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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Achilles/pRSETB

Yellow fluorescent protein clone Achilles.

Catalog number RDB15982
Resource name Achilles/pRSETB
Clone info. Yellow fluorescent protein clone Achilles. Regarding the comparative characterization of Achilles versus Venus, please refer Extended Data Fig.1 of Yoshioka-Kobayashi, K. et al., Nature (2020). https://doi.org/10.1038/s41586-019-1882-z.
Vector backbone pRSET B (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Miyawaki, Atsushi | Niino, Yusuke |
Other clones in our bank

External Database
Aequorea victoria GFP

            Reference sequence
              
             
            Sequence (full) RDB15982zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
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            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 1. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
            2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
            3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
            Yoshioka-Kobayashi, K. et al. (2020) Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123.
            4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE.
            5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR.
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考 提供条件は英文のまま記載してください。
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB15982 Achilles/pRSETB DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The Achilles/pRSETB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15982).

            Reference section:

            Yoshioka-Kobayashi, K., Matsumiya, M., Niino, Y., Isomura, A., Kori, H., Miyawaki, A., Kageyama, R., Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123 (2020). PMID 31915376. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Achilles, a novel fluorescent protein. (English text)
            Featured content Achilles, a novel fluorescent protein. (Japanese text)
            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15982_A8Fip1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: T7_20bp (Pr0261)
            Region: 6xHis, T7 tag, Xpress tag, insert 5'
            Sequence file: RDB15982_A8Fic.seq check
            >D05723A1_A8Fi_2_T7 20bp_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN TTGNTNACTT TAAGAAGGAG NNATACATAT GCGGGGTTCT
               61 CATCATCATC ATCATCATGG TATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGGGAT
              121 CTGTACGACG ATGACGATAA GGATCCGATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG
              181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGCGC
              241 GGCGAGGGCG AGGGCGATGC CACCATCGGC AAGCTGACCC TGAAGCTGAT CTGCACCACC
              301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG CCTGGCCGTC
              361 TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA
              421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC
              481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CTCCGGCTTC
              541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTTCAACAG CCACAACGTC
              601 TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT CCGCCACAAC
              661 ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC
              721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGANCTACC AGTCCTTCCT GAGCAAAGAN
              781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CNGGATCACT
              841 CTCGGCATGG NNGANCTGTA CNAGTAAGAN NTCNAAGCTN GATNCGGCNG CTAACAAANC
              901 CCGAAAGGAA GCTNANTTNG CTGCNGCCAC NGCNNNNCNN TNNNNANNAT NNNCCCNTNN
            961 GGGGNCNTCT NANNGNNNNN NNNGGNNTTT TTNNNNNNAN NNNNNNNNN
            //
            Primer: T7 terminator (Pr0155)
            Region: insert 3'
            Sequence file: RDB15982_A8Fid.seq check
            >D05723A1_A8Fi_2_T7 Terminator_G01_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CCGGNNNNNN CTTCGAATTC TTACTTGTAC
               61 AGCTCGTCCA TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA
              121 TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG AAGGACTGGT AGCTCAGGTA GTGGTTGTCG
              181 GGCAGCAGCA CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC
              241 ACGCCGCCGT CCTCGATGTT GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC
              301 TTGTCGGCGG TGATATAGAC GTTGTGGCTG TTGAAGTTGT ACTCCAGCTT GTGCCCCAGG
              361 ATGTTGCCGT CCTCCTTGAA GCCGGAGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG
              421 CCCTCGAACT TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG
              481 CGCTCCTGGA CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG
              541 TCGGGGTAGC GGGCGAAGAC GGCCAGGCCG TAGCCCAGGG TGGTCACGAG GGTGGGCCAG
              601 GGCACGGGCA GCTTGCCGGT GGTGCAGATC AGCTTCAGGG TCAGCTTGCC GATGGTGGCA
              661 TCGCCCTCGC CCTCGCCGCG CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG
              721 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCANCAT CGGATCCTTA
              781 TCGTCATCGT CGTACAGATC CCGACCCATT TGCTGTCCAC CAGTCATGCT ANCCATACCA
              841 NGATGANGAT GATGATGANA ACCCCGCATA TGTATATCTN CTTCTTAAAG TTAAACAAAA
            901 TTATTTCTAG NNGGGAAACC GTTGTGNTCT NCCTATAGTG AGTNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Yoshioka-Kobayashi, K., Coupling delay controls synchronized oscillation in the segmentation clock. Nature 580 (7801): 119-123 (2020). PMID 31915376. [link to RRC of NBRP]
            reference Diaz-Cuadros, M., In vitro characterization of the human segmentation clock. Nature 580 (7801): 113-118 (2020). PMID 31915384.

            2024.03.26

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