RDB16748 - pCAGGS-ChRGR-Venus

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pCAGGS-ChRGR-Venus (#RDB16748)

Expression vector of organelle optogenetics channelrhodopsin.

Clone info.	Mammarian expression vector. Channelrhodopsin-green receiver (ChRGR) tagged with Venus at C-terminus, CAG promoter. Codon optimized for human.
Comment	Expression was confirmed by the depositor with immunostaining.
Vector backbone	pCAGGS (Plasmid)
Size of vector backbone	4.8 kb
Selectable markers	Amp^r (E. coli).
Growth conditions	LB + Amp, 37oC.
Gene/insert name	Chlamydomonas reinhardtii acop1 (channelopsin-1) cDNA Chlamydomonas reinhardtii cop4 (acop2) cDNA
Reference of insert sequence	LC415029 (DDBJ accession number)
Depositor\|Developer	Ishizuka, Toru \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Asano, T., Front. Neurosci. 12: 561, 2018). For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Asano, T., Front. Neurosci. 12: 561, 2018)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
提供同意書 (MTA, 営利目的用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Asano, T., Front. Neurosci. 12: 561, 2018)。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB16748	pCAGGS-ChRGR-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGGS-ChRGR-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB16748).

Reference section:

Asano, T., Igarashi, H., Ishizuka, T., Yawo, H., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB16748_A8Jnp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328) Region: intron, insert 5' Sequence file: RDB16748_A8Jne.seq
>16748_16748_B0A9S_pCAG-F_F04_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GGCGGGGTTC GGCTTCTGGC 61 GTGTGANCGG CGGCTCTANA NNCNCTGCTA ACCATGTTCA TGCCTTCTTC TTTTTCCTAC 121 AGCTCCTGGG CAACGTGCTG GTTATTGTGC TGTCTCATCA TTTTGGCAAA GAATTCGCCA 181 CCATGGCTCG GAGACCTTGG CTGCTGGCAC TGGCTCTGGC TGTCGCTCTG GCCGCTGGCT 241 CTGCTGGGGC TTCTACTGGA TCTGACGCAA CAGTGCCCGT CGCTACTCAG GACGGACCTG 301 ATTACGTGTT CCACAGGGCA CATGAGCGCA TGCTGTTTCA GACATCTTAT ACTCTGGAAA 361 ACAATGGCAG TGTCATCTGC ATTCCCAACA ATGGGCAGTG CTTCTGTCTG GCATGGCTGA 421 AGTCTAACGG CACCAATGCC GAGAAACTGG CCGCTAACAT CCTGCAGTGG ATTACATTCG 481 CCCTGTCCGC TCTGTGCCTG ATGTTTTACG GATATCAGAC CTGGAAGTCT ACATGTGGCT 541 GGGAGGAAAT CTACGTGGCT ACCATCGAGA TGATCAAGTT CATCATTGAA TACTTCCACG 601 AGTTTGACGA ACCTGNCNNG ATCTATAGCT CCAACGGGAA TAAGACTGTC TGGCTGANAT 661 NCNCTGAGTG GCTGCTGACC TGTCCAGTGA TCCTGATTCA TCTGAGCAAC CTGACTGGNC 721 TGGCCAACGA CTATAATAAG AGAANCCATG GGGGCTGCTG GTGANTGATA TCGGAACAAT 781 TGTCTGGGNN NNNNCANCAN NNNTNNCNAA AGGCTATGTG CGCGTCATCT TCTTTCTGAT 841 NGGNNCTGTG CTACNGGATC TNNACATTCT TTAANGNNNN CNNAANGNGT NCNTCGAGGC 901 NTNNNCNCNC AGTNCCTANN NNGNNNGGTG TANACNGGNN GNNCNCNNGN ANNGGCNTNN 961 NTGNTNNTTT GNGTNCNNGG GGNANGTNNC NNNTNCNGNT TNNTGNNGGN NNCCNNANGN 1021 ATNCNGGNNN NNTTNNNNCN NNNTNNNNTN NNNNNNNNN //
Primer: Venus-N_R (Pr0341) Region: Venus 5', insert 3' Sequence file: RDB16748_A8Jnf.seq
>D05919A3_A8Jn_1_Venus-N_R_B02_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NTTGTGNNCG TTTNNGTCGC CGNNCNNGCT CGNNCAGNNN 61 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGCGA CCGGTGGATC 121 CTTCCCAGTC CCTTTGTTCA CGGCTCCTGC CTCGGCTTCA TCTTCCACCA GTGTTTCCAC 181 CTCAATTTCG GTCCCGCCGA TATTCAGTTT GGTAGTCTTC CGAATATCGC CATGAATCAG 241 GATATGCTCG TGGATCAGCA CTCGCAGGTA GTGTCCCAGC AGCCCCCAGC AGTTTTTGCT 301 GGCCAGGTCC AGAATTGCAT GAGCGATGGC TGAATTAAAC TGGTTAATGT GGCCGAATCC 361 TTCGGGTCCC AGCAGAAACA GGATTGGGAA CATCCCCCAG GACACAAAGA ACAGCCAGGC 421 CATTCCAGTG ACCACCTGTC TACACCGCCC TTTAGGGACT GTGTGATATG CCTCGATGTA 481 CACCTTTGCA GCATTAAAGA ATGTGTAGAT CCCGTAGCAC AGGCCCATCA GAAAGAAGAT 541 GACGCGCACA TAGCCTTTTG ACAGGGCTGC TGTGGTGCCC CAGACAATTG TTCCGATATC 601 ACTCACCAGC AGCCCCATGG TTCTCTTATT ATAGTCGTTG GCCAGGCCAG TCAGGTTGCT 661 CAGATGAATC AGGATCACTG GACAGGTCAG CAGCCACTCA GCGTATCTCA GCCAGACAGT 721 CTTATTCCCG TTGGAGCTAT AGATCACGGC AGGTTCGTCA AACTCGTGGA AGTATTCAAT 781 GATGAACTTG ATCATCTCGA TGGTAGCCAC GTANATTTCN TCCCAGCCAC ATGTANACTT 841 CCAGGNCTGA NATCCGTAAA ACATCANGNA CAGANCGGNN NNGGCGAATG NNNTNNNNNG 901 CNNGNANGTT ANCNNCCANT TTNNNGNNNN NGGNNCCNNT NNGANTNNNN NNNNNN //
Primer: pCAGGS_R1 (Pr0614) Region: b-globin p(A), Venus 3' Sequence file: RDB16748_A8Jng.seq
>16748_16748_B0A9S_pCAGGS_R1_G04_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNTTGNNATG ANNNNAAATT TCCTTTATTA GCCAGAAGTC 61 AGATGCTCAA GGGGCTTCAT GATGTCCCCA TAATTTTTGG CAGAGGGAAA AAGATCTCAG 121 TGGTATTTGT GAGCCAGGGC ATTGGCCACA CCAGCCACCA CCTTCTGATA GGCAGCCTGC 181 ACCTGAGGAG TGAATTCTCA TTACTTGTAC AGCTCGTCCA TGCCGAGAGT GATCCCGGCG 241 GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT CGTTGGGGTC TTTGCTCAGG 301 GCGGACTGGT AGCTCAGGTA GTGGTTGTCG GGCAGCAGCA CGGGGCCGTC GCCGATGGGG 361 GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCCGCCGT CCTCGATGTT GTGGCGGATC 421 TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG TGATATAGAC GTTGTGGCTG 481 TTGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT CCTCCTTGAA GTCGATGCCC 541 TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT TCACCTCGGC GCGGGTCTTG 601 TAGTTGCCGT CGTCCTTGAA GAAGANGGTG CGCTCCTGGA CGTAGCCTTC GGGCATGGCG 661 GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC GGGCGAAGCA CTGCAGGCCG 721 TAGCCCAGGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA GCTTGCCGGT GGTGCAGATC 781 AGCTTCAGGG TCAGCTTGCC GTANGTGGNA TCGCCCTCGC CCTCGCCGGA NACGCTGAAN 841 TTGTGGNCGT TTACGTCGCC GTCCAGCTCG ANCANGANGG GCACCACCCC GGTGAANAGC 901 TCCNCGCCCT TGNTCNNCAN GGNNNNNANC GGNNGNNNCT TNCNNGNCCC TTTGNTCNNN 961 NNTNCNGNCN CNGCTTCNTN NNNNNNCANN GNTTCNNNCN NNNNTTCGGN CCNNNNNNNN 1021 NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Asano, T., Organelle Optogenetics: Direct Manipulation of Intracellular Ca2+ Dynamics by Light. Front. Neurosci. 12: 561 (2018). PMID 30174581. [link to RRC of NBRP]