Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAG-NexxoR

Selection plasmid for the positive clone of CRISPR/Cas9 system in the culture cells.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, description of “This construct was created based on the pCAG-EGxxFP (Mashiko, O., Sci. Rep. 3: 3355, 2013)” is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とする。利用者は研究成果の公表に当たって、本件リソースがpCAG-EGxxFP (Mashiko, O.,Sci. Rep. 3: 3355, 2013) を基に作成したことを記すこと。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB16803_A8Llp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328) Region: CAG pro,insert 5'(NexxoR-N 5') Sequence file: RDB16803_A8Llc.seq
>16803_16803_B2LrF_pCAG-F_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGNAGGG CGGGGTTCGG CTTCTGGCGT    61 GTGACCGGCG GCTCTAGCCT CTGCTAACCA TGTTCATGCC TTCTTCTTTT TCCTACAGCT   121 CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAATCTA GAGCCGCCAT   181 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG   241 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC   301 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA   361 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT   421 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA   481 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG   541 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT   601 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA   661 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA TGCCCGACGG   721 CGAGGATCTC GTCGTGACCC ATGGCGTGAG GATCCGCTAG CCTGCAGGTC GACGAATTCG   781 ATATCTAGAA GACCGACCTG TCCGGTGCCC TGAATGANCT GCAAGACGAG GCAGCGCGGC   841 TATCGTGNCT GGNCNCGACG GGCGTTCCTT GCGCNGCTGT GCTCGACGTT GTCACTGAAN   901 CNGNAAGGGN NTGGCTGCTA TTGNNNNAAN TGNCNGGGCA GGATNNNCTG TNNTCTCNNC   961 TNGNTCCTGC CNANAANNNA TCCATCANGN NTGANGNNAN GCNNCNGNNG CATACNCNTG  1021 ATNNGGNNAN NNNNCCNNTC NANNNNNNNG CGAAANNTNN NANNNNNNCG ANNNNNTNNN 1081 NNNNNNGNAN NNNNNNNNN //
Primer: pCA-bgter (Pr0066) Region: insert 3'(NexxoR-C) Sequence file: RDB16803_A8Lld.seq
>16803_16803_B2LrF_pCA-bgter_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNTTTGNNN NAGGGNNNAA GNNCTCAGTG GTATTTGTGA GCCAGGGCAT    61 TGGCCACACC AGCCACCACC TTCTGATAGG CAGCCTGCAC CTGAGGAGTC TCGAGCCCGC   121 TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT   181 ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG   241 GGTAGCCAAC GCTATGTCCT GATAGCGGTC CGCCACACCC AGCCGGCCAC AGTCGATGAA   301 TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC   361 GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC   421 GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT   481 ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG   541 CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG   601 AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC   661 AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGNNCAGCC ACGATAGCCG   721 CGCTGCCTCG TCTTGNAGTT CANTCANGGC ACCGGANAGG NCGGTCTTCT AGATATCGAA   781 NTCGTCGANC TGCANGCTAG CGNATCCTCA CGCCNTGGGT CACGACGANA TNCTCGCCGN   841 CNGGNATGCT CGCCNTGAGC CTGGCGAANA GTTCGGCTGG NGCGAGCCCC NGANGNTCTT   901 CGTCCNNANC ATCCTGNNCN ANNANANNGG NTTNNNTCCN ANNANNNNNN NNCTCNATGN 961 NANGNTNCNC NNNNNGNCNN NNGGNNNNNN ANNNNNNNNN N //
Primer: xoR-M_R (Pr 1277) Region: insert mid(NexxoR-C,MCS,NexxR-N 3') Sequence file: RDB16803_A8Lle.seq
>16803_16803_B2LrF_xoR-M_R_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC    61 GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC   121 CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC   181 CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG   241 AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT   301 TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA   361 CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTCTA   421 GATATCGAAT TCGTCGACCT GCAGGCTAGC GGATCCTCAC GCCATGGGTC ACGACGAGAT   481 CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT   541 GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC   601 GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA   661 GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA   721 GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA   781 CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT   841 CGTCTTGNAG TTCATTCNNG GCACCGGACA GGTCGGTCTT GANAAAAAGA ACCGGGCGCC   901 CCTGCGCTGA NAGCCGGAAC ACGGCGGCNT CNNANCANCC GANTGNCTGN TGTGNCCNGT   961 CNTANNNNAN TAGCCTCTNN ACCCAAGCGG NCNGNANAAN NTGNGNGNNN TCCNTCNTNG 1021 NNCNNTCNNN GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl