Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(T442A)

Retroviral expression vector of human KLF4, mutant T442A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19007_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(T442A) 5') Sequence file: RDB19007_B1Eca.seq
>D07716A4_B1Ec_1_MMLV-pol_F_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG GNATCGNANC TNGGNNACNC GCCGCCCACG TGAAGGCTGC    61 CGACCCCGGG GGTGGNNCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC   121 AGTGTGGTGG TACGGGAATT CATGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT   181 GACATCGACT ACAAGGATGA CGATGACAAG GCTGTCAGCG ACGCGCTGCT CCCATCTTTC   241 TCCACGTTCG CGTCTGGCCC GGCGGGAAGG GAGAAGACAC TGCGTCAAGC AGGTGCCCCG   301 AATAACCGCT GGCGGGAGGA GCTCTCCCAC ATGAAGCGAC TTCCCCCAGT GCTTCCCGGC   361 CGCCCCTATG ACCTGGCGGC GGCGACCGTG GCCACAGACC TGGAGAGCGG CGGAGCCGGT   421 GCGGCTTGCG GCGGTAGCAA CCTGGCGCCC CTACCTCGGA GAGAGACCGA GGAGTTCAAC   481 GATCTCCTGG ACCTGGACTT TATTCTCTCC AATTCGCTGA CCCATCCTCC GGAGTCAGTG   541 GCCGCCACCG TGTCCTCGTC AGCGTCAGCC TCCTCTTCGT CGTCGCCGTC GAGCAGCGGC   601 CCTGCCAGCG CGCCCTCCAC CTGCAGCTTC ACCTATCCGA TCCGGGCCGG GAACGACCCG   661 GGCGTGGCGC CGGGCGGCAC GGGCGGANGC CTCCTCTATG GCAGGGAGTC CGCTCCCCCT   721 CCGACGGCTC CCTTCNACCT GGCGGACATC AACGACGTGA GCCCCTCGGG CGGCTTCGTG   781 GCCGANCTCC TGCGGCCAGA ATTGGANCCG GTGTACNTTC CGCCGCANCA NCCGCAGNCG   841 CCAGGTGGCG GGCTGANGGG NAAGTTCGTG CTGAANGNGT CNCTGANCNN CCCNNNNANC   901 GAGNACNGNA GCCCGNNNGN CNTCAGCGTC ANCAAANGNA GCNNNGNNNN NANCCNCCCN 961 GNNGGNNGNG NNNNNNNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(T442A) 3') Sequence file: RDB19007_B1Ecb.seq
>D07716A4_B1Ec_1_pMXs-3LTRup_R2_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTNN NCGTCGNNNN CTNNNNNGGC GGCCGCTTAA AAATGCCTCT    61 TCATGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA   121 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGAATT   181 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT   241 GTGCCTTGAG ATGGGAACTC TTTGCGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT   301 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG   361 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA   421 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA   481 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG   541 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT   601 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT   661 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT AGGGCGCCAC CACCACCGGG TGGCTGCCGT   721 CAGGGCTGCC TTTGCTGANG CTGATGANCG ACGGGCTGCC GTACTCGCTG CCANGGGCGC   781 TCANCGACGC NTTCNGCACG AACTTGNCCN TCAGCCCGCC ACCNNNNNGC TGCNGCTGCT   841 GCNGCGGAAN GNACACCGGG NNNNANTCTG GNCNNNNGAN NNCGNNNNCN AAGNCNNCCN   901 ANGGGCTCAC NNCNNNNNNG NNNNNNANNN NNNANNGNAN CCNNNNNNNG GGNNNNNNNN 961 NN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19007_B1Ecc.seq
>D07716A4_B1Ec_1_gag-N_R_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN GNNNNNNNNN TTNNGANNAA TACAGAAACN CAGTCAGACA    61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC   121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC   181 CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA   241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC   301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA   361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA   421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT   481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG   541 AGGAGACCCT CCCAAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT   601 TGGATACACG GGTACCCGGG CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG   661 TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA   721 CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCANG TCCTTGGGGC ACCCTGGAAA   781 CATCTGATGG TTCTCTAGAA ACTGCTGANG GCTGGNNCGC ATCTGGGGAN NNTCTGTTCT   841 TGGCCCTGAN CCNGGGCANG ANNTGCTTAN NACAGATATN CNGTTTGGCC CNTATTCAGC   901 TNNNNCNTCN GNNCTNGGNC CNNNNCCNGG GNNNNNNNNG CNTANNNCAN ATATNCNNNT 961 TGGNNNNNNN NNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19007_B1Ecd.seq
>D07716A4_B1Ec_1_lacIpro-B_F_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNCGNNNCNG TGANCGAGGA AGCGGAAGAN CGCCCNATAC    61 GCAAACCGCC TCTCCCCNCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC   481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT   541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA   601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT   661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT   721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA   781 AATAAAATCT TTTATTTTAT CGTCGANNAC TGTGCTGGCG GCCGCTTAAA AATGCCTCTT   841 CATGTGTAAN GCGAGGTGNN NNNANNNGGA AAATGNTCNG TCGNNNTTTT TGGCACTGGA   901 ACGGGCGGNG CCCCGNGTGT TTACGGNANN GNCTGGNCAN NNCATCTGAN CGGGCGANNT 961 TNCNTCCNNN NCNNNCCNNN NCNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

GNP_filter3_RDBDEP_html_230702.pl