Please review the QC test results indicated by icon below as well as clone information before placing your order.

Dpp1-V5-GFP11

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: vacuole.

Sequence (full)	RDB16050zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16050_A8I3p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800) Region: T7 pro,insert 5'(GPD pro) Sequence file: RDB16050_A8I3g.seq
>16050_16050_B2ApF_1_HB-NOS_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNGGNNNN NNCCAGGGTT TTCCCNGTCA CGACGTTGTA    61 AAACGACGGC CAGTGAATTG TAATACGACT CACTATAGGG CGAATTGGAG CTCAGTTTAT   121 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA   181 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT   241 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT   301 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT   361 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC   421 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA   481 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC   541 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGANT TGGAAAAAGC TGAAAAAAAA   601 GGNTGAAACC AGTTCCCTGA AATTATTCCC CTACTTGANT AATAAGTATA TAAAGACGGT 661 ANGNANTGAN TGNNATTCNG NAAATCNANT TCNNANNNNN NNNNN //
Primer: pUC-CAPbs_up_F (Pr0536) Region: lac pro,lacO,T3 pro,insert 3'(CYC1 ter, GFP11,V5 tag) Sequence file: RDB16050_A8I3h.seq
>16050_16050_B2ApF_1_pUC-CAPbs_up_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNNN NCAATTAATG TGAGTTAGNN NNNNCATTAG    61 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA   121 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG CCAAGCTCGA AATTAACCCT   181 CACTAAAGGG AACAAAAGCT GGTACCGGCC GCAAATTAAA GCCTTCGAGC GTCCCAAAAC   241 CTTCTCAAGC AAGGTTTTCA GTATAATGTT ACATGCGTAC ACGCGTCTGT ACAGAAAAAA   301 AAGAAAAATT TGAAATATAA ATAACGTTCT TAATACTAAC ATAACTATAA AAAAATAAAT   361 AGGGACCTAG ACTTCAGGTT GTCTAACTCC TTCCTTTTCG GTTAGAGCGG ATGTGGGGGG   421 AGGGCGTGAA TGTAAGCGTG ACATAACTAA TTACATGACT CGAGGTCGAC GGTATCGATA   481 AGCTTGATAT CGAATTCTTA GGTAATGCCT GCAGCATTGA CATATTCGTG TAGAACCATA   541 TGATCTCTGC TTGTGGAACC ACCACCACTT CCACCAGTTG AGTCTAAGCC CAAAAGANGG   601 TTTGGTATGG GTTTCCCAGA NCCTCCGGCA GANGATCCNC TAGTCATACC TTCNTCNGAN 661 NAAGGANGTA NTTCCTCNTN NGGNNNNCTC TGANGGNNGA NNGNNNNN //
Primer: CYC1ter_R (Pr0022) Region: insert mid(CYC1 ter, GFP11,V5 tag,Dpp1 3') Sequence file: RDB16050_A8I3i.seq
>D05803_B5_A8I3_1_CYC1ter_R_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNC GGNNGTGGGG GGNNGGCGTG AATGTAAGCG TGNCATAACT    61 AATTACATGA CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCT TAGGTAATGC   121 CTGCAGCATT GACATATTCG TGTAGAACCA TATGATCTCT GCTTGTGGAA CCACCACCAC   181 TTCCACCAGT TGAGTCTAAG CCCAAAAGAG GGTTTGGTAT GGGTTTCCCA GAACCTCCGG   241 CAGAGGATCC ACTAGTCATA CCTTCATCGG ACAAAGGATG TAATTCCTCA TCCGGGATCC   301 TCTGATGGGT GACTGCTTCC TCCAGGGTGA CATCTGAATC GTCCATCAAT GGTTTGAACG   361 GAAGAGGATC ATCAATGGGT GGGAAGATTC TTCTGTAGAA AAAGTGTGCC ATTATATAAC   421 CCAACATAGA CCCTAAAATT ACATCGACGA AATGATGTCT GTAATCTTGA GTTCTGGATA   481 GAGCAATTAG TGCAGCTCCT AACAGTGGTA GAAAGGCCAC CATTTTTCTC CATAAAGGCA   541 TCAACGGTGA TTCAGTCAAA AGTTGCCCAC ATAGCCAGAA GTACAAATAA CCCAGTCCTG   601 CAAAGCTTTC ACTTGAATGA CCTGACGGAG TTGTCCTAAA CCCATCCAAC AGACGTTCGT   661 GATTCTTAGT CGTACACACA TCTTTTGCAG TAAATAAAGT GTCCAATGGC AAGCCTTCAA   721 CAGGTTGGCA ACGATCTAGA AAATCTGGTC TTAGTCTTCC AATCCAATTC TTGATGAAGT   781 TTGTAAAGAA ACTCGTACTG NACCAAGCGA GTGATAAACC AANGANANAT GTGTACAAAA   841 TAAAAAATCA AATGTCTTCT ATCGGCCNAA AATGNAACCA ATTATCAATA TGGTTAAANN   901 TGGNNCGACN AAANCTNTNA ANNAACNNCN TGNNGTTANT TNACNNNNNN NNNTNNNNNN 961 NNNANNNNNN NNNN //
Primer: TRP1pro_F (Pr0171) Region: TRP1 Sequence file: RDB16050_A8I3j.seq
>D05803_B5_A8I3_1_TRP1pro_F_G05_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNANG GCAGCTNGGN NTNNGTCTGT TATTAATTTC NCAGGTAGTT    61 CTGGTCCATT GNNNGAAAGT TTGCGGCTTG CAGAGCACAG AGGCCGCAGA ATGTGCTCTA   121 GATTCCGATG CTGACTTGCT GGGTATTATA TGTGTGCCCA ATAGAAAGAG AACAATTGAC   181 CCGGTTATTG CAAGGAAAAT TTCAAGTCTT GTAAAAGCAT ATAAAAATAG TTCAGGCACT   241 CCGAAATACT TGGTTGGCGT GTTTCGTAAT CAACCTAAGG AGGATGTTTT GGCTCTGGTC   301 AATGATTACG GCATTGATAT CGTCCAACTG CATGGAGATG AGTCGTGGCA AGAATACCAA   361 GAGTTCCTCG GTTTGCCAGT TATTAAAAGA CTCGTATTTC CAAAAGACTG CAACATACTA   421 CTCAGTGCAG CTTCACAGAA ACCTCATTCG TTTATTCCCT TGTTTGATTC AGAAGCAGGT   481 GGGACAGGTG AACTTTTGGA TTGGAACTCG ATTTCTGACT GGGTTGGAAG GCAAGAGAGC   541 CCCGAAAGCT TACATTTTAT GTTAGCTGGT GGACTGACGC CAGAAAATGT TGGTGATGCG   601 CTTAGATTAA ATGGCGTTAT TGGTGTTGAT GTAAGCGGAG GTGTGGAGAC AAATGGTGTA   661 AAAGACTCTA ACAAAATAGC AAATTTCGTC AAAAATGCTA AGAAATAGGT TATTACTGAG   721 TAGTATTTAT TTAAGTATTG TTTGTGCACT TGCCGATCTA TGCGGTGTGA AATACCGCAC   781 AGATGCGTAA NGAGAAAATA CCGCATCANG AAATTGTAAG CGTTAATATN NTGNTAAAAT   841 TCGCGTTAAA TTTTTNGNTA AATCAGCTCA TTTTTTTAAN NNATANGCNN AAATCGGCAA   901 ANTCCNNTAN AANNCAAAAG NNNAGANNNA NATAGGNNNN NNNGNNGTTN NNNNTNNGNN 961 NNNNNNNN //
Primer: M13_-40 (Pr0410) Region: insert mid(GDP pro,Dpp1 5') Sequence file: RDB16050_A8I3k.seq
>D05803_B5_A8I3_1_M13_-40_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NTNNCNATAG GGCGAANTGG AGCTCAGTTT ATCATTATCA    61 ATACTCGCCN NNNNNNNNAN NACGTAAATA ATTAATAGTA GTGATTTTCC TAACTTTATT   121 TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA CATGCCCAAA ATAGGGGGCG   181 GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA CAGTTTATTC CTGGCATCCA   241 CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA AAAAAAAAGA ATCCCAGCAC   301 CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC CATTCTCTTA GCGCAACTAC   361 AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC TCAATGGAGT GATGCAACCT   421 GCCTGGAGTA AATGATGACA CAAGGCAATT GACCCACGCA TGTATCTATC TCATTTTCTT   481 ACACCTTCTA TTACCTTCTG CTCTCTCTGA TTTGGAAAAA GCTGAAAAAA AAGGTTGAAA   541 CCAGTTCCCT GAAATTATTC CCCTACTTGA CTAATAAGTA TATAAAGACG GTAGGTATTG   601 ATTGTAATTC TGTAAATCTA TTTCTTAAAC TTCTTAAATT CTACTTTTAT AGTTAGTCTT   661 TTTTTTAGTT TTAAAACACC AGAACTTAGT TTCGACGGAT GCGGCCGCAT GAACAGAGTT   721 TCGTTTATTA AAACGCCTTT CAACATAGGG GCGAAATGGA GATTAGAAGA NGTCTTTTTG   781 CTCATTATCA TGATACTTCT TAACTACCCA GTGTATTACC AACAACCGTT CGAACGTCAG   841 TTTTACATTA ACGATCTCAC TATATCGCAT CCTTATGCGA CAACNGAACG TGTAAATAAC   901 AACANGTNGT TNGNTTATAG TTTNGTCGNN NCNTCTTTNA NCNNANNNNN NATNNNNNNC 961 ATTTNNNNNN ANNNNANNNN NNTNNNNTTT TNNNTTTNNN NNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,CEN_ARS Sequence file: RDB16050_A8I3l.seq
>D05803_B5_A8I3_1_Amp_R_H05_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG CANTNATNNG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT ANAANAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGGGTCCT TTTCATCACG TGCTATAAAA ATAATTATAA TTTAAATTTT TTAATATAAA   181 TATATAAATT AAAAATAGAA AGTAAAAAAA GAAATTAAAG AAAAAATAGT TTTTGTTTTC   241 CGAAGATGTA AAAGACTCTA GGGGGATCGC CAACAAATAC TACCTTTTAT CTTGCTCTTC   301 CTGCTCTCAG GTATTAATGC CGAATTGTTT CATCTTGTCT GTGTAGAAGA CCACACACGA   361 AAATCCTGTG ATTTTACATT TTACTTATCG TTAATCGAAT GTATATCTAT TTAATCTGCT   421 TTTCTTGTCT AATAAATATA TATGTAAAGT ACGCTTTTTG TTGAAATTTT TTAAACCTTT   481 GTTTATTTTT TTTTCTTCAT TCCGTAACTC TTCTACCTTC TTTATTTACT TTCTAAAATC   541 CAAATACAAA ACATAAAAAT AAATAAACAC AGAGTAAATT CCCAAATTAT TCCATCATTA   601 AAAGATACGA GGCGCGTGTA AGTTACAGGC AAGCGATCCG TCTAAGAAAC CATTATTATC   661 ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTCGC GCGTTTCGGT   721 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGANA CGGTCACAGC TTGTCTGTAA   781 GCGGATGCCG GGANCAGACA AGCCNGTCAN GGCGCGTCAG CGGGNGTTGG CGGGNGNCGG   841 GGCTGGCTTA NCTATGCGGC ATCAGANCAN ANTGTACTGA NANTGCNCCN TANATCACGA   901 NNTTACNATN TATNTNATAN AGGNNNNNTT TNNTANNNNN NCANCNNNNN ANNNGNNNAN 961 NTNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.06

GNP_filter3_RDBDEP_html_230405.pl