Please review the QC test results indicated by icon below as well as clone information before placing your order.

pNEF-BOS-EX

Mammalian expression vector based on human EF-1 alpha promoter with G418-resistant gene.

Sequence (full)	RDB18970zzk01.seq provided by the depositor
Remarks, protocol and/or map (png)	RDB18970_map.png

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.

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Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Uetsuki, T. et al., J. Biol. Chem., 264 (10): 5791-5798, 1989), (Mizushima, S. and Nagata, S., Nucleic Acids Res., 18 (17): 5322, 1990)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18970_B1Dkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724) Region: SV40 pro_ori.1, EF-1alpha pro Sequence file: RDB18970_B1Dka.seq
>D07990B1_B1Dk_2_lacIpro-B_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNNNNNCNG TGNNCGANGA ANCGGAANAN CGCCCAATAC    61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC   121 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG   181 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT   241 AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTTAAT TCCCTCCCCA   301 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA   361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA   421 CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG   481 TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTTTGCAA AGATGGATAA   541 AGTTTTAAAC AGAGAGGAAT CTTTGCAGCT AATGGACCTT CTAGGTCTTG AAAGGAGTGG   601 GAATTGGCTC CGGTGCCCGT CAGTGGGCAG AGCGCACATC GCCCACAGTC CCCGAGAAGT   661 TGGGGGGAGG GGTCGGCAAT TGAACCGGTG CCTAGAGAAG GTGGCGCGGG GTAAACTGGG   721 AAAGTGATGT CGTGTACTGG CTCCGCCTTT TTCCCGAGGG TGGGGGANAA CCGTATATAA   781 GTGCAGTANT CGCCGNGAAC GTTCTTTTTT CGCAACGGGT TTGCCGCCNG AACACANGNA   841 AGTGCCNNGN GNGGTNNCCG CGGGCCTGNN CNCTTNACNG NNTATGGNCC TTNCNNGNCN 901 NNNNNNNNTN NCACNNNNNN NNAGNACNNN ATTNNNNNTN NNNNNNNNN //
Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro,hG-CSF pA Sequence file: RDB18970_B1Dkb.seq
>D07990B1_B1Dk_2_EF1a-C_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTT TTGNNTTTGG NNCTTGGTTC NTTCTCNAGC CTCNGACAGT    61 GGTTCAAAGT TTTTTTCTTC CNTTTCAGGT GTCGTGAGGA ATTCGGTACC GCGGCCGCCC   121 GGGGNTCCGC TAGCGTCGAC TCTAGAGTGA GGGTCCCCAC CTGGGACCCT TGAGAGTATC   181 AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT TTAAACAGCA GTGTTCCCCA   241 TCTGGGTCCT TGCACCCCTC ACTCTGGCCT CAGCCGACTG CACAGCGGCC CCTGCATCCC   301 CTTGGCTGTG AGGCCCCTGG ACAAGCAGAG GTGGCCAGAG CTGGGAGGCA TGGCCCTGGG   361 GTCCCACGAA TTTGCTGGGG AATCTCGTTT TTCTTCTTAA GACTTTTGGG ACATGGTTTG   421 ACTCCCGAAC ATCACCGACG TGTCTCCTGT TTTTCTGGGT GGCCTCGGGA CACCTGCCCT   481 GCCCCCACGA GGGTCAGGAC TGTGACTCTT TTTAGGGCCA GGCAGGTGCC TGGACATTTG   541 CCTTGCTGGA CGGGGACTGG GGATGTGGGA GGGAGCAGAC AGGAGGAATC ATGTCAGGCC   601 TGTGTGTGAA AGGAAGCTCC ACTGTCACCC TCCACCTCTT CACCCCCCAC TCACCAGTGT   661 CCCCTCCACT GTCACATTGT AACTGAACTT CAGGATAATA AAGTGTTTGC CTCNNNNNAA 721 AAAANANNNN NNAAAAAAAN ANNANNNNNN ANNNNNNNNN N //
Primer: Neo-M_R (Pr0145) Region: NeoR,SV40 pro_ori.2 Sequence file: RDB18970_B1Dkc.seq
>D07990B1_B1Dk_2_Neo-M_R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNCNNNNN NCTTNNGTGN NNACGTCNNN CACAGCTGCG    61 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCCTG CAGTTCATTC   121 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG   181 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC   241 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT   301 CCTCATCCTG TCTCTTGATC AGATCCGAAA ATGGATATAC AAGCTCCCGG GAGCTTTTTG   361 CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG   421 GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG   481 GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC   541 TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC   601 CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG   661 GACTTTCCAC ACCCTAACTG ACACACATTC CACAGGGATC TGATATCCAT ATGCCTGCAN   721 GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA AGGNGGNNGT GCTGCAAGGC   781 GATTAAGTTG GGTAACGCCA GGGTTTNCCC ANTCACGANG TTGTAAACGA NNNNNANNGN 841 AANTNNNGNN NNNNNNNN //
Primer: AmpR_F (Pr0962) Region: AmpR,ori Sequence file: RDB18970_B1Dkd.seq
>D07990B1_B1Dk_2_AmpR_F_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCGC TGNNNNAGGT GCCTCACTGA TTAAGCATTG    61 GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA   121 ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG   181 TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA   241 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT   301 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG   361 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA   421 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG   481 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA   541 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC   601 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA   661 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC   721 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG   781 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC   841 CTTTTTACGG NTCCTGNNCT TTTGNTGGCC TTTTGNTCAC ATGNTCTTNC CNGCGTTATC   901 CCCTGATTCT GTGGATAACC GTATTANCNN CTTTGAGTGA NCTGANNCCG CTCGCCGCAN   961 CCGAACGANC GANCGCANCG AGNNANTGAN CNANGNANNC NNANNANCNN CCNNNACNCA 1021 ANNCNNNNCN NNCCCNNNNN NNNNN //
Primer: pEFbosNeo-M_F1 (Pr0913) Region: hG-CSF pA Sequence file: RDB18970_B1Dke.seq
>D07990B1_B1Dk_2_pEFbosNeo-M_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NTGNNNNNNN NNCCCTGCAT CCCCTTGGCT    61 GTGAGGCCCC TGGNCAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT GGGGTCCCAC   121 GAATTTGCTG GGGAATCTCG TTTTTCTTCT TAAGACTTTT GGGACATGGT TTGACTCCCG   181 AACATCACCG ACGTGTCTCC TGTTTTTCTG GGTGGCCTCG GGACACCTGC CCTGCCCCCA   241 CGAGGGTCAG GACTGTGACT CTTTTTAGGG CCAGGCAGGT GCCTGGACAT TTGCCTTGCT   301 GGACGGGGAC TGGGGATGTG GGAGGGAGCA GACAGGAGGA ATCATGTCAG GCCTGTGTGT   361 GAAAGGAAGC TCCACTGTCA CCCTCCACCT CTTCACCCCC CACTCACCAG TGTCCCCTCC   421 ACTGTCACAT TGTAACTGAA CTTCAGGATA ATAAAGTGTT TGCCTCCNAA AAAAAAAAAA   481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 541 AAAAAAAAAN NNNNNNNTNN NNNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro, SV40 pA,NeoR Sequence file: RDB18970_B1Dkf.seq
>D07990B1_B1Dk_2_Amp_R_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT GANCATTTAT CNGGGTTATT GTCTCNTGAG CGGATACATA    61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG   121 CCACCTGACG TCGACGGTAT ACAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA   181 CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT   241 TTGTAACCAT TATAAGCTGC AATAAACAAG TTGGGGTGGG CGAAGAACTC CAGCATGAGA   301 TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT   361 TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG   421 GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA   481 TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC   541 CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG CGGTCCGCCA   601 CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG   661 GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG CGCGCCTTGA   721 GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT   781 CGACAAGANC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTNGGTGGT   841 CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCN NNCATGATGG   901 ATANTTTCTC GNCNNGANCN AGGNGAGATG ANAGGANATN CTGCCCNNGN NCNTCNCCCN   961 ANANNANCCA GTCCCTTCCN NCTTCNGTGA NNNCGTCNAN CACAGCTGCN NNANNNNNNG 1021 CCCNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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