Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(F471A)

Retroviral expression vector of human KLF4, mutant F471A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19011_B1Ecp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5'(3xFLAG,hKLF4(F471A) 5') Sequence file: RDB19011_B1Eca.seq
>D07716A8_B1Ec_2_MMLV-pol_F_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNTCGCANC TTGGNNACNC GCCGCCCACG TGAAGGCTGC    61 CGACCCCGGG GGTGGACCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC   121 AGTGTGGTGG TACGGGAATT CATGGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT   181 GACATCGACT ACAAGGATGA CGATGACAAG GCTGTCAGCG ACGCGCTGCT CCCATCTTTC   241 TCCACGTTCG CGTCTGGCCC GGCGGGAAGG GAGAAGACAC TGCGTCAAGC AGGTGCCCCG   301 AATAACCGCT GGCGGGAGGA GCTCTCCCAC ATGAAGCGAC TTCCCCCAGT GCTTCCCGGC   361 CGCCCCTATG ACCTGGCGGC GGCGACCGTG GCCACAGACC TGGAGAGCGG CGGAGCCGGT   421 GCGGCTTGCG GCGGTAGCAA CCTGGCGCCC CTACCTCGGA GAGAGACCGA GGAGTTCAAC   481 GATCTCCTGG ACCTGGACTT TATTCTCTCC AATTCGCTGA CCCATCCTCC GGAGTCAGTG   541 GCCGCCACCG TGTCCTCGTC AGCGTCAGCC TCCTCTTCGT CGTCGCCGTC GAGCAGCGGC   601 CCTGCCAGCG CGCCCTCCAC CTGCAGCTTC ACCTATCCGA TCCGGGCCGG GAACGACCCG   661 GGCGTGGCGC CGGGCGGCAC GGGCGGAGGC CTCCTCTATG GCAGGGAGTC CGCTCCCCCT   721 CCGACGGCTC CCTTCAACCT GGCGGANATC AACGACGTGA GCCCCTCGGG CGGCTTCGTG   781 GCCGANCTCC TGCGGCCAGA ATTGGACCCG GNGTACATTC CGCCGCANCA NCCGCAGCCG   841 CCAGGTGGCG GGCTGATGNG CAAGTTCGTG CTGAANGNGT CNCTGANCGC CCCTNNNANC   901 GANNACGGNN NCCCGNCNGN CATCANCGTC AGCAANNGNA NNCNNNANNN NNNCCNCCCN 961 GNNNNNNNNN N //
Primer: hKLF4-mut check_R2 (Pr1009) Region: insert 3'(hKLF4(F471A) 3'),3' LTR Sequence file: RDB19011_B1Ecb.seq
>D07716A8_B1Ec_2_hKLF4-mut check_R2_A04_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN NNNNNNNNNG GGGCCCNNTT ACCCATCCTT CCTGCCCGAT    61 CAGATGCAGC CGCAAGTCCC GCCGCTCCAT TACCAAGAGC TCATGCCACC CGGTTCCTGC   121 ATGCCAGAGG AGCCCAAGCC AAAGAGGGGA AGACGATCGT GGCCCCGGAA AAGGACCGCC   181 ACCCACACTT GTGATTACGC GGGCTGCGGC AAAACCTACA CAAAGAGTTC CCATCTCAAG   241 GCACACCTGC GAACCCACAC AGGTGAGAAA CCTTACCACT GTGACTGGGA CGGCTGTGGA   301 TGGAAAGCCG CCCGCTCAGA TGAACTGACC AGGCACTACC GTAAACACAC GGGGCACCGC   361 CCGTTCCAGT GCCAAAAATG CGACCGAGCA TTTTCCAGGT CGGACCACCT CGCCTTACAC   421 ATGAAGAGGC ATTTTTAAGC GGCCGCCAGC ACAGTGGTCG ACGATAAAAT AAAAGATTTT   481 ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC   541 TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG AGAAGTTCAG   601 ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG   661 CAGTTCCTGC CCCGGCTCAN GGCCAAGAAC AGATGGAACA GCTGAATATG GGCCAAACAG   721 GATATCTGTG GTAAGCAGTT CCTGNCCCGG CTCNNGGCCA AGAACAGATG GTCCCCAGAT   781 GCGGTCCAGC CCTCAGCAGT TTCTAGAGAA CCATCAGANG TTTCCAGGGT GCCCCANNGA   841 CCTGAAATGA CCCTGTGNNT TATTTGAACT ANCCAATCAG TTCGCTTCTC GCTTCTGNTC   901 GCGCGCTTCT GNTCCCCGAN CNNNNTAAAA GANCCCACNN NCNCTCANNC NGGGNNNNNN 961 NNNN //
Primer: pMXs-3LTRup_R2 (Pr0365) Region: insert mid(hKLF4(F471A) mid) Sequence file: RDB19011_B1Ecc.seq
>D07716A8_B1Ec_2_pMXs-3LTRup_R2_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTNN NCGTCGNNNN CTGTGCTGGC GGCCGCTTAA AAATGCCTCT    61 TCNTGTGTAA GGCGAGGTGG TCCGACCTGG AAAATGCTCG GTCGCATTTT TGGCACTGGA   121 ACGGGCGGTG CCCCGTGTGT TTACGGTAGT GCCTGGTCAG TTCATCTGAG CGGGCGGCTT   181 TCCATCCACA GCCGTCCCAG TCACAGTGGT AAGGTTTCTC ACCTGTGTGG GTTCGCAGGT   241 GTGCCTTGAG ATGGGAACTC TTTGTGTAGG TTTTGCCGCA GCCCGCGTAA TCACAAGTGT   301 GGGTGGCGGT CCTTTTCCGG GGCCACGATC GTCTTCCCCT CTTTGGCTTG GGCTCCTCTG   361 GCATGCAGGA ACCGGGTGGC ATGAGCTCTT GGTAATGGAG CGGCGGGACT TGCGGCTGCA   421 TCTGATCGGG CAGGAAGGAT GGGTAATTGG GCCCCGGGTG GGGATGGAAG CCGGGAGGAA   481 GCGGCAGGGC AGGGTGACAG TCCCTGCTGC TCAGCACTTC CTCAAGACCC AGGGTCGGGG   541 TAGTCCTGCT GGGGAGCTGC CGCCCCAGGG GGAAGTCGTG TGCAGCCGGC CGGTGGCCAT   601 TGCTGAGAGG GGGTCCAGCG CCCAAGTGGG TGCACGAAGA GACCGCCTCC TGCTTGATCT   661 TGGGGCACGT GCGCGGCGGC CCGCCGTTGT AGGGCGCCAC CACCACCGGG TGGCTGCCGT   721 CAGGGCTGCC TTTGCTGACG CTGATGACCG ACGGGCTGCC GTACTCGCTG CCAGGGGCGC   781 TCAGCGACGC CTTCNGCACG AACTTGCCCA TCAGCCCGCC ACCTGGCGGC TGCNNNTGCT   841 GCGGCNNAAN GTACACCNGG NCCNATTCTG GNCGCANGAN CTCGGCCNCN AAGCCGCCCG 901 ANGGGNTCAC GTNNTTGATG NNCGCNNNGT TGNANNNNNN NNNN //
Primer: gag-N_R (Pr0745) Region: MMLV psi,5' LTR Sequence file: RDB19011_B1Ecd.seq
>D07716A8_B1Ec_2_gag-N_R_H03_24_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCNG NNNNNAATTT TNNGANNNNN ANNGAAACNC AGTCAGACAG    61 AGACAACACA GAACGATGCT GCAGCAGACA AGACGCGCGG CGCGGCTTCG GTCCCAAACC   121 GAAAGCAAAA ATTCAGACGG AGGCGGGAAC TGTTTTAGGT TCTCGTCTCC TACCAGAACC   181 ACATATCCCT CCTATTAGGG GGGTGCACCA AAGAGTCCAA AACGATCGGG ATTTTTGGAC   241 TCAGGTCGGG CCACAAAAAC GGCCCCCGAA GTCCCTGGGA CGTCTCCCAG GGTTGCGGCC   301 GGGTGTTCCG AACTCGTCAG TTCCACCACG GGTCCGCCAG ATACAGAGCT AGTTAGCTAA   361 CTAGTACCGA CGCAGGCGCA TAAAATCAGT CATAGACACT AGACAATCGG ACAGACACAG   421 ATAAGTTGCT GGCCAGCTTA CCTCCCGGTG GTGGGTCGGT GGTCCCTGGG CAGGGGTCTC   481 CCGATCCCGG ACGAGCCCCC AAATGAAAGA CCCCCGCTGA CGGGTAGTCA ATCACTCAGA   541 GGAGACCCTC CCAAGGAACA GCGAGACCAC AAGTCGGATG CAACTGCAAG AGGGTTTATT   601 GGATACACGG GTACCCGGGC GACTCAGTCA ATCGGAGGAC TGGCGCGCCG AGTGAGGGGT   661 TGTGGGCTCT TTTATTGAGC TCGGGGAGCA GAAGCGCGCG AACAGAAGCG AGAAGCGAAC   721 TGATTGGTTA GTTCAAATAA GGCACAGGGT CATTTCAGGT CCTTGGGGCA CCCTGGAAAC   781 ATCTGATGGN TCTCTAGAAA CTGCTGANGG CTGGACCGCA TCTGGGGANC ATCTGTTCTT   841 GGCCCTGANN NGGGGNANGA ACTGCTNANC ACAGATATCC TGTTTNGCCN ATATTNNGCT   901 GNTCCATCNG NTCTTGGCCC NNANNNNGGG GCNNNNNCTG NNNANCNNNG ATATNCNNNT 961 TNGNNNNNNN NNCNNNNGNT NNNNNNNNNN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB19011_B1Ece.seq
>D07716A8_B1Ec_2_lacIpro-B_F_G03_21_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NCAGTGANCG AGGNAGCGGN ANNAGCGCCC    61 NATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGANNNNN TAATGCAACT ATGGCCATTT   121 NNNGTAAATA CTTAAGAAAA AAAACCAAAT TAATTTTGAT ACATGCTGCA TGTGAAAGAC   181 CCCCGCTGAC GGGTAGTCAA TCACTCAGAG GAGACCCTCC CAAGGAACAG CGAGACCACA   241 AGTCGGATGC AACTGCAAGA GGGTTTATTG GATACACGGG TACCCGGGCG ACTCAGTCAA   301 TCGGAGGACT GGCGCCCCGA GTGAGGGGTT GTGGGCTCTT TTATTGAGCT CGGGGAGCAG   361 AAGCGCGCGA ACAGAAGCGA GAAGCGAACT GATTGGTTAG TTCAAATAAG GCACAGGGTC   421 ATTTCAGGTC CTTGGGGCAC CCTGGAAACA TCTGATGGTT CTCTAGAAAC TGCTGAGGGC   481 TGGACCGCAT CTGGGGACCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA   541 CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCTTGGCCCT GAGCCGGGGC   601 AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCCTG   661 ACCTTGATCT GAACTTCTCT ATTCTCAGTT ATGTATTTTT CCATGCCTTG CAAAATGGNG   721 TTACTTAAGC TAGCTTGCCA AACCTACAGG TGGGGTCTTT CATTCCCCCC TTTTTCTGGA   781 GACTAAATAA AATCTTTTAT TTTATCGTCN ACCACTGTGC TGGCGGCCGC TNNANNAATG   841 CCTCTTCATG TGTAANGCGA GGTGGNNCGA NCNTGNAAAA TGCTCNGGTC GCATTTTTGN   901 NANTGGNAAN NGGGCGGTNN CCNNNTGTGT TTANNGGTAN TNNCNGGNNC NNNTNNNTNN   961 NGANNNGGCN GNNTTTNCNA TCCNNAGCNN NTCCCANTCN NANNGGNAAN GGNTTNNNNN 1021 NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.29

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