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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Sec63N-V5-GFP11

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP11; location: endoplasmic reticulum.

Catalog number RDB16048
Resource name Sec63N-V5-GFP11
Alternative name pMM89, pcDNA3.1_Sec63N-V5-GFP11, pcDNA3.1_Sec63_GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human SEC63 homolog, protein translocation regulator (Sec63), N-terminal segments 1-240 fused with V5-GFP11 at C-terminus, CMV promoter, bGH terminator, poly (A) signal, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pcDNA3.1 (plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human SEC63 cDNA
Depositor|Developer Tamura, Yasushi |
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External Database
human SEC63

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
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          RDB16048 Sec63N-V5-GFP11 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The Sec63N-V5-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16048).

          Reference section:

          Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          Featured content

          Featured content Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells (English text)
          Featured content Fluorescent protein probe that can visualize the multiple inter-organelle contact sites in cells (Japanese text)
          Featured content Visualization of Organelles (English text)
          Featured content Visualizing multiple inter-organelle contact sites (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB16048_A8I3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,insert 5'(Sec63(1-240) 5')
          Sequence file: RDB16048_A8I3d.seq check
          >D05803_B3_A8I3_1_CMV-Forward_D09_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTGN NGCNNNGGGC GGTANGCGTG TACGGTGGGA NGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG CGTTTAAACT TAAGCTTGGT ACCGAGCTCG
            181 GATCCGCCAC CATGGCCGGA CAACAGTTCC AGTATGACGA CTCAGGGAAC ACCTTCTTCT
            241 ACTTCCTGAC ATCCTTCGTC GGCCTGATCG TCATACCAGC GACCTACTAC CTCTGGCCCA
            301 GAGATCAGAA CGCAGAACAG ATACGGCTCA AGAACATTCG TAAGGTGTAT GGACGGTGCA
            361 TGTGGTACCG CCTTAGACTG CTGAAACCTC AACCCAACAT CATACCCACT GTCAAGAAAA
            421 TCGTGCTGTT GGCAGGATGG GCTCTGTTTC TCTTTCTGGC CTACAAAGTG AGCAAGACAG
            481 ACAGGGAGTA TCAGGAGTAC AACCCGTATG AAGTGCTCAA TCTTGATCCA GGCGCTACAG
            541 TGGCCGAAAT CAAGAAGCAG TATCGATTGT TGAGCCTCAA GTATCACCCC GATAAAGGAG
            601 GCGATGAGGT GATGTTTATG CGCATTGCCA AAGCGTATGC AGCACTGACT GACGAAGAGT
            661 CTAGGAAGAA TTGGGANGAN TTTGGGAATC CTGATGGTCC ACAAGCTACC TCCTTTGGTA
            721 TCGCCCTTCC TGCTTGGATC GTTGACCAGA ANAACAGTAT TCTGGTCCTG CTGGTTTACN
            781 GACTTGCCTT TATGGTGATT CTGCCAGTAN NANTTGGGNC ATGGNGGNAT ANGNCCATCA
            841 GANACNGCGG GNNNNAGANT CNGNNNNNNN NGANNCANNT TTACNCCNAC TTCNNNNNNN
          901 AANNCNNNNN NNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'(GFP11,V5,Sec63(1-240) 3')
          Sequence file: RDB16048_A8I3e.seq check
          >D05803_B3_A8I3_1_BGH_rev2_E09_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNGGGGG NNGGNNNNNN ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGGGTTTA AACGGGCCCT CTAGATTATG TAATCCCAGC AGCATTTACG
            121 TACTCATGAA GGACCATGTG GTCACGACTT GTTGATCCGC CACCAGACCC TCCATCGGCG
            181 GCCGCGCCGC CAGAACCGCC AGTACTATCC AGCCCCAACA GGGGATTCGG GATAGGTTTG
            241 CCATTGCGGG TTTTGTACAC GAAGTAGGTG TAAATCTGTG TCGTCCGAAT CAGAATCTGG
            301 TCCCCGCTGT ATCTGATGGA CCTATACCAC CATGACCCAA CTACTACTGG CAGAATCACC
            361 ATAAAGGCAA GTCCGTAAAC CAGCAGGACC AGAATACTGT TCTTCTGGTC AACGATCCAA
            421 GCAGGAAGGG CGATACCAAA GGAGGTAGCT TGTGGACCAT CAGGATTCCC AAACTCCTCC
            481 CAATTCTTCC TAGACTCTTC GTCAGTCAGT GCTGCATACG CTTTGGCAAT GCGCATAAAC
            541 ATCACCTCAT CGCCTCCTTT ATCGGGGTGA TACTTGAGGC TCAACAATCG ATACTGCTTC
            601 TTGATTTCGG CCACTGTAGC GCCTGGATCA AGANTGAGCA CTTCATACGG GTTGTACTCC
            661 TGATACTCCC TGTCTGTCTT GCTCACTTTG TANGCCAGAA AGAGAAACAG AGCCCATCCT
            721 GCCAACAGCA CGATTTTCTT GACAGTGGGT ATGANGNTGG GGTTGANGNT TCAGCAGTCT
            781 AANGCGGTAC CNCATGCACN NTCCNTACNC CTNACGANNG NTCNNGANCN NTANCTGNNC
          841 TGCGTTCNGA TNNCNGGNNN NNANNNAGTN NNNCGCNNGN ATGNNNANNN NNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB16048_A8I3f.seq check
          >D05803_B3_A8I3_1_SV40pro_F_F09_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNTGNN GNNGCTTTTT TGGNNGCCTA GGCTTTTGCA
             61 AAAAGCTCCC GGGNGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
            121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
            181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
            241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
            301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
            361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
            421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
            481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
            541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
            601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
            661 TGCCCGACGG CGANGNTCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
            721 TGGAAAATGG CCGCTTTTCT GGANTCATCG ACTGTNGCCG GCTGGGTGTG GCGGANCGCT
            781 ATCANGNNAT AGCGTTGGCT ANNNGTGATA TTGCTGAAGA NCTTGGCGGC GAANGGGCTG
            841 NNCGCNTCCN CGNGCTTTAC NGNANCGCNN NNNCNNNNNC NNANCGCATC GNNTNNTANC
          901 NNCTNNNNNN NGANTNCTTN NNANCNGNNN NNNNGGGNNN NNNANNNNNN NNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

          2024.03.26

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