Flp/In targeting construct of Fucci2a
Clone info. | Flp/In targeting construct to make a single insertion into Flp/In compatible cell lines of Fucci2a a multicistronic version of Fucci2 composed of mVenus-hGem(1/110) and mCherry-hCdt1(30/120) fused with t2a. |
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Comment | This clone was used in generating stable cell lines by the depositor. |
Vector backbone | pCDNA5/Frt (Plasmid) |
Size of vector backbone | 5.0 kb |
Selectable markers | Ampicillin (E. coli), Hygromycin (mammalian cells) |
Gene/insert name | Human CDT1 cDNA Human Gem cDNA Discosoma sp. RFP cDNA Aequorea victoria GFP cDNA |
Depositor|Developer | Jackson, Ian | Mort, Richard | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] |
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Terms and conditions for distribution | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, (2014)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. Additional terms and conditions: 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki. |
Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
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MTAに書く使用条件 | 1) The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers and the BIOLOGICAL RESOURCE may not be used on humans. 2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designated by the DEPOSITOR (Mort, R.L. et al., Cell Cycle 13 (17): 2681-2696, (2014)) is requested. 3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Richard Mort of Lancaster University is requested. 4) The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. 5) The BIOLOGICAL RESOURCE may not be used for commercial purposes by the RECIPIENT. 6) The RECIPIENT may not transfer the BIOLOGICAL RESOURCE to any third parties. 付加的使用条件: 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact: license-contact@innovation-riken.jp 2. The RECIPIENT's Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of Dr. Atsushi Miyawaki. |
備考 | 提供条件は英文のまま記載してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB16056 | pCDNA5-Frt-Fucci2a | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pCDNA5-Frt-Fucci2a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16056). |
Reference section:
Mort, R.L., Ford, M.J., Sakaue-Sawano, A., Lindstrom, N.O., Casadio, A., Douglas, A.T., Keighren, M.A., Hohenstein, P., Miyawaki, A., Jackson, I.J., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB16056_A9K8p1-1.pdf ![]() |
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Nucleotide sequence of a portion of this resource (if available).
Primer: CMV_Forward (Pr0016) Region: CMV pro, T7 pro, insert 5' (mCherry) Sequence file: RDB16056_A9K8a.seq ![]() |
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>D05769A1_A9K8_1_CMV_Forward_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCNAATGG GCGGTANGCG TGTACGGTGG GNGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGAATTCCTC GACACGCGTA TGGTGAGCAA 181 GGGCGAGGAG GATAACATGG CCATCATCAA GGAGTTCATG CGCTTCAAGG TGCACATGGA 241 GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA 301 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCGCCTGGGA 361 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT 421 CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT 481 CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT 541 CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA 601 GACCATGGGC TGGGAGGCCT CCTCCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG 661 CGAGATCAAG CAGAGGCTGA AGCTGAANGA CGGCGGCCAC TACGACGCTG ANGTCAAGAC 721 CACCTACAAN GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAACGTCA ACATCAAGTT 781 GGACATCACC TCCCACAACG AGGNNTACAC CATCNNNGAA CAGTACGAAC GCGCCGANGG 841 CCGCNACTCC ACCGGCGGCA TGNNCGANCT GTACAAGGGN TATNCNTCAC ACNNGCGGNC 901 GCNCNNNCCN AGCCCCCGCC NGGNCNGCNC TNCNNNNCCC GGNCNTNNGN NANNNNNNNN 961 NN // |
Primer: BGH_rev2 (Pr0606) Region: BGH p(A), insert 3' (Gem1_1-110, mVenus) Sequence file: RDB16056_A9K8b.seq ![]() |
>D05769A1_A9K8_1_BGH_rev2_B06_06_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GNNGNNGGGC AAACAACAGN TGGCTGGCAA CTAGAAGGCA 61 CAGTCGAGGC TGATCAGCGG GTTTAAACGG CCCGGCGCGC CACATGTGGT ACCTTACAGC 121 GCCTTTCTCC GTTTTTCTGC CACTTCCTTC CAATACTGAG AGGATGGATT TTCTTTAATC 181 ATAAGATCAA ATGACTCCTG GGTGACTCCT CCAAGATTTT TATTTTCACT AGATTCTGGG 241 ACAATAACCC CAGGGCTGGA AGTTGTAGAT GTTAAGTGGT CATTCCGATG TTTCCTTTTG 301 GACAAGCCTG CGGACAGCTC ATTTTCTCTT CCAACAAGAG ATCCAGATGC AGAAGGCTGA 361 ATCATCTTCA GAGTTCTTCT TGGGACAGAA CTATTCTTTA TATTCTCTTT GATTTCTTCT 421 TGTTTCTGCT TCATACTGGG ATTCATCTCG AGCGGCCGCC AGTGTGATGG ATATCCCTTG 481 TACAGCTCGT CCATGCCGAG AGTGATCCCG GCGGCGGTCA CGAACTCCAG CAGGACCATG 541 TGATCGCGCT TCTCGTTGGG GTCTTTGCTC AGCTTGGACT GGTAGCTCAG GTAGTGGTTG 601 TCGGGCAGCA GCACGGGGCC GTCGCCGATG GGGGTGTTCT GCTGGTAGTG GTCGGCGAGC 661 TGCACGCCGC CGTCCTCGAT GTTGTGGCGG ATCTTGAAGT TGGCCTTGAT GCCGTTCTTC 721 TGCTTGTCGG CGGTGATATA GACGTTGTGG CTGTTGTAGT TGTACTCCAG CTTGTGCCCC 781 AGGATGTTGC CGTCCTCCTT GAAGTCGATG CCCTTCAGCT CGATGCGGTT CACCAGGGNG 841 TCGCCCTCNA NCTTCACCTC NGCGCGGGNC TNNTANTTGC CGNCNNNCTT GAAGAANANG 901 GNGCGNNNCN GGACGTANCC TTCGGGNNTG GNNGNNTNNN NNNNNNCNNG CNNNTTNNNN 961 NNNNNN // |
Primer: HygR-M_R (Pr0769) Region: HygR, FRT Sequence file: RDB16056_A9K8c.seq ![]() |
>D05769A1_A9K8_1_HygR-M_R_A02_01_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN GNNCGANTCC TTGCGGTCCG AATGGGCCGA ACCCGCTCGT 61 CTGGCTAAGA TCGGCCGCAG CGATCGCATC CATGGCCTCC GCGACCGGCT GCAGAACAGC 121 GGGCAGTTCG GTTTCAGGCA GGTCTTGCAA CGTGACACCC TGTGCACGGC GGGAGATGCA 181 ATAGGTCAGG CTCTCGCTGA ATTCCCCAAT GTCAAGCACT TCCGGAATCG GGAGCGCGGC 241 CGATGCAAAG TGCCGATAAA CATAACGATC TTTGTAGAAA CCATCGGCGC AGCTATTTAC 301 CCGCAGGACA TATCCACGCC CTCCTACATC GAAGCTGAAA GCACGAGATT CTTCGCCCTC 361 CGAGAGCTGC ATCAGGTCGG AGACGCTGTC GAACTTTTCG ATCAGAAACT TCTCGACAGA 421 CGTCGCGGTG AGTTCAGGCT TTTTGGCCAA GGAAGTTCCT ATACTTTCTA GAGAATAGGA 481 ACTTCGGAAT AGGAACTTCT AGGTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC 541 GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG 601 GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG 661 GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC 721 GCCGCTACAG GGCGCGTGGG GANACCCCCT AGAGCCCCAG CTGGTTCTTT CCGCCTCANA 781 AGCCATAGAG CCCACCGCAT CCCCAGCATG CCTGCTATTG TCTTCCCNAT CCTCCCCCTT 841 GCTGTCCTGC CCCACCCCNC CCCCCCAGAA TANAATGANA CCTACTCNGA NAATGCGATG 901 CAATTTCCTC ATTTTATTAN GAAAGGANAG NGGGANTGGN ACCNNCCANG GNNNNNGNAN 961 NNNNNNGGGG NAGGGNNAAN NNNNNNANGG NNNGNNNCTA NNANNNNCAG NCNNNNNNNN 1021 NN // |
Primer: mCherry-M_F1 (Pr0767) Region: insert mid (mCherry, Cdt1_30-120) Sequence file: RDB16056_A9K8d.seq ![]() |
>D05769A1_A9K8_1_mCherry-M_F1_A06_03_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNNGAANAN NNCNTGGGCT GGGNNGCCTC CTCCGAGCGG 61 ANGTACCCCG ANGNNGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC 121 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG 181 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC 241 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG 301 TACAAGGGAT ATCCATCACA CTGGCGGCCG CTCGAGCCCA GCCCCGCCAG GCCCGCACTC 361 CGCGCCCCGG CCTCCGCTAC CAGTGGCAGC CGCAAGCGCG CCCGCCCGCC CGCCGCCCCC 421 GGACGCGACC AGGCCAGGCC ACCGGCCCGC AGGAGACTGC GGCTGTCGGT GGACGAGGTT 481 TCCAGCCCCA GTACCCCCGA GGCCCCAGAC ATCCCAGCCT GCCCTTCTCC GGGCCAGAAG 541 ATAAAGAAAT CCACCCCGGC AGCAGGTCAG CCGCCCCACC TGACATCCGC GCAGGACCAG 601 GACACCATCC AATTGGAAGG CCGTGGCTCT CTTCTCACTT GTGGCGATGT CGAAGAGAAT 661 CCTGGACCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC 721 GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGANGGCGAN 781 GCCACCTACN GCAAGCTGAN CCTGAANCTG ATCTGCACCA CCGGCAAGCT GCCCGNGCCC 841 TGGCCCACCC TCNNGANCAC NCNNGGCTAC NGNCNGCAGN GNNNCNCCCG CNACCCCGAN 901 CACNTGAAGC AGCACGANNN CTNCANGNNC GCCNTGCCCN NANNNNTANN NNCNNGNANC 961 GNANCATCNN NTNNNNNGNA NNNNNNNNNN // |
Primer: Cdt1_F1 (Pr0768) Region: insert mid (T2A, mVenus) Sequence file: RDB16056_A9K8e.seq ![]() |
>D05769A1_A9K8_1_Cdt1_F1_E06_15_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCNN ANGNNNNGGN NNNCNTCNAA TTGGAAGGCC GTGGCTCTCT 61 TCTCNCTTGT GGCGATGTCG AAGAGAATCC TGGACCCATG GTGAGCAAGG GCGAGGAGCT 121 GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT 181 CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGCTGAT 241 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGGGCTACGG 301 CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC 361 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA 421 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG 481 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG 541 CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT 601 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC 661 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC AGTCCAAGCT 721 GANCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC 781 CNGGATCACT CTCGGCATGG NCGANCTGTA CAANGGATAT CCATCACACT NNCGGCCGCT 841 CNANATGAAT CCCANTATGA AGCANAANNC AAGAAGAAAT CAAAGAGAAT ATAAAGAATA 901 NTTCNGNCCC NAGAANAANN NNNNANNANN ANTCANCCNN NNNNNNNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Mort, R.L., Fucci2a: a bicistronic cell cycle reporter that allows Cre mediated tissue specific expression in mice. Cell Cycle 13 (17): 2681-2696 (2014). PMID 25486356. [link to RRC of NBRP] |
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