Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(Y441A)

Retroviral expression vector of human KLF4, mutant Y441A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19006_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(Y441A)05') Sequence file: RDB19006_B1Eca.seq
>D07716A3_B1Ec_3_MMLV-pol_F_B03_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN ATCGCAGCTT GGNNACACGC CGCCNNCGTG AAGGCTGCCG    61 ACCCCGGGGG TGGACCATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCCAG   121 TGTGGTGGTA CGGGAATTCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA   181 CATCGACTAC AAGGATGACG ATGACAAGGC TGTCAGCGAC GCGCTGCTCC CATCTTTCTC   241 CACGTTCGCG TCTGGCCCGG CGGGAAGGGA GAAGACACTG CGTCAAGCAG GTGCCCCGAA   301 TAACCGCTGG CGGGAGGAGC TCTCCCACAT GAAGCGACTT CCCCCAGTGC TTCCCGGCCG   361 CCCCTATGAC CTGGCGGCGG CGACCGTGGC CACAGACCTG GAGAGCGGCG GAGCCGGTGC   421 GGCTTGCGGC GGTAGCAACC TGGCGCCCCT ACCTCGGAGA GAGACCGAGG AGTTCAACGA   481 TCTCCTGGAC CTGGACTTTA TTCTCTCCAA TTCGCTGACC CATCCTCCGG AGTCAGTGGC   541 CGCCACCGTG TCCTCGTCAG CGTCAGCCTC CTCTTCGTCG TCGCCGTCGA GCAGCGGCCC   601 TGCCAGCGCG CCCTCCACCT GCAGCTTCAC CTATCCGATC CGGGCCGGGA ACGACCCGGG   661 CGTGGCGCCG GGCGGCACGG GCGGAGGCCT CCTCTATGGC AGGGAGTCCG CTCCCCCTCC   721 GACGGCTCCC TTCAACCTGG CGGACATCAA CGACGTGAGC CCCTCGGGCG GCTTCGTGGC   781 CGANCTCCTG CGGCCAGAAT TGGANCCGGT GTACATTCCG CCGCANCAGC CGCAGCCGCC   841 AGGNGGCGGG CTGATGGGCA AGTNCGNGCT GAANGNGTCG CTGANCGCCC CTGGNAGCGA   901 GNACNGNAGC CCGNCNGNCA TCNGCGTCAG CAAAGGCAGC CCNGANNNNA NNNNCCCGGN 961 GGNGNNGGCG NCNNACNACN NNNGNNNNNN NCGCACNNNN CCNNNNNNNN NN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(Y441A) 3') Sequence file: RDB19006_B1Ecb.seq
>D07716A3_B1Ec_3_pMXs-3LTRup_R2_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NATCGTCGNN NNCTGTGCTG GCGGCCGCTT AAAAATGCCT    61 CTTCATGTGT AAGGCGAGGT GGTCCGACCT GGAAAATGCT CGGTCGCATT TTTGGCACTG   121 GAACGGGCGG TGCCCCGTGT GTTTACGGTA GTGCCTGGTC AGTTCATCTG AGCGGGCGAA   181 TTTCCATCCA CAGCCGTCCC AGTCACAGTG GTAAGGTTTC TCACCTGTGT GGGTTCGCAG   241 GTGTGCCTTG AGATGGGAAC TCTTTGTGGC GGTTTTGCCG CAGCCCGCGT AATCACAAGT   301 GTGGGTGGCG GTCCTTTTCC GGGGCCACGA TCGTCTTCCC CTCTTTGGCT TGGGCTCCTC   361 TGGCATGCAG GAACCGGGTG GCATGAGCTC TTGGTAATGG AGCGGCGGGA CTTGCGGCTG   421 CATCTGATCG GGCAGGAAGG ATGGGTAATT GGGCCCCGGG TGGGGATGGA AGCCGGGAGG   481 AAGCGGCAGG GCAGGGTGAC AGTCCCTGCT GCTCAGCACT TCCTCAAGAC CCAGGGTCGG   541 GGTAGTCCTG CTGGGGAGCT GCCGCCCCAG GGGGAAGTCG TGTGCAGCCG GCCGGTGGCC   601 ATTGCTGAGA GGGGGTCCAG CGCCCAAGTG GGTGCACGAA GAGACCGCCT CCTGCTTGAT   661 CTTGGGGCAC GTGCGCGGCG GCCCGCCGTT GTAGGGCGCC ACCACCACCG GGTGGCTGCC   721 GTCAGGGCTG CCTTTGCTGA CGCTGATGAC CGACGGGCTG CCGTACTCGC TGCCAGGGGC   781 GCTCANCGAC GCCTTCAGCA CGAACTTGCC CATCAGCCCG CCACCTGGCG GCTGCGGCTG   841 CTGCGGCGGA NTGTACACCG NGNCCNATTC TGGNCGCNNG ANCTCGGCCA CNAANCCGCC   901 CNANGGGCTC ACGTCGTTGA TGNNCGCCAN GTTNNANGGN ANCCGNNNNA NGGGGANCNN   961 NNTNCNTGNC NTANANNANN NNNNNNCCCG NGNCNNNCNG NNGNNNCNNC NNGNNNNNNN 1021 CNNNNNNNNN N //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19006_B1Ecc.seq
>D07716A3_B1Ec_3_gag-N_R_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNNT TTTNNNANNN NNANNGAAAC ACAGTCAGAC    61 AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC GGCGCGGCTT CGGTCCCAAA   121 CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG GTTCTCGTCT CCTACCAGAA   181 CCACATATCC CTCCTATTAG GGGGGTGCAC CAAAGAGTCC AAAACGATCG GGATTTTTGG   241 ACTCAGGTCG GGCCACAAAA ACGGCCCCCG AAGTCCCTGG GACGTCTCCC AGGGTTGCGG   301 CCGGGTGTTC CGAACTCGTC AGTTCCACCA CGGGTCCGCC AGATACAGAG CTAGTTAGCT   361 AACTAGTACC GACGCAGGCG CATAAAATCA GTCATAGACA CTAGACAATC GGACAGACAC   421 AGATAAGTTG CTGGCCAGCT TACCTCCCGG TGGTGGGTCG GTGGTCCCTG GGCAGGGGTC   481 TCCCGATCCC GGACGAGCCC CCAAATGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA   541 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA   601 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCGC CGAGTGAGGG   661 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA   721 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA   781 ACATCTGATG GTTCTCTAGA AACTGCTGAN GGCTGGACCG CATCTGGNGA NCATCTGTTC   841 TTGGCCCTGA NCNNGGGCNN GAACTGCTTA CCACAGATAT CCNGNTTGGN CCATATTCNG   901 CTGTTNCNTC TGTTCTTGGN CNNGANCCGG GGCNNGNANC TGCTNANCAC AGATATCCNG   961 NTNNGNNCCN TANTNNNNCN GNTNNNATNN NNNNCNNGAN CNTNGNTNNN NNNNTTTNNN 1021 NNNNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19006_B1Ecd.seq
>D07716A3_B1Ec_3_lacIpro-B_F_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NANCGNNTCN GTGAGCGAGG AANCGGAAGA GCGCCCAATA    61 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG   121 TAAATACTTA AGAAAAAAAA CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC   181 GCTGACGGGT AGTCAATCAC TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC   241 GGATGCAACT GCAAGAGGGT TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG   301 AGGACTGGCG CCCCGAGTGA GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC   361 GCGCGAACAG AAGCGAGAAG CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT   421 CAGGTCCTTG GGGCACCCTG GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA   481 CCGCATCTGG GGACCATCTG TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA   541 TATCCTGTTT GGCCCATATT CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA   601 ACTGCTTACC ACAGATATCC TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT   661 TGATCTGAAC TTCTCTATTC TCAGTTATGT ATTTTTCCAT GCCTTGCAAA ATGGCGTTAC   721 TTAAGCTAGC TTGCCAAACC TACAGGTGGG GTCTTTCATT CCCCCCTTTT TCTGGAGANT   781 AAATAAAATC TTTTATTTTA TCGTCGANCA CTGTGCTGGC GGCCGCTTAA AAATGCCTCT   841 TCATGTGTAA NGNGAGGTGG NCCGACCTGN AAAATGCTCN GNCGCATTTT NGGCACTGGA   901 ACGGGCGGNG NCCCGNGNGT TTANGGNANT GNCNNNNCAG TTCATCTGAN CNGGNNANNT 961 TCCNTCCNCA NCNNNCCNAN NCACANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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