Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(K443A)

Retroviral expression vector of human KLF4, mutant K443A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19008_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(K443A) 5') Sequence file: RDB19008_B1Eca.seq
>D07716A5_B1Ec_1_MMLV-pol_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN ATCGCANCTT GGNNACACGC CGCCCACGTG AAGGCTGCCG    61 ACCCCGGGGG TGGANCATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCCAG   121 TGTGGTGGTA CGGGAATTCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA   181 CATCGACTAC AAGGATGACG ATGACAAGGC TGTCAGCGAC GCGCTGCTCC CATCTTTCTC   241 CACGTTCGCG TCTGGCCCGG CGGGAAGGGA GAAGACACTG CGTCAAGCAG GTGCCCCGAA   301 TAACCGCTGG CGGGAGGAGC TCTCCCACAT GAAGCGACTT CCCCCAGTGC TTCCCGGCCG   361 CCCCTATGAC CTGGCGGCGG CGACCGTGGC CACAGACCTG GAGAGCGGCG GAGCCGGTGC   421 GGCTTGCGGC GGTAGCAACC TGGCGCCCCT ACCTCGGAGA GAGACCGAGG AGTTCAACGA   481 TCTCCTGGAC CTGGACTTTA TTCTCTCCAA TTCGCTGACC CATCCTCCGG AGTCAGTGGC   541 CGCCACCGTG TCCTCGTCAG CGTCAGCCTC CTCTTCGTCG TCGCCGTCGA GCAGCGGCCC   601 TGCCAGCGCG CCCTCCACCT GCAGCTTCAC CTATCCGATC CGGGCCGGGA ACGACCCGGG   661 CGTGGCGCCG GGCGGCACGG GCGGANGCCT CCTCTATGGC ANGGAGTCCG CTCCCCCTCC   721 GACGGCTCCC TTCAACCTGG CGGANATCAA CGACGTGAGC CCCTCGGGCG GCTTCGTGGC   781 CGANCTCCTG CGGCCAGAAT TGGACCCGGT GTACATTCCG CCGCAGCANC CGCANNCGCC   841 AGGTGGCGGG CTGATGGGCA AGTTCGTGCT GAANGCGTCG CTGANCGCCC CNNNNANCGA   901 GTACGGNAGC CCGNNNNNCA TCANCGNCAG CAAANGNANC CNNNANNNCA NNNACCCNGN 961 NGGNGGNNNN NNCNNNNNNN NGNNNGNNNN NNNNNN //
Primer: hKLF4-mut check_R2 (Pr1009) Region: insert 3'(hKLF4(K443A) 3'),3' LTR Sequence file: RDB19008_B1Ecb.seq
>D07716A5_B1Ec_1_hKLF4-mut check_R2_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNN NGGGGCCNNA TTACCCNTCC TTCCTGCCCG    61 ATCAGATGCA GCCGCAAGTC CCGCCGCTCC ATTACCAAGA GCTCATGCCA CCCGGTTCCT   121 GCATGCCAGA GGAGCCCAAG CCAAAGAGGG GAAGACGATC GTGGCCCCGG AAAAGGACCG   181 CCACCCACAC TTGTGATTAC GCGGGCTGCG GCAAAACCTA CACAGCGAGT TCCCATCTCA   241 AGGCACACCT GCGAACCCAC ACAGGTGAGA AACCTTACCA CTGTGACTGG GACGGCTGTG   301 GATGGAAATT CGCCCGCTCA GATGAACTGA CCAGGCACTA CCGTAAACAC ACGGGGCACC   361 GCCCGTTCCA GTGCCAAAAA TGCGACCGAG CATTTTCCAG GTCGGACCAC CTCGCCTTAC   421 ACATGAAGAG GCATTTTTAA GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT   481 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA   541 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC   601 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA   661 AGCAGTTCCT GCCCCGGCTC ANGGCCAAGA ACAGATGGAA CAGCTGANTA TGGGCCAAAC   721 AGGATATCTG TGGTAAGCAG TTCCTGNCCC GGCTCANGGC CNAGAACAGA TGGTCCCCAG   781 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA NGTTTCCNGG GNGCCCCNAG   841 GANCTGAAAT GANCCTGNGC CNTATTTGAA CNNACCNATC AGTNCGCTNN NCGCTTNNGN 901 NCNCGCGCTT CNGCTCCCNN NNNNCNNNAN ANNANNNNNN NNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert mid(hKLF4(K443A) mid) Sequence file: RDB19008_B1Ecc.seq
>D07716A5_B1Ec_1_pMXs-3LTRup_R2_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNCGTCNN NCNCTNNNNN GGCGGCCGCT TANNNNNGCC    61 TCTTCNTGTG TAAGGCGAGG TGGTCCGACC TGGAAAATGC TCGGTCGCAT TTTTGGCACT   121 GGAACGGGCG GTGCCCCGTG TGTTTACGGT AGTGCCTGGT CAGTTCATCT GAGCGGGCGA   181 ATTTCCATCC ACAGCCGTCC CAGTCACAGT GGTAAGGTTT CTCACCTGTG TGGGTTCGCA   241 GGTGTGCCTT GAGATGGGAA CTCGCTGTGT AGGTTTTGCC GCAGCCCGCG TAATCACAAG   301 TGTGGGTGGC GGTCCTTTTC CGGGGCCACG ATCGTCTTCC CCTCTTTGGC TTGGGCTCCT   361 CTGGCATGCA GGAACCGGGT GGCATGAGCT CTTGGTAATG GAGCGGCGGG ACTTGCGGCT   421 GCATCTGATC GGGCAGGAAG GATGGGTAAT TGGGCCCCGG GTGGGGATGG AAGCCGGGAG   481 GAAGCGGCAG GGCAGGGTGA CAGTCCCTGC TGCTCAGCAC TTCCTCNNNA CCCAGGGTCG   541 GGGTAGTCCT GCTGGGGAGC TGCCGCCCCA GGGGGAAGTC GTGTGCAGCC GGCCGGTGGC   601 CATTGCTGAG AGGGGGTCCA GCGCCCAAGT GGGTGCACGA AGAGACCGCC TCCTGCTTGA   661 TCTTGGGGCA CGTGCGCGGC GGCCCGCCGT TGTAGGGCGC CACCACCACC GGGTGGCTGC   721 CGTCAGGGCT GCCTTTGCTG ACNCTGATGA CCGACGGGCT GCCGTACTCG CTGCCNGGGG   781 CGCTCAGCGA CGCCTTCAGC ACGAACTTGC CCATCANNCN GCCACCTGGC GGCTGNGGCT   841 GCTGCGGCGG ANNGTACACC NGGNNCNNTN NNGGCNNCNN NANCTCNNNN NNNAANNCNC   901 NCNNANGGNN TCANGTNNNT NANGTCNNCN NGNNNNAANG NNNCCGNCGG NANGGGGANC 961 NGNANNNCNN NNNNTNNNNN NNNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19008_B1Ecd.seq
>D07716A5_B1Ec_1_gag-N_R_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTNNTT TTNNGANNAA TACNGAAACN CAGTCAGACA    61 GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC GGTCCCAAAC   121 CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC CTACCAGAAC   181 CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG GATTTTTGGA   241 CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA GGGTTGCGGC   301 CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC TAGTTAGCTA   361 ACTAGTACCG ACGCAGGCGC ATAAAATCAG TCATAGACAC TAGACAATCG GACAGACACA   421 GATAAGTTGC TGGCCAGCTT ACCTCCCGGT GGTGGGTCGG TGGTCCCTGG GCAGGGGTCT   481 CCCGATCCCG GACGAGCCCC CAAATGAAAG ACCCCCGCTG ACGGGTAGTC AATCACTCAG   541 AGGAGACCCT CCCAAGGAAC AGCGAGACCA CAAGTCGGAT GCAACTGCAA GAGGGTTTAT   601 TGGATACACG GGTACCCGGG CGACTCAGTC AATCGGAGGA CTGGCGCGCC GAGTGAGGGG   661 TTGTGGGCTC TTTTATTGAG CTCGGGGAGC AGAAGCGCGC GAACAGAAGC GAGAAGCGAA   721 CTGATTGGTT AGTTCAAATA AGGCACAGGG TCATTTCAGG TCCTTGGGGC ACCCTGGANA   781 CATCTGATGG TTCTCTAGAA ACTGCTGANG GCTGGACCGC ATCTGGGGAC CATCTGTTCT   841 TGGCCCTGAN CCGGGGCANG AACTGCTTAC CNCAGATATC CTGTTNGGNC CATATTCAGC   901 NGTNCCATCT NNNCNNGGCC NNNANCCGGG NNANGNNCNG NNTANNNCAG ATATCCNGNT 961 NNNNCNNTNT TCNGNNNNNN NNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19008_B1Ece.seq
>D07716A5_B1Ec_1_lacIpro-B_F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC ANCNNNNCNG TGANCGANGA NCGGAAGANC GCCCAATACG    61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA   121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC   181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG   241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG   301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC   361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA   421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC   481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA   541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC   601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG   661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCCATGC CTTGCAAAAT GGCGTTACTT   721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC CCCCTTTTTC TGGAGACTAA   781 ATAAAATCTT TTANTTTATC GTCGACCACT GTGCTGGCGG CCGCTTNAAA ATGCCTCTTC   841 ATGTGTAANG NGAGGTGGTC CGACCTGNAA AATGNTCNGT CGCATTTTTN GGNACTNGAA   901 CGGGCGGNGC CCCGNGTGTT TANNNNANTG NCTGNNCANN NNNTNNNANC GGGNNNNNTT 961 NCNTNCNCAG NCNTCCCAGT NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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