGT AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA   361 ACAGGCCAGG ATTAACTGCG AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA   421 ATTTATATTT TTTCTTTCCC CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC   481 TCCCCCGCTT AATACTGACG CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA   541 AAATTTTTGG CGTACTCACC AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCAGCAA   601 GCCGAGTCCT GCGTCGAGAG AGCTCCTCTG GTTTCCCTTT CGCTTTCNAG TCCCTGTTCG   661 GGCGCCACTG CTAGAGANTT TCCNCACTGN CNNAANNNNN CTNANGGNNC TCTNGTNNNN 721 NGANTNNNNN NNNNN //
Primer: BGH_rev3 (Pr0885) Region: bGH pA,3'LTR Sequence file: RDB15444_B1K5f.seq
>15444_15444_B1K5_3_bGH_rev3_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNGGNNGN NGGGCANACA ACAGATGGCT GGCAACTAGA AGGCACAGTC    61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG   121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC   181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA   241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT   301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC   361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC   421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT   481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT   541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCN GTCCCCCCTT   601 TTCTTTTAAA AAGTGNCTAA NANCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGNAN   661 CTGANGTGNG ANNGGAAAAN CNNNCTNCTN CTNCTCNTGN GCTNCTANCN NGGNNCNNTC 721 NGNANNNNNN NNNN //
Primer: SV40pro_F (Pr0110) Region: EM7 pro,Zeo Sequence file: RDB15444_B1K5g.seq
>15444_15444_B1K5_3_SV40pro_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAN NNNTGNNGNN GCTTTTTTGG ANGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG NNCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGNTGGGCTT   601 CGGAATCGTT TTCCGGGANG CCGGCTGGAT GANCCTCCNG CGCGGGGNNC TCNTGCTGGA   661 NTTCTTCGCC CACCCCNNCT TGTTTATTGC AGCTTANANN GGTNACNAAT AAAGCNNNNN 721 NNTCNCNANN TNNNNNNAAN NNANNNNNTT TTTTTNNNN //
Primer: mRFP1-inner1 (Pr0443) Region: insert mid(mCherry 3', hCdt1(30_120) 5') Sequence file: RDB15444_B1K5h.seq
>15444_15444_B1K5_3_mRFP1-inner1_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNTCCTCCCT GNNNGGNNGN NCGNNTTCAT    61 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCNAN NGNCCCGTAA TGCANAAGAA   121 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG   181 CGAGATCAAG CAGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCTG AGGTCAAGAC   241 CACCTACAAG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT   301 GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAAC GCGCCGAGGG   361 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGGGA TATCCATCAC ACTGGCGGCC   421 GCTCGAGCCC AGCCCCGCCA GGCCCGCACT CCGCGCCCCG GCCTCCGCTA CCAGTGGNAN   481 CCGCAAGCGC GCCCGCCCGC CCGCCGCCCC CGGACGCGAC CNNNNNNNGC CACNGGNNCN   541 NNNGAGACTG CGGCTGTCNN NGGNNNAGGT TTCCAGCCCC NGTACCCCCG ANGCCCCNGA   601 NATNNNNNNN CTGNNNCNTC TCCNGNNCNN AAANATANNN NNNNNCNNCC NNNGGNNNCN   661 NGTCNNNCCG NCCCNNNNTG ANATCCGCGN AGGNACNNNN AANACCNNCN CNNNGNNNNN 721 GCCNNNNNNN CNNNNNNNNG NNNNCNNNNN NNNCTNNNNN NNNNN //
Primer: AmCyan1-H_F (Pr1071) Region: insert mid(AmCyan1 3',hGem(1_110) 5') Sequence file: RDB15444_B1K5i.seq
>15444_15444_B1K5_3_AmCyan1-H_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGCNNCCNG NNCTCCNCCT TCNAGGTGAC CATGGCCAAC    61 GGCGGCCCCC TGGCCTTCTC CTTCGACATC CTGTCCACCG TGTTCATGTA CGGCAACCGC   121 TGCTTCACCG CCTACCCCAC CAGCATGCCC GACTACTTCA AGCAGGCCTT CCCCGACGGC   181 ATGTCCTACG AGAGAACCTT CACCTACGAG GACGGCGGCG TGGCCACCGC CAGCTGGGAG   241 ATCAGCCTGA AGGGCAACTG CTTCGAGCAC AAGTCCACCT TCCACGGCGT GAACTTCCCC   301 GCCGACGGCC CCGTGATGGC CAAGAAGACC ACCGGCTGGG ACCCCTCCTT CGAGAAGATG   361 ACCGTGTGCG ACGGCATCTT GAAGGGCGAC GTGACCGCCT TCCTGATGCT GCAGGGCGGC   421 GGCAACTACA GATGCCAGTT CCACACCTCC TACAAGACCA AGAAGCCCGT GACCATGCCC   481 CCCAACCACG TGGTGGAGCA CCGCATCGCC AGAACCGACC TGGACAAGGG CGGCAACAGC   541 GTGCAGCTGA CCGAGCACGC CGTGGCCCAC ATCACCTCCG TGGTGCCCTT CGGATATCCA   601 TCACACTGGC GGCCGCTCGA GANGAATCCC NGTATGAAGC ANNNNNAGAA NAANNCAAAN   661 AGAATANAAA GAATANTTCT GTCCNNNNAA NANNTCNNNN NNNGANTCNN CCNTCNNNNN 721 CNNNNNNNNN N //
Primer: EF1a-N_R (Pr0024) Region: EF1alpha pro,cPPT_CTS,RRE Sequence file: RDB15444_B1K5j.seq
>15444_15444_B1K5_3_EF1a-N_R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTGC GCTCTGCCNN CTGACGGGCA CCGGNNCCTC    61 ACGAAGCTTG AATTAATTCA AAGTGAATTT CTGCTGTCCC TGTAATAAAC CCGAAAATTT   121 TGAATTTTTG TAATTTGTTT TTGTAATTCT TTAGTTTGTA TGTCTGTTGC TATTATGTCT   181 ACTATTCTTT CCCCTGCACT GTACCCCCCA ATCCCCCCTT TTCTTTTAAA ATTGTGGATG   241 AATACTGCCA TTTGTAGATC CGTTCACTAA TCGAATGGAT CTGTCTCTGT CTCTCTCTCC   301 ACCTTCTTCT TCTATTCCTT CGGGCCTGTC GGGTCCCCTC GGGGTTGGGA GGTGGGTCTG   361 AAACGATAAT GGTGAATATC CCTGCCTAAC TCTATTCACT ATAGAAAGTA CAGCAAAAAC   421 TATTCTTAAA CCTACCAAGC CTCCTACTAT CATTATGAAT AATTTTATAT ACCACAGCCA   481 ATTTGTTATG TTAAACCAAT TCCACAAACT TGCCCATTTA TCTAATTCCA ATAATTCTTG   541 TTCATTCTTT TCTTGCTGGT TTTGCGATTC TTCAATTAAG GAGTGTATTA AGCTTGTGTA   601 ATTGTTAATT TCTCTGTCCC ACTCCATCCA GGTCGTGTGA TTCCAAATCT GTTCCAGAGA   661 TTTATTACTC CAACTAGCAT TCCAAGGCAC AGCAGTGGTG CAAATGAGTT TTCCAGAGCA   721 ACCCCAAATC CCCAGGAGCT GTTGATCCTT TANGTATCTT TCCACAGCCA GGATTCTTGC   781 CTGGAGCTGC TTGATGCCCC AGACTGTGAG TTGCAACAGA TGCTGTTGCG CCTCAATANC   841 CCTCAGCAAA TTGTTCTGCT GCTGCACTAT ACCNNANAAT AATTGTCTGG NNNGTNCCGT   901 CNNNCNNCAN NGACNCNNCG CCNNTANNGC TTCCNGCTGC TCCCNANANC CNNNNGNANN 961 AAAGCNCCNA TTNCNNNNNN NNNN //
Primer: Cdt1_F1 (Pr0768) Region: insert mid(P2A, AmCyan1 5') Sequence file: RDB15444_B1K5k.seq
>15444_15444_B1K5_Cdt1_F1_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNG NNNCCNTCAC CGGTCGCGCC NAGCGCGGCA    61 GCGGCGCCAC CAACTTCAGC CTGCTGAAGC AGGCCGGCGA CGTGGAGGAG AACCCCGGCC   121 CCCGCGAATT CGCCACCATG GCCCTGTCCA ACAAGTTCAT CGGCGACGAC ATGAAGATGA   181 CCTACCACAT GGACGGCTGC GTGAACGGCC ACTACTTCAC CGTGAAGGGC GAGGGCAGCG   241 GCAAGCCCTA CGAGGGCACC CAGACCTCCA CCTTCAAGGT GACCATGGCC AACGGCGGCC   301 CCCTGGCCTT CTCCTTCGAC ATCCTGTCCA CCGTGTTCAT GTACGGCAAC CGCTGCTTCA   361 CCGCCTACCC CACCAGCATG CCCGACTACT TCAAGCAGGC CTTCCCCGAC GGCATGTCCT   421 ACGAGAGAAC CTTCACCTAC GAGGACGGCG GCGTGGCCAC CGCCAGCTGG GAGATCAGCC   481 TGAAGGGCAA CTGCTTCGAG CACAAGTCCA CCTTCCACGG CGTGAACTTC CCCGCCGACG   541 GCCCCGTGAT GGCCAAGAAG ACCACCGGCT GGGACCCCTC CTTCGAGAAG ATGACCGTGT   601 GCGACGGCAT CTTGAAGGGC GACGTGACCG CCTTCCTGAT GCTGCAGGGC GGCGGCAACT   661 ACAGATGCCA GTTCCACACC TCCTACAAGA CCAAGAAGCC CGTGACCATG CCCCCCAACC   721 ACGTGGTGGA GCACCGCATC GCCAGAACCG ACCTGGACAA GGGCGGCAAC AGCGTGCAGC   781 TGANCGANCA CGCCGTGNCC CACATCACCT CCGNGGTGNN CTTCNGANAT CCATCACACT   841 GGCGGCCGCT CNANATGAAT CCCAGTATGA AGCANAAACN AGAAGAAATC NAAGANAATA   901 TNAAGAANAG TTCTGTCCNN ANAAGAANTC TNNNNNNNNN TCANCNNNCT GCNNCNNNNT 961 NNNNNNNNNG NNGANAAAAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.12.12

GNP_filter3_RDBDEP_html_231212.pl