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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-BOS-EX

Mammalian expression vector with human EF-1a.

Catalog number RDB07940
Resource name pEF-BOS-EX
Clone info. Mammalian expression vector with human EF-1a. Related vector pEF-BOS-EX (RDB07940) is also available.
Comment Please refer Prof. Nagata's web page for datails (http://biochemi.ifrec.osaka-u.ac.jp/english/research/pef-bosex.html).
Vector backbone pEF-BOS-EX (Plasmid)
Size of vector backbone 5.277 bp
Selectable markers Amp^r
Depositor|Developer Nagata, Shigekazu |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB07940zzk04.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB07940.pdf
            Remarks, protocol and/or map (png) RDB07940_map.png

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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            MTA, for use for not-for-profit academic purpose [Word]
            Please visit Information of Request for Distribution.[link] 
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって、長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB07940 pEF-BOS-EX DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pEF-BOS-EX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07940).

            Reference section:

            Mizushima, S., Nagata, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Mammalian Expression Vector, pEF-BOS series (English text)
            Featured content 哺乳動物細胞用の遺伝子発現ベクター pEF-BOSシリーズ (Japanese text)
            Featured content Bacteria host strains (en) (English text)
            Featured content Bacteria host strains (ja) (Japanese text)
            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19703_B1Ifp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EF1a-C_F (Pr0168)
            Region: EF-1 alpha pro,MCS,hG-CSF 3’ UTR+pA
            Sequence file: RDB19703_B1Ifg.seq check
            >RDB07940_19703_B1If_1_EF1a-C_F_D01_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNT NNNNNNNNNT GGNNNNNNGN NNNNNCTCNN NNCTCNNNNN
               61 NTGGTTCAAA GTTTTTTTCT TCCATTTCNN GTGTCGTGAG GAATTCGGTA CCGCGGCCGC
              121 CCGGGGATCC GCTAGCGTCG ACTCTAGAGT GAGGGTCCCC ACCTGGGACC CTTGAGAGTA
              181 TCAGGTCTCC CACGTGGGAG ACAAGAAATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC
              241 CATCTGGGTC CTTGCACCCC TCACTCTGGC CTCAGCCGAC TGCACAGCGG CCCCTGCATC
              301 CCCTTGGCTG TGAGGCCCCT GGACAAGCAG AGGTGGCCAG AGCTGGGAGG CATGGCCCTG
              361 GGGTCCCACG AATTTGCTGG GGAATCTCGT TTTTCTTCTT AAGACTTTTG GGACATGGTT
              421 TGACTCCCGA ACATCACCGA CGTGTCTCCT GTTTTTCTGG GTGGCCTCGG GACACCTGCC
              481 CTGCCCCCAC GAGGGTCAGG ACTGTGACTC TTNNNNNNGC CAGGCAGGTG CCTGGACATT
              541 TGCCTTGCTG GACGGGGACT GGGGATGTGG GAGGGAGCAG ACAGGAGGAA TCATGTCAGG
              601 CCTGTGTGTG AAAGGAAGCT CCACTGTCAC CCTCCACCTC TTCACCCCCC ACTCACCAGT
              661 GTCCCCTCCA CTGTCACATT GTAACTGAAC TTCAGGATAA TAAAGTGTTT GCCTCCNNNN
            721 NNNAANNAAA NNNNNAANNA NNNNNNNNNA NNNNNNNNNN
            //
            Primer: f1ori_R3 (Pr0142)
            Region: hG-CSF 3’ UTR+pA
            Sequence file: RDB19703_B1Ifh.seq check
            >07940_19703_B1If_1_f1ori_R3_E04_13_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCC GCACAGATGC GTAAGGAGAA AATACCGCAT
               61 CAGGCGCCAT TCNNNNNNNA NGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC
              121 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC
              181 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGAAATTC GGTTTTTTTT
              241 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
            301 TTTTTTTTTT TTTNNNNNNN NNN
            //
            Primer: AmpR_F (Pr0962)
            Region: AmpR 3',ori
            Sequence file: RDB19703_B1Ifi.seq check
            >RDB07940_19703_B1If_1_AmpR_F_A01_01_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCG CTGNNNNAGG TGCCTCACTG ATTAAGCATT
               61 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT
              121 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC
              181 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG
              241 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG
              301 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA
              361 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA
              421 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA
              481 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC
              541 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA
              601 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA
              661 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC
              721 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC
              781 GTCGATTTTT GTGATGCTCG TCNNNNGGNC GGANCCTATG GAAAAACGCC AGCAACGCGG
              841 CCTTTTTACG GTTCCTGNNC TTTTGCTGGC CTTTTGCTCA CATGTNCTTT CCTGCGTTAT
              901 CCCCNGATTC TGTGGATAAC CGTATNNNCG CCTTTGANTG AGCTGATNCC GCTCGNCNGC
              961 ANCNNANNNA NCNANCGCAN CGNNNNAGTG ANCNANNNNN CNNANNNNCG CCNNANNNNN
            1021 NANNNNNNNN NN
            //
            Primer: lacIpro-B_F (Pr0724)
            Region: SV40 pro_ori, EF-1 alpha pro
            Sequence file: RDB19703_B1Ifj.seq check
            >RDB07940_19703_B1If_1_lacIpro-B_F_B01_02_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNAGTGAGCG AGGNAGCGGA AGANCGCCCA
               61 ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG GCACGACAGG
              121 TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA GCTCACTCAT
              181 TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG AATTGTGAGC
              241 GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT TAATTCCCTC
              301 CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC
              361 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG
              421 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA
              481 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTTT GCAAAGATGG
              541 ATAAAGTTTT AAACAGAGAG GAATCTTTGC AGCTAATGGA CCTTCTAGGT CTTGAAAGGA
              601 GTGGGAATTG GCTCCGGTGC CCGTCAGTGG GCAGAGCGCA CATCGCNNNN NNTCCCCGAG
              661 AAGTTGGGGG GAGGGGTCGG CAATTGAACC GGTGCCTAGA GAAGGTGGCG CGGGGTAAAC
              721 TGGGAAAGTG ATGTCGTGTA CTGGCTCCGC CTTTTNNNNA NGGTGGGGGA NAANCGTATA
              781 TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG GTTTGCCGCC ANAACACAGG
              841 TAAGTGNCGT GTGTGGNNTN CCCGCNGGCC TGGCCTCTTT ACGGGTNANG GCCCTTGCGN
              901 GCCNTNGANT TACNTNCNNC CTNNNNTGCA NNACGTGATT CNTNGNNTNN NNNANCTTNN
              961 NGGGTTNGGA AGTGGGTGGG NNNANNTTCN ANGNNCNTNN CNCNTNANNN NNCNNCCNTN
            1021 NGCCNCNNNC NTNGNANNNN NNNGNNNNNG NNNNNNGNNN NNNNNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori, EF-1 alpha pro
            Sequence file: RDB19703_B1Ifk.seq check
            >07940_19703_B1If_1_SV40pro_ori_F_H06_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNCGC CTCGGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
               61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTT TGCAAAGATG GATAAAGTTT TAAACAGAGA
              121 GGAATCTTTG CAGCTAATGG ACCTTCTAGG TCTTGAAAGG AGTGGGAATT GGCTCCGGTG
              181 CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG GGAGGGGTCG
              241 GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT GATGTCGTGT
              301 ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA GTAGTCGCCG
              361 TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC GTGTGTGGTT
              421 CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT ACTTCCACCT
              481 GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG GAGAGTTCGA
              541 GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG CCTGGGCGCT
              601 GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT TTCGATAAGT
              661 CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG CAAGATAGTC
              721 TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC GCGGGCGGCG
              781 ACNGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA GCGCGGCCAC
              841 CGANAATCGG ANGGNNGTAG TCTCAAGCTG GCCGGNCNGC TCTGGTGCCN GGCCTCNCGC
              901 CGCCNNGTAT CGCCCCGCCC TNNNNGCAAN GCTGGNCCNG NCNGNANCAG TTNCNNGANC
            961 NNAAAGNNNG NCNCTNNCNN NNCCNNNNNN NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Mizushima, S., pEF-BOS, a powerful mammalian expression vector. Nucleic Acids Res., 18 (17): 5322 (1990). PMID 1698283. [link to RRC of NBRP]
            reference Yanagihashi, Y., Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis. Proc. Natl. Acad. Sci. U.S.A. 114 (33): 8800-8805 (2017). PMID 28768810. [link to RRC of NBRP]
            reference Murai, K., Myeloid-specific transcriptional activation by murine myeloid zinc-finger protein 2. Proc. Natl. Acad. Sci. U.S.A. 95 (7): 3461-3466 (1998). PMID 9520388.

            2024.03.26

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