Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(H446A)

Retroviral expression vector of human KLF4, mutant H446A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

Tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19009_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(H446A) 5') Sequence file: RDB19009_B1Eca.seq
>D07716A6_B1Ec_1_MMLV-pol_F_D02_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGGC ATCGCANCTT GGNNNCACGC CGCCCACGTG AAGGCTGCCG    61 ACCCCGGGGG TGGANCATCC TCTAGACTGC CGGATCTAGC TAGTTAATTA AGGATCCCAG   121 TGTGGTGGTA CGGGAATTCA TGGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA   181 CATCGACTAC AAGGATGACG ATGACAAGGC TGTCAGCGAC GCGCTGCTCC CATCTTTCTC   241 CACGTTCGCG TCTGGCCCGG CGGGAAGGGA GAAGACACTG CGTCAAGCAG GTGCCCCGAA   301 TAACCGCTGG CGGGAGGAGC TCTCCCACAT GAAGCGACTT CCCCCAGTGC TTCCCGGCCG   361 CCCCTATGAC CTGGCGGCGG CGACCGTGGC CACAGACCTG GAGAGCGGCG GAGCCGGTGC   421 GGCTTGCGGC GGTAGCAACC TGGCGCCCCT ACCTCGGAGA GAGACCGAGG AGTTCAACGA   481 TCTCCTGGAC CTGGACTTTA TTCTCTCCAA TTCGCTGACC CATCCTCCGG AGTCAGTGGC   541 CGCCACCGTG TCCTCGTCAG CGTCAGCCTC CTCTTCGTCG TCGCCGTCGA GCAGCGGCCC   601 TGCCAGCGCG CCCTCCACCT GCAGCTTCAC CTATCCGATC CGGGCCGGGA ACGACCCGGG   661 CGTGGCGCCG GGCGGCACGG GCGGANGCCT CCTCTATGGC AGGGAGTCCG CTCCCCCTCC   721 GACGGCTCCC TTCAACCTGG CGGACATCAA CGACGTGAGC CCCTCGGGCG GCTTCGTGGC   781 CGANCTCCTG CGGCCAGAAT TGGACCCGGT GTACATTCCG CCGCANCAGC CGCAGCCGCC   841 AGGTGGCGGG CTGATGGGNA AGTTCGNGCT GANNGCGTCG CTGANCGCCC NNNGNANCGA   901 GTACNGNAGN CNNNCNGNCA TCANCGNCAN NNAANGNAGC CNNNANNNNA GCCACCNGGN 961 GNNNGNNNNN NNNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert 3'(hKLF4(H446A) 3') Sequence file: RDB19009_B1Ecb.seq
>D07716A6_B1Ec_1_pMXs-3LTRup_R2_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTNN NCGTCGNNNN CTGTGCTGGC GGCCGCTTAA AAATGCCTCT    61 TCATGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA   121 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGAATT   181 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT   241 GTGCCTTGAG AGCGGAACTC TTTGTGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT   301 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG   361 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA   421 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA   481 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG   541 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT   601 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT   661 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT ANGGCGCCAC CACCACCGGG TGGCTGCCGT   721 CAGGGCTGCC TTTGCTGACG CTGATGANCG ACGGGCTGCC GTACTCGCTG CCAGGGGCGC   781 TCAGCGACGC CTTCAGCACG AACTTGCCCA TCAGCCCGCC ACCTGGCGGC TGCGGCTGCT   841 GCGGCGGANN GNACACCGNN NCCAATTCTG GNCGCNNNAN CTCNGNCACN AANCCGCCCN   901 ANGGGNNCAC NNNNNNNATG NCCGNCAGGN NNNANNGNNN CCNNNNNANG GGNNNCNGNN 961 NTNCNNNNNN NNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19009_B1Ecc.seq
>D07716A6_B1Ec_1_gag-N_R_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN NTAATTTTNN GANNAATACA GAAACACAGT CAGACAGAGA    61 CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC GGCTTCGGTC CCAAACCGAA   121 AGCAAAAATT CAGACGGAGG CGGGAACTGT TTTAGGTTCT CGTCTCCTAC CAGAACCACA   181 TATCCCTCCT ATTAGGGGGG TGCACCAAAG AGTCCAAAAC GATCGGGATT TTTGGACTCA   241 GGTCGGGCCA CAAAAACGGC CCCCGAAGTC CCTGGGACGT CTCCCAGGGT TGCGGCCGGG   301 TGTTCCGAAC TCGTCAGTTC CACCACGGGT CCGCCAGATA CAGAGCTAGT TAGCTAACTA   361 GTACCGACGC AGGCGCATAA AATCAGTCAT AGACACTAGA CAATCGGACA GACACAGATA   421 AGTTGCTGGC CAGCTTACCT CCCGGTGGTG GGTCGGTGGT CCCTGGGCAG GGGTCTCCCG   481 ATCCCGGACG AGCCCCCAAA TGAAAGACCC CCGCTGACGG GTAGTCAATC ACTCAGAGGA   541 GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG GTTTATTGGA   601 TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCGCCGAGT GAGGGGTTGT   661 GGGCTCTTTT ATTGAGCTCG GGGAGCAGAA GCGCGCGAAC AGAAGCGAGA AGCGAACTGA   721 TTGGTTAGTT CAAATAAGGC ACAGGGTCAT TTCAGGTCCT TGGNGCACCC TGGAAACATC   781 TGATGGNTCT CTAGAAACTG CTGANGGCTG GANCGCATCT GGGGACCATC TGTTCTTGGC   841 CCTGNNCCNG GGCANGAACT GCTTANCACA NATATNCTGT TNGGCCCATA TTCAGCTGNN   901 NCNTCTGNNC TTGGNCCNGA NNCGGGGNNN GNANNNGNTN ANNNNAGATN TCCNGNTTNG 961 NCNNNNNNNN NN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR,insert 3'(hKLF4(H446A) 3') Sequence file: RDB19009_B1Ecd.seq
>D07716A6_B1Ec_1_lacIpro-B_F_E02_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGC NNNNNNNCNG TGNGCGAGGA AGCGGAAGAN CGCCCAATAC    61 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC   481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT   541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA   601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT   661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGGCGTTACT   721 TAAGCTAGCT TGCCAAACCT ACAGGTGGGG TCTTTCATTC CCCCCTTTTT CTGGAGACTA   781 AATAAAATCT TTTATTTTAT CGTCGACCAC TGTGCTGGCG GCCGCTTAAA AATGCCTCTT   841 CATGTGTAAN GCGAGGTGGT CCGANCTGNA AAATGCTCGG TCGCATTTTT TGGCNNNNNA   901 ACGGGCGGNG CCCCGNGNGT TTACNNNANT GCCNNNNNAN NTCNTNTGAN CGGGNGANNT 961 TCCATCCACA NNCNNTNCNN NNCNNNNNNN NNANNNNTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

GNP_filter3_RDBDEP_html_230702.pl