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pCAGGS-BLRP-BirA

Expression vector to perform ChIP-seq analysis using biotinylated proteins.

Catalog number RDB15774
Resource name pCAGGS-BLRP-BirA
Clone info. Insertion of an ORF to this plasmids vector results in expression of a biotinylated proteins in animal cells, which is suitable for high-sensitivity ChIP-seq analyses. SmaI site is available to make target protein plus C-terminal fusion of biotin ligase recognition peptide (BLRP). Please refer Fig.1A and Fig.S5 of Matsuda, K. et al., Development 144 (11): 1948-1958 (2017) for the constitution and nucleotide sequence of pCAGGS-BLRP-BirA, respectively.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pCAGGS (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name E. coli biotin ligase genomic DNA
Depositor|Developer Kondoh, Hisato | Boss, Jeremy M. |
Other clones in our bank
External Database
E. coli biotin ligase
          Reference sequence
            
           
          Sequence (full) RDB15774zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Matsuda, K. et al., Development 144 (11): 1948-1958 (2017)).
          Additional terms and conditions:
          Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Matsuda, K. et al., Development 144 (11): 1948-1958 (2017))。
          付加的提供条件：
          CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
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          提供依頼書 [Word]
          提供同意書 (MTA, 非営利学術目的用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15774 pCAGGS-BLRP-BirA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCAGGS-BLRP-BirA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15774).

          Reference section:

          Matsuda, K., Mikami, T., Oki, S., Iida, H., Andrabi, M., Boss, J.M., Yamaguchi, K., Shigenobu, S., Kondoh, H., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958 (2017). PMID 28455373. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag (English text)
          Featured content Stable ChIP-seq analysis using transcription factors tagged with biotinylated peptide tag (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15774_A8Avp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V2 (Pr0330)
          Region: CAG pro,BLRP,6XHis,IRES
          Sequence file: RDB15774_A8Ava.seq check
          >15774_15774_A8Av_pCAG-F_V2_H02_23_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNG CGGNNNCNAN TCTGGGNNGC GCCGCCGCAC CCCNTCTAGC
             61 GGGCGCGGGG CGAAGCGGTG CGGCGCCGGC AGGAAGGAAA TGGGCGGGGA GGGCCTTCGT
            121 GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC
            181 GGCTGCCTTC GGGGGGGACG GGGCAGGGCG GGGTTCGGCT TCTGGCGTGT GACCGGCGGC
            241 TCTAGAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC
            301 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TGCACCCCCG GGATGGCTGG
            361 TGGCCTCAAC GACATCTTCG AGGCCCAGAA GATCGAGTGG CATGAAGATA CTGGTGGATC
            421 AGAGAATTTG TATTTTCAGT CTCATCATCA CCATCACCAT GCTAGCGGCC GCTAAGCAGA
            481 TCTGCGGCCG CTCGAGGAAT TCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG
            541 CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT
            601 TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT
            661 TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT
            721 GGAAGCTTCT TGAAGANAAA CAACGTCTGT AGCGANNNTT TGCAGGCAGC GGANNNNNNN
            781 NACCTGNCGA CAGGTGNNTC TGCGGNCAAA AGCCACGTGT ATAANATACA CCTGNAAANG
            841 CGGCACAACC NCNGNGCCNC GTTGTGAGTT GGATANTTGT GNAAAGANNN AAATGGNNNN
          901 NNNNNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES,BirA 5'
          Sequence file: RDB15774_A8Avb.seq check
          >15774_15774_A8Av_IRES-F_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCGANGTNNN NNNACGTCTA GGCCCCCCNA ACCACGGGGN
             61 NGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC ACTCGAGGCC GGCAAGGCCG
            121 GATCCACCAT GAAGGATAAC ACCGTGCCAC TGAAATTGAT TGCCCTGTTA GCGAACGGTG
            181 AATTTCACTC TGGCGAGCAG TTGGGTGAAA CGCTGGGAAT GAGCCGGGCG GCTATTAATA
            241 AACACATTCA GACACTGCGT GACTGGGGCG TTGATGTCTT TACCGTTCCG GGTAAAGGAT
            301 ACAGCCTGCC TGAGCCTATC CAGTTACTTA ATGCTAAACA GATATTGGGT CAGCTGGATG
            361 GCGGTAGTGT AGCCGTGCTG CCAGTGATTG ACTCCACGAA TCAGTACCTT CTTGATCGTA
            421 TCGGAGAGCT TAAATCGGGC GATGCTTGCA TTGCAGAATA CCAGCAGGCT GGCCGTGGTC
            481 GCCGGGGTCG GAAATGGTTT TCGCCTTTTG GCGCAAACTT ATATTTGTCG ATGTTCTGGC
            541 GTCTGGAACA AGGCCCGGCG GCGGCGATTG GTTTAAGTCT GGTTATCGGT ATCGTGATGG
            601 CGGAAGTATT ACGCAAGCTG GGTGCAGATA AAGTTCGTGT TAAATGGCCT AATGACCTCT
            661 ATCTGCAGGA TCGCAAGCTG GCAGGCATTC TGGTGGAGCT GACTGGCAAA ACTGGCGATG
            721 CGGCGCNNNT AGTCATTGGA GCCGGGATCA ACATGGCAAT GCGCCGTGTT GAAGAGAGTG
            781 TCGTTAATCA NNNGTGGATC ACGCTGCANG ANNCGGGGNT CAATCTCGAT CGTANTACGT
            841 TGGCGGCCAT GCTAATACGT GAATTACGNG CTGCNTNGGA ACTCTTCNAN CAANAANNNN
            901 NNNNNCNNTA NCTGNNNCGC NNGNNAANGC NGGANANTTT TATTNNNCNC CNNNNNNANN
          961 NNNNNNNN
          //
          Primer: pCA-bgter (Pr0066)
          Region: BirA 3'
          Sequence file: RDB15774_A8Avc.seq check
          >15774_15774_A8Av_pCA-bgter_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN TTTGGNNNNN GNNNNNNNNC TCAGNGGTAT TTGTGAGCCA
             61 GGGCATTGGC CACACCAGCC ACCACCTTCT GATAGGCAGC CTGCACCTGA GGAGTGAATT
            121 TTATTTTTCT GCACTACGCA GGGATATTTC ACCGCCCATC CAGGGTTTTA TTATTCCATC
            181 CTGCTCAAGT AATAAAGCCC CCTGTTTGTC TATTCCGCGT GAAATGCCAA ATATTTCTTT
            241 ATCACCAATG ATAAGTTTCA CTGGGCGATT AATAAAATTA TCCAGCTTTT CCCAGCGCGA
            301 CAGATAAGGT GCCAATCCTT CTTGTTCGAA GAGTTCCAAC GCAGCACGTA ATTCACGTAT
            361 TAGCATGGCC GCCAACGTAT TACGATCGAG ATTGATCCCC GCTTCCTGCA GCGTGATCCA
            421 CCCCTGATTA ACGACACTCT CTTCAACACG GCGCATTGCC ATGTTGATCC CGGCTCCAAT
            481 GACTATTTGC GCCGCATCGC CAGTTTTGCC AGTCAGCTCC ACCAGAATGC CTGCCAGCTT
            541 GCGATCCTGC AGATAGAGGT CATTAGGCCA TTTAACACGA ACTTTATCTG CACCCAGCTT
            601 GCGTAATACT TCCGCCATCA CGATACCGAT AACCAGACTT AAACCAATCG CCGCCGCCGG
            661 GCCTTGTTCC AGACGCCAGA ACATCGACAA ATATAAGTTT GCGCCAAAAG GCGAAAACCA
            721 TTTCCGANCC CGGCGACCAC GGCCAGCCTG CTGGTATTCT GCAATGCAAG CATCGCCCGA
            781 NTTAAGCTCT CCGATACGAT CAAGAANGNA CTGATTCGTG NANTCNATCA CNGGNAGCAC
            841 GGCTACNNNA CCGCCNTCCN GCNGANCCNA TATCTGTTNA NNANNNANTA ANNGNATANG
            901 NNCNNGNNNN CNGTATNNTT TANNNNGNNN NGNAAANNNA TCNNNNCCCC NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Matsuda, K., ChIP-seq analysis of genomic binding regions of five major transcription factors highlights a central role for ZIC2 in the mouse epiblast stem cell gene regulatory network. Development 144 (11): 1948-1958 (2017). PMID 28455373. [link to RRC of NBRP]
          user_report Komai, M., Nuclear SphK2/S1P signaling is a key regulator of ApoE production and Aβ uptake in astrocytes. J. Lipid Res. 65 (3): 100510 (2024). PMID 38280459. [link to RRC of NBRP]

          2024.04.04

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