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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Erg6-FLAG-GFP11 (#RDB16054)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: 3xFLAG-GFP11; location: lipid droplets.

Alternative name pSFL67, pRS314-GPDp-Erg6-3xFLAG-GFP11
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae sterol 24-C-methyltransferase (Erg6) fused with 3xFLAG-GFP11 at C-terminus, GPD promoter, CYC1 terminator, please refer to Fig.1 and Table S1 of Kakimoto, Y. et al. Sci. Rep., 8 (1): 6175, 2018.
Comment Expression was confirmed by the depositor with fluorescence microscopy.
Vector backbone pRS314 (plasmid)
Selectable markers Amp^r (E. coli), TRP1
Gene/insert name S. cerevisiae ERG6 cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB16054zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sci. Rep. 8: 6175, 2018)
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必要書類
提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Sci. Rep. 8: 6175, 2018) 。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB16054 Erg6-FLAG-GFP11 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Erg6-FLAG-GFP11 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16054).

Reference section:

Kakimoto, Y., Tashiro, S., Kojima, R., Morozumi, Y., Endo, T., Tamura, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB16054_A8I3p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: HB-NOS_R (Pr0800)
Region: T7 pro, insert 5' (GPD pro)
Sequence file: RDB16054_A8I3e.seq check
>16054_16054_B0JcF_HB-NOS_R_E02_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN AANTTGGNNN NNNCCNGGGT TTTCCCAGTC ACGACGTTGT
   61 AAAACGACGG CCAGTGAATT GTAATACGAC TCACTATAGG GCGAATTGGA GCTCAGTTTA
  121 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT
  181 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA
  241 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC
  301 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA
  361 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG
  421 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG
  481 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT
  541 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAN TTGGAAAAAG CTGAAAAAAA
  601 AGGNTGAAAC CAGTTCCCTG AAATTATTCC CCTACTTGAC TAATAAGTAT ATAAAGANNG
  661 NANGNANTGA NTGNAATTCT GNNNNNNATT TCNNNNNTTC NTAAATTCNN NTTTNNNANN
721 NAGTCTTTTT TTTNNNNTTT NNAANNNNNN NNNN
//
Primer: SV40pA3'Element Reverse (Pr0108)
Region: T3 pro, insert 3' (CYC1 ter)
Sequence file: RDB16054_A8I3k.seq check
>D05803_B9_A8I3_1_SV40pA3'element Reverse_F07_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGNNANGCN NGCAACGCGG CCTTTTNACG GTTCCTGGCC
   61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
  121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
  181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CCAATACGCA AACCGCCTCT CCCCGCGCGT
  241 TGGCCGATTC ATTAATGCAG CTGGCACGAC AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG
  301 CGCAACGCAA TTAATGTGAG TTAGCTCACT CATTAGGCAC CCCAGGCTTT ACACTTTATG
  361 CTTCCGGCTC GTATGTTGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC
  421 TATGACCATG ATTACGCCAA GCTCGAAATT AACCCTCACT AAAGGGAACA AAAGCTGGTA
  481 CCGGCCGCAA ATTAAAGCCT TCGAGCGTCC CAAAACCTTC TCAAGCAAGG TTTTCAGTAT
  541 AATGTTACAT GCGTACACGC GTCTGTACAG AAAAAAAAGA AAAATTTGAA ATATAAATAA
  601 CGTTCTTAAT ACTAACATAA CTATAAAAAA ATAAATAGGG ACCTAGACTT CAGGTTGTCT
  661 AACTCCTTCC TTTTCGGTTA GAGCGGATGT GGGGGGANGG CGTGAATGTA AGCGTGACAT
  721 AACTAATTAC ATGACTCGAG GTCGACGGTA TCGATAAGCT TGATATCGAA TTCTTATGTT
  781 ATCCCTGCAG CATTGNCGTA TTCATGCAAA ACCATGTGNT CTCTGGAAGT TGAACCTCCA
  841 CCACTTCCGC CTTTANCGNC ATCATCCTTA TANNCAANNN CATGNNNCTT ATAATCGCNN
901 TCANGNNNTT NNTANNCACN NCCGGNNNNN NNNNN
//
Primer: CYC1ter_R (Pr0022)
Region: insert mid (CYC1 ter, GFP11, 3xFLAG, Erg6 3')
Sequence file: RDB16054_A8I3l.seq check
>D05803_B9_A8I3_1_CYC1ter_R_C12_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNCN GNNGTGGGGG GNNGGCGTGA ANGTAAGCGT GACATAACTA
   61 ATTACATGAC TCGAGGTCGA CGGTATCGAT AAGCTTGATA TCGAATTCTT ATGTTATCCC
  121 TGCAGCATTG ACGTATTCAT GCAAAACCAT GTGATCTCTG GAAGTTGAAC CTCCACCACT
  181 TCCGCCTTTA TCGTCATCAT CCTTATAGTC AATGTCATGA TCCTTATAAT CGCCATCATG
  241 GTCTTTGTAA TCACCACCGG CAGAGGATCC TTGAGTTGCT TCTTGGGAAG TTTGGGAGGG
  301 GGTTTCGGCG TTTTCTGGCT TCCTAGCGAC GAAAAGCATC ATTGGAGTGA ATAACTTGGA
  361 CTTACCACCG GCAACTAAAC CAACCGCAGC ATTTTCTAGA GCAGCAGTAA CTTCCTTGGA
  421 ACCTTCTGGG GCTAGACCTA ATTTTTCCAT TACAGTAACC ATTGCTGTAG TAAATTGTCT
  481 ACCCAAGTAA GAAGTTCTGA AAAATGTGGC CAAATTAGCT AAGTTTTGAA CGTACTTCCA
  541 CTCACCAGTT AATGGGTAAT ACCAAGGGAT TTCATCATCA TTGTCCGCCA GGTCTTCGCT
  601 AACGAGGACT TCGAAACCAC AGTTCTTCAA TGCTTTCCTA GCCACGTCGA CATGGAACAT
  661 CTTTGGGATA CCATCACCTA GTTCAATTTC ATAAGCGATC TTTCTATGTT CAGGATTGTT
  721 TTCGTCATAT TTATCAGTCA TTACCCATTC GTAAACAGCA AAGGTACCAC CCGGTTTCNA
  781 AACCTTGTAG ATTTCGCTGT ATACACCTTC TAATTTTGGA NCGTGNCATG TGGCCTCANT
  841 TGCATAAACT TTGTCGAAAG NGTTTTCTNC NAANTNNATT TTNCATGAAN TCACCNNTNN
901 NNAANNNCAN TTNNGNCANT CNAANNNNNN TTTTTNNNNN NNNNN
//
Primer: TRP1pro_F (Pr0171)
Region: TRP1
Sequence file: RDB16054_A8I3m.seq check
>D05803_B9_A8I3_1_TRP1prp_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNCTTNNNN NNTGTCTGTT ATTAATTTNN
   61 CNNGGTAGTT CTGGTCCATT GGTGAAAGTT TGCGGCTTGC AGAGCACAGA GGCCGCAGAA
  121 TGTGCTCTAG ATTCCGATGC TGACTTGCTG GGTATTATAT GTGTGCCCAA TAGAAAGAGA
  181 ACAATTGACC CGGTTATTGC AAGGAAAATT TCAAGTCTTG TAAAAGCATA TAAAAATAGT
  241 TCAGGCACTC CGAAATACTT GGTTGGCGTG TTTCGTAATC AACCTAAGGA GGATGTTTTG
  301 GCTCTGGTCA ATGATTACGG CATTGATATC GTCCAACTGC ATGGAGATGA GTCGTGGCAA
  361 GAATACCAAG AGTTCCTCGG TTTGCCAGTT ATTAAAAGAC TCGTATTTCC AAAAGACTGC
  421 AACATACTAC TCAGTGCAGC TTCACAGAAA CCTCATTCGT TTATTCCCTT GTTTGATTCA
  481 GAAGCAGGTG GGACAGGTGA ACTTTTGGAT TGGAACTCGA TTTCTGACTG GGTTGGAAGG
  541 CAAGAGAGCC CCGAAAGCTT ACATTTTATG TTAGCTGGTG GACTGACGCC AGAAAATGTT
  601 GGTGATGCGC TTAGATTAAA TGGCGTTATT GGTGNNNNNN TAAGCGGAGG TGTGGAGACA
  661 AATGGTGTAA AAGACTCTAA CAAAATAGCA AATTTCGTCA AAAATGCTAA GAAATAGGTT
  721 ATTACTGANN AGTATTTATT TANGTATTGT TTGTGCACTT GCCGATCTAT GCGGTGTGAA
  781 ATACCGCACA NATGCGTAAG GAGAAAAATA CCGCATCANG GAAATTGNAA GCGTTAATAT
  841 TTTGTNNANT TCGCGTTNAA NNTTTTGTTN AATCAGCTCA NTTTTTANCC NANANGCCNA
  901 AANNNGNNAA ANNCCTTATA AATCNAAANA NNAGANCGAN NNNNNGGTTG ANTGNTNNNN
  961 NCNNTTNGGA NNAGAGTNCN CTATNNANNN NNCNNGNANN NNNNNGTCNA ANGNCNAAAA
1021 ACCGTNNATC NNGGNNANNN NNNNNACNNN NNNNNNN
//
Primer: TDH3pro_F (Pr0431)
Region: insert mid (GPD pro, Erg6 5')
Sequence file: RDB16054_A8I3n.seq check
>16054_16054_B0JcF_TDH3pro_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNGNNTGNN NNNCTGTAAA TCTATTTCTT AAACTTCTTA AATTCTACTT
   61 TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC GGATGCGGCC
  121 GCATGAGTGA AACAGAATTG AGAAAAAGAC AGGCCCAATT CACTAGGGAG TTACATGGTG
  181 ATGATATTGG TAAAAAGACA GGTTTGAGTG CATTGATGTC GAAGAACAAC TCTGCCCAAA
  241 AGGAAGCCGT TCAGAAGTAC TTGAGAAATT GGGATGGTAG AACCGATAAA GATGCCGAAG
  301 AACGTCGTCT TGAGGATTAT AATGAAGCCA CACATTCCTA CTATAACGTC GTTACAGATT
  361 TCTATGAATA TGGTTGGGGT TCCTCTTTCC ATTTCAGCAG ATTTTATAAA GGTGAGAGTT
  421 TCGCTGCCTC GATAGCAAGA CATGAACATT ATTTAGCTTA CAAGGCTGGT ATTCAAAGAG
  481 GCGATTTAGT TCTCGACGTT GGTTGTGGTG TTGGGGGCCC AGCAAGAGAG ATTGCAAGAT
  541 TTACCGGTTG TAACGTCATC GGTCTAAACA ATAACGATTA CCAAATTGCC AAGGNAAAAT
  601 ATTACGCTAA AAAATACAAT TTGAGTGANC AAATGGACTT TGTAAAGGGT GANTTCATGA
  661 AAATGGNNTT CNAAGANNNN CTTTCNANNA NNTTTATGCN NTTGNNNNNC ATGNNNCGCT
721 NCNAANNNNN NANNNNGNNN NNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro, CEN_ARS
Sequence file: RDB16054_A8I3j.seq check
>D05803_B9_A8I3_1_Amp_R_H07_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCANTTAT NAGGGTTATT GTCTCNTGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGGGT CCTTTTCATC ACGTGCTATA AAAATAATTA TAATTTAAAT TTTTTAATAT
  181 AAATATATAA ATTAAAAATA GAAAGTAAAA AAAGAAATTA AAGAAAAAAT AGTTTTTGTT
  241 TTCCGAAGAT GTAAAAGACT CTAGGGGGAT CGCCAACAAA TACTACCTTT TATCTTGCTC
  301 TTCCTGCTCT CAGGTATTAA TGCCGAATTG TTTCATCTTG TCTGTGTAGA AGACCACACA
  361 CGAAAATCCT GTGATTTTAC ATTTTACTTA TCGTTAATCG AATGTATATC TATTTAATCT
  421 GCTTTTCTTG TCTAATAAAT ATATATGTAA AGTACGCTTT TTGTTGAAAT TTTTTAAACC
  481 TTTGTTTATT TTTTTTTCTT CATTCCGTAA CTCTTCTACC TTCTTTATTT ACTTTCTAAA
  541 ATCCAAATAC AAAACATAAA AATAAATAAA CACAGAGTAA ATTCCCAAAT TATTCCATCA
  601 TTAAAAGATA CGAGGCGCGT GTAAGTTACA GGCAAGCGAT CCGTCTAAGA AACCATTATT
  661 ATCATGACAT TAACCTATAA AAATAGGCGT ATCNCGANGC CCTTTCGTCT CGCGCGTTNN
  721 GGTGATGANG GTGAAANNCT CTGANACNTG CAGCTNCCNG ANANGGTCAC AGCTNGTCTG
  781 TAAGCGGANG CCGGGANCAN ANANNCCCGT CANGNNNCGT CANNNGGTGT TNNCGGGNNN
  841 NGGGGNNNGN CNNNNCTANN CNNNNTCNNA NCANANTNGN NCTGANNNNN NNACCNNNNN
901 NNNCGANNNN NCNNNNTNTN TNNNNNNNNG NNNNNNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kakimoto, Y., Visualizing multiple inter-organelle contact sites using the organelle-targeted split-GFP system. Sci. Rep. 8 (1): 6175 (2018). PMID 29670150. [link to RRC of NBRP]